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- Kimura, B.; Takahashi, H.; Hokimoto, S.; Tanaka, Y.; Fujii, T.
- Journal of applied microbiology 2009 v.107 no.2 pp. 485-497
- histidine; histidine decarboxylase, etc ; Escherichia coli; Gram-negative bacteria; Northern blotting; Photobacterium damselae subsp. damselae; antiporters; histamine; multigene family; open reading frames; operon; pH; plasmids; polypeptides; quantitative analysis; reverse transcriptase polymerase chain reaction; Show all 16 Subjects
- ... To elucidate the detailed mechanism of histamine production by Photobacterium damselae subsp. damselae. Histidine decarboxylase and related genes of P. damselae subsp. damselae were cloned, and three open reading frames named as hdcT, hdcA and hisRS were identified. The hdcA gene encodes a polypeptide of 377 amino acids and is considered to be the pyridoxal-P dependent histidine decarboxylase. The ...
- Leisner, J.J.; Larsen, M.H.; Ingmer, H.; Petersen, B.O.; Duus, J.Ø.; Palcic, M.M.
- Journal of applied microbiology 2009 v.107 no.6 pp. 2080-2087
- histidine, etc ; Enterococcus faecalis; Listeria monocytogenes; amino acid sequences; catalytic activity; chitinase; enzyme activity; genes; hosts; molecular weight; pH; peptidoglycans; phylogeny; polymerase chain reaction; temperature profiles; Show all 15 Subjects
- ... To compare enzymatic activities of two related chitinases, ChiA and EF0361, encoded by Listeria monocytogenes and Enterococcus faecalis, respectively. The chiA and EF0361 genes were amplified by PCR, cloned and expressed with histidine tags, allowing easy purification of the gene products. ChiA had a molecular weight as predicted from the amino acid sequence, whereas EF0361 was 1840 Da lower than ...