Jump to Main Content
- Scharf, Klaus-Dieter; Höhfeld, Ingo; Nover, Lutz
- Journal of biosciences 1998 v.23 no.4 pp. 313-329
- DNA-binding domains; amino acids; cell culture; clones; complementary DNA; cytoplasmic granules; gene expression; genes; heat; heat shock proteins; heat shock response; heat stress; helix-turn-helix motifs; mutants; oligomerization; peptides; physiological transport; protoplasts; tobacco; tomatoes; transcription factors; yeasts
- ... Expression of heat shock protein (HSP)-coding genes is controlled by heat stress transcription factors (Hsfs). They are structurally and functionally conserved throughout the eukaryotic kingdom. In addition to the DNA-binding domain with the helix-turn-helix motif essential for DNA recognition, three functional parts in the C-terminal activator domain were characterized: (i) the HR-A/B region is r ...
- Srinivas, Usha K; Swamynathan, S K; Revathi, C J
- Journal of biosciences 1998 v.23 no.4 pp. 387-398
- Northern blotting; adults; albumins; antibodies; binding sites; chloramphenicol acetyltransferase; embryogenesis; gel electrophoresis; heat; heat shock proteins; heat stress; hepatocytes; immunoblotting; liver; messenger RNA; nucleotide sequences; oligonucleotides; plasmids; pregnancy; rats; reporter genes; specific heat; temperature; transcription (genetics); transcription factors; transfection
- ... Albumin is an adult liver specific protein whose induction in rats starts at day 19 or 20 of normal gestation. Our studies on the effect of heat stress on embryonic development showed premature induction of a 67 kDa protein at day 12 or 13 in embryonic liver cells, in addition to the induction of usual heat shock proteins. Immunoblotting with anti-albumin antibody confirmed the prematurely induced ...