Jump to Main Content
- Gundinger, Thomas; Spadiut, Oliver
- Journal of biotechnology 2017
- Escherichia coli; bacteria; catalytic activity; directed evolution; engineering; inclusion bodies; peroxidase; screening; thermal stability
- ... Horseradish peroxidase (HRP) is used in various biotechnological and medical applications. Since its isolation from plant provides several disadvantages, the bacterium Escherichia coli was tested as recombinant expression host in former studies. However, neither production from refolded inclusion bodies nor active enzyme expression in the periplasm exceeded final titres of 10mg per litre cultivati ...
- PubMed Central: