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- Matsumura, Kenji; Yamada, Miwa; Yamashita, Takeshi; Muto, Hitomi; Nishiyama, Ken-ichi; Shimoi, Hitoshi; Isobe, Kimiyasu
- Journal of bioscience and bioengineering 2019 v.128 no.1 pp. 13-21
- Escherichia coli; Ochrobactrum; alcohol oxidase; consensus sequence; cytoplasm; genes; glycolic acid; glyoxylic acid; nucleotides; open reading frames; pH stability; substrate specificity
- ... We cloned a set of genes encoding alcohol oxidase from Ochrobactrum sp. AIU 033 (OcAOD), which exhibits the appropriate substrate specificity for glyoxylic acid production from glycolic acid. The set of genes for OcAOD contained two open reading frames consisting of 555-bp (aodB) and 1572-bp (aodA) nucleotides, which encode the precursor for the β-subunit and α-subunit of OcAOD, respectively. We e ...