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- Sergeant, Susan; Johnson, W. Thomas
- Journal of cellular physiology 1995 v.163 no.3 pp. 477
- iron; copper; nutrient availability; nutrient requirements; human cell lines; leukemia; cell proliferation; cell differentiation; retinoic acid; enzyme activity; cytochrome-c oxidase; superoxide dismutase; humans
- ... Trace mineral deficiencies tend to have profound effects on the integrity of formed blood elements. Anemia and neutropenia are commonly seen in copper (Cu) deficiency. We therefore developed a serum-free medium to examine the trace mineral requirements, in particular iron and Cu, for proliferation and retinoic acid (RA)-induced differentiation of HL-60 cells. This defined medium (DFM) was composed ...