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- Pavlovic, M.; Luze, A.; Konrad, R.; Berger, A.; Sing, A.; Busch, U.; Huber, I.
- Journal of applied microbiology 2011 v.110 no.5 pp. 1245-1251
- enteroinvasive Escherichia coli, etc ; Shigella; beta-glucuronidase; genes; lactose; quantitative polymerase chain reaction; serotypes; Show all 7 Subjects
- ... Aims: The aim of this study was to develop a real-time PCR test for differentiation between Shigella spp. and E. coli, in particular enteroinvasive Escherichia coli (EIEC). Methods and Results: A duplex real-time PCR specific for the genes encoding for β-glucuronidase (uidA) and lactose permease (lacY) was developed. Ninety-six isolates including 11 EIEC isolates of different serotypes and at leas ...