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- Lindqvist, R.
- Journal of applied microbiology 1999 v.86 no.6 pp. 971-978
- enteroinvasive Escherichia coli, etc ; Shigella flexneri; blue cheese; centrifugation; lettuce; loci; milk; plasmids; polymerase chain reaction; shrimp; Show all 10 Subjects
- ... A nested PCR method was developed and its performance evaluated by detection of Shigella flexneri in food. The nested PCR amplifies sequences within an invasion-associated locus (ial) on the invasion plasmid specific for Shigella and enteroinvasive Escherichia coli (EIEC). The nested PCR detected Sh. flexneri in lettuce inoculated with 2, 20 and 200 cfu g-1 after 1, 7 and 18 d of storage, respecti ...