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- Rasheed, Awais; Jin, Hui; Xiao, Yonggui; Zhang, Yan; Hao, Yuanfeng; Zhang, Yong; Hickey, Lee T.; Morgounov, Alexey I.; Xia, Xianchun; He, Zhonghu
- Journal of cereal science 2019 v.85 pp. 305-309
- chromosome translocation, etc ; alleles; breadmaking quality; breeding; chromosomes; gene overexpression; genetic markers; genotype; germplasm; germplasm screening; glutenins; hexaploidy; landraces; polymerase chain reaction; wheat; Show all 15 Subjects
- ... We developed and validated high-throughput Kompetitive Allele-Specific PCR (KASP) assays for key genes underpinning bread-making quality, including the wbm gene on chromosome 7AL and over-expressed glutenin Bx7OE (Glu-B1al) gene. Additionally, we used pre-existing KASP assay for Sec1 (1B.1R translocation) gene on chromosome 1B. The newly developed KASP assays were compared with gel-based markers f ...