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- Deng, Lan; Mori, Yutaka; Sermsathanaswadi, Junjarus; Apiwatanapiwat, Waraporn; Kosugi, Akihiko
- Journal of biotechnology 2015 v.212 pp. 144-152
- cellulosome, etc ; Clostridium thermocellum; Halocynthia; carboxymethylcellulose; cellulose; hydrolysis; mutants; polyacrylamide gel electrophoresis; scaffolding proteins; Show all 9 Subjects
- ... Mutant Clostridium thermocellum YM72 that produces small-size scaffolding protein CipA (ssCipA) was isolated from wild-type YM4. Sequencing of ssCipA revealed that two domains, cohesin 6 and cohesin 7, were not present. Cellulosome prepared from YM72 exhibited a significant reduction of hydrolysis ability on crystalline celluloses such as Sigmacell type-20 and cellulose from Halocynthia. To invest ...