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You searched for: Journal Journal of biotechnology Remove constraint Journal: Journal of biotechnology Publication Year 2010 Remove constraint Publication Year: 2010 Source 2010 v.145 no.1 Remove constraint Source: 2010 v.145 no.1
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1. An improved and cost-effective cDNA-AFLP method to investigate transcription-derived products when high throughput sequencing is not available

Author:
Korpelainen, Helena; Kostamo, Kirsi
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 43-46
ISSN:
0168-1656
Subject:
biotechnology; cost effectiveness; genes; polymerase chain reaction
Abstract:
... Although the cost of high throughput sequencing is decreasing, the cost is still often too high for individual projects targeted at, e.g., genome-wide transcription profiling in non-model organisms. Then, a low-cost alternative is cDNA-AFLP, which we have now considerably modified in order to develop a faster and simpler method to identify and analyze genes involved in specific, possibly adaptive ...
DOI:
10.1016/j.jbiotec.2009.10.006

2. A novel anti-EGFR monoclonal antibody inhibiting tumor cell growth by recognizing different epitopes from cetuximab

Author:
Hong, Kwang-Won; Kim, Chang-Goo; Lee, Seung-Hyun; Chang, Ki-Hwan; Shin, Yong Won; Ryoo, Kyung-Hwan; Kim, Se-Ho; Kim, Yong-Sung
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 84-91
ISSN:
0168-1656
Subject:
anticarcinogenic activity; biotechnology; breast neoplasms; cell growth; enzyme-linked immunosorbent assay; epidermal growth factor receptors; epitope mapping; epitopes; humans; lung neoplasms; mice; monoclonal antibodies; neoplasm cells; phosphorylation; placenta; surface plasmon resonance; therapeutics; tissues; tyrosine; uterine cervical neoplasms
Abstract:
... The epidermal growth factor receptor (EGFR) overexpressed in many epithelial tumors is an attractive target for tumor therapy since numerous blocking agents of EGFR signaling have proven their anti-tumor activity. Here we report a novel monoclonal antibody (mAb), A13, which was generated from mice immunized with human cervical carcinoma A431 cells. In addition to binding to soluble EGFR with affin ...
DOI:
10.1016/j.jbiotec.2009.09.023

3. A novel economic method for high throughput production of recombinant baculovirus by infecting insect cells with Bacmid-containing diminopimelate-auxotrophic Escherichia coli

Author:
Yao, Lun guang; Sun, Jing chen; Xu, Hua; Kan, Yun chao; Zhang, Xiang man; Yan, Hui Chao
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 23-29
ISSN:
0168-1656
Subject:
Baculoviridae; DNA; Escherichia coli; Spodoptera frugiperda; Yersinia; bacteria; biotechnology; cell walls; cost effectiveness; insects; transfection
Abstract:
... Developing cost-effective methods for high throughput production of recombinant baculoviruses in insect cells is very challenging, because the baculovirus DNA preparation and the following transfection procedure are labour-intensive and time consuming. We developed a new method of introducing recombinant Bacmid DNA from bacteria into insect cells simply using invasive diaminopimelate (DAP) auxotro ...
DOI:
10.1016/j.jbiotec.2009.10.003

4. ATP-dependent stabilization and protection of fibroblast growth factor 2

Author:
Rose, Karsten; Gast, Ronald E.; Seeger, Anna; Krieglstein, Josef; Klumpp, Susanne
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 54-59
ISSN:
0168-1656
Subject:
adenosine diphosphate; adenosine monophosphate; adenosine triphosphate; alkaline phosphatase; angiogenesis; biotechnology; cell culture; cell proliferation; clinical trials; digestion; elastase; fibroblast growth factor 2; heat; human umbilical vein endothelial cells; in vitro digestion; mutants; neuroprotective effect; nucleosides; plasmin; sodium tripolyphosphate; tissue repair; trypsin
Abstract:
... Fibroblast growth factor 2 (FGF2) plays a pivotal role in cell proliferation, angiogenesis and neuroprotection. Several clinical trials using this growth factor in bone regeneration, wound healing and cardioprotection are initiated but the inadequate stability of FGF2 after application is one major problem. Binding of ATP to FGF2 and other growth factors has been demonstrated recently. Here we rep ...
DOI:
10.1016/j.jbiotec.2009.10.005

5. Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction

Author:
Tanaka, Tsutomu; Masunari, Shinsuke; Ishii, Jun; Wakamura, Kanako; Segawa, Maiko; Fukuda, Hideki; Kondo, Akihiko
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 79-83
ISSN:
0168-1656
Subject:
Escherichia coli; biotechnology; biotin; streptavidin; yeasts
Abstract:
... Here we expand the yeast cell surface display system to display non-natural, functional molecules. The short biotin acceptor peptide (BAP) sequence of biotin ligase from E. coli (BirA) was genetically introduced to the N-terminus of the anchor protein, Flo428. Through co-expression of BAP-fused Flo428 with BirA, biotinylated BAP could be displayed on the yeast cell surface. Subsequent addition of ...
DOI:
10.1016/j.jbiotec.2009.10.011

6. Preparation and characterization of folate-poly(ethylene glycol)-grafted-trimethylchitosan for intracellular transport of protein through folate receptor-mediated endocytosis

Author:
Zheng, Yu; Song, Xiangrong; Darby, Michael; Liang, Yufeng; He, Ling; Cai, Zheng; Chen, Qiuhong; Bi, Yueqi; Yang, Xiaojuan; Xu, Jiapeng; Li, Yuanbo; Sun, Yiyi; Lee, Robert J.; Hou, Shixiang
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 47-53
ISSN:
0168-1656
Subject:
biopharmaceuticals; biotechnology; bovine serum albumin; composite polymers; electrolytes; endocytosis; ethylene glycol; fluorescein; folic acid; neoplasm cells
Abstract:
... To develop a receptor-mediated intracellular delivery system that can transport therapeutic proteins to specific tumor cells, folate-poly(ethylene glycol)-grafted-trimethylchitosan (folate-PEG-g-TMC) was synthesized. Nano-scaled spherical polyelectrolyte complexes between the folate-PEG-g-TMC and fluorescein isothiocyanate conjugated bovine serum albumin (FITC-BSA) were prepared under suitable wei ...
DOI:
10.1016/j.jbiotec.2009.09.007

7. Preparation of ubiquitin-conjugated proteins using an insect cell-free protein synthesis system

Author:
Suzuki, Takashi; Ezure, Toru; Ando, Eiji; Nishimura, Osamu; Utsumi, Toshihiko; Tsunasawa, Susumu
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 73-78
ISSN:
0168-1656
Subject:
biotechnology; enzyme activity; insects; mass spectrometry; translation (genetics); ubiquitin; ubiquitination
Abstract:
... Ubiquitination is one of the most significant posttranslational modifications (PTMs). To evaluate the ability of an insect cell-free protein synthesis system to carry out ubiquitin (Ub) conjugation to in vitro translated proteins, poly-Ub chain formation was studied in an insect cell-free protein synthesis system. Poly-Ub was generated in the presence of Ub aldehyde (UA), a de-ubiquitinating enzym ...
DOI:
10.1016/j.jbiotec.2009.10.009

8. Quantitative considerations for suspension array assays

Author:
Connolly, Ashley R.; Palanisamy, Ramkumar; Trau, Matt
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 17-22
ISSN:
0168-1656
Subject:
DNA; RNA; biotechnology; gene expression regulation; macrophages
Abstract:
... A novel format of a multiplex microbead assay that is capable of accurately and reproducibly measuring small changes in 10⁸ copies of a nucleic acid is reported herein. The dynamic range of the assay was 32-fold greater than conventional multiplex microbead assays. The assay was modeled mathematically which enabled the accuracy of DNA quantification to be formally analysed using a logical theoreti ...
DOI:
10.1016/j.jbiotec.2009.10.004

9. Specific growth rate dependent transcriptome profiling of Escherichia coli K12 MG1655 in accelerostat cultures

Author:
Nahku, Ranno; Valgepea, Kaspar; Lahtvee, Petri-Jaan; Erm, Sten; Abner, Kristo; Adamberg, Kaarel; Vilu, Raivo
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 60-65
ISSN:
0168-1656
Subject:
Escherichia coli K12; acetates; acetic acid; bacteria; biotechnology; excretion; gene expression regulation; glucose; metabolism; microarray technology; operon; pyruvate oxidase; quantitative polymerase chain reaction; specific growth rate; transcriptomics; transporters
Abstract:
... Specific growth rate dependent gene expression changes of Escherichia coli K12 MG1655 were studied by microarray and real-time PCR analyses. The bacteria were cultivated on glucose limited minimal medium using the accelerostat method (A-stat) where starting from steady state conditions (chemostat culture) dilution rate is constantly increased. At specific growth rate (μ) 0.47h⁻¹, E. coli had focus ...
DOI:
10.1016/j.jbiotec.2009.10.007

10. An accurate normalization strategy for RT-qPCR in Hypocrea jecorina (Trichoderma reesei)

Author:
Steiger, Matthias G.; Mach, Robert L.; Mach-Aigner, Astrid R.
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 30-37
ISSN:
0168-1656
Subject:
messenger RNA; xylanases; reverse transcriptase polymerase chain reaction; gene expression; genes; Trichoderma reesei
Abstract:
... Hypocrea jecorina is an important, filamentous fungus due to its effective production of hydrolytic enzymes. Gene expression studies provide deeper insight into environment sensing and cellular response mechanisms. Reverse transcription-quantitative PCR is a gene-specific and powerful tool to measure even minor changes in mRNA composition. An accurate normalization strategy is absolutely necessary ...
DOI:
10.1016/j.jbiotec.2009.10.012
PubMed:
19861137

11. Cloning, microbial expression and structure-activity relationship of polyphenol oxidases from Camellia sinensis

Author:
Wu, Yi-Liang; Pan, Li-Ping; Yu, Si-Li; Li, Hai-Hang
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 66-72
ISSN:
0168-1656
Subject:
Camellia sinensis; tea; complementary DNA; Komagataella pastoris; catechol oxidase; gene expression; genes; structure-activity relationships; molecular cloning; enzyme activity; Escherichia coli
Abstract:
... Polyphenol oxidase (PPO) can be used for organic synthesis and degradation of wastes and dyes in industries. Lack of enzyme sources is a major barrier for its application. A PPO gene, with a full length of 1.8kb without introns, was cloned by PCR from genomic DNA of five common cultivars of Camellia sinensis. They had a 98.2-99.9% degree of identity in nucleotides and 94.7-96.1% in amino acids and ...
DOI:
10.1016/j.jbiotec.2009.10.008
PubMed:
19857531

12. Dicistronic binary vector system—A versatile tool for gene expression studies in cell cultures and plants

Author:
Ali, Zahid; Schumacher, Heinz Martin; Heine-Dobbernack, Elke; El-Banna, Antar; Hafeez, Fauzia Yusuf; Jacobsen, Hans-Jörg; Kiesecker, Heiko
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 9-16
ISSN:
0168-1656
Subject:
genetic transformation; salt tolerance; recombinant DNA; peas; transgenic plants; Agrobacterium radiobacter; luciferase; plasmid vectors; gene expression; antiporters; Nicotiana tabacum; sodium; reporter genes; Pisum sativum; promoter regions; beta-glucuronidase; tobacco
Abstract:
... Dicistronic binary vector constructs based on pGreenII vectors for Agrobacterium mediated gene transfer alleviate the translational expression monitoring of a target gene in plants. The functionality of the transformation vectors was proven by marker gene constructs containing a mannopine synthase promoter (p-MAS) fused to a beta-glucuronidase (gus) gene followed by an internal ribosome entry site ...
DOI:
10.1016/j.jbiotec.2009.10.002
PubMed:
19835918

13. Electrochemical detection of β-1,3-glucanase gene from transgenic capsicums using asymmetric PCR generated by a detecting probe and an anchoring probe

Author:
Wu, Guofan; Wang, Zhihua; Zhang, Huini; Yang, Ning; Du, Jie; Lu, Xiaoquan; Kang, Jingwan
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 1-8
ISSN:
0168-1656
Subject:
Capsicum; transgenic plants; beta-glucanase; transgenes; polymerase chain reaction; DNA probes; electrochemistry; electrodes; recombinant DNA; genetic vectors
Abstract:
... A design for recognition of β-1,3-glucanase gene (Glu) specific sequence based on probe extension was described. The detecting probe DNA and the anchoring prober were hybridized with the same target DNA firstly, then the probes were extended by DNA polymerase reaction. After that the double strand DNA was denatured, and the extended detecting probe was immobilized on a glassy carbon electrode via ...
DOI:
10.1016/j.jbiotec.2009.09.013

14. Enhanced expression and activity yields of Clostridium thermocellum xylanases without non-catalytic domains

Author:
Sajjad, Muhammad; Khan, M. Imran M.; Akbar, Nadeem S.; Ahmad, Sajjad; Ali, Imran; Akhtar, M. Waheed
Source:
Journal of biotechnology 2010 v.145 no.1 pp. 38-42
ISSN:
0168-1656
Subject:
Clostridium thermocellum; xylanases; genes; gene expression; messenger RNA; enzyme activity; molecular cloning; heat stability
Abstract:
... Two major xylanase components, XynC and XynZ from the anaerobic thermophilic bacterium, Clostridium thermocellum cellulosome were cloned and expressed with and without non-catalytic domains in E. coli. Two constructs of XynC, one with its cellulose binding domain and the catalytic domain (pXynC-BC) and the other with only the catalytic domain (pXynC-C) were produced. For XynZ the constructs produc ...
DOI:
10.1016/j.jbiotec.2009.10.013
PubMed:
19861138