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You searched for: Journal name Journal of chromatography Remove constraint Journal name: Journal of chromatography Publication year rev 7980-2020 Remove constraint Publication year rev: 7980-2020 Source 2020 v.1143 Remove constraint Source: 2020 v.1143
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1. Development and validation of a GC-FID method for the analysis of short chain fatty acids in rat and human faeces and in fermentation fluids

Author:
Serena Scortichini; Maria Chiara Boarelli; Stefania Silvi; Dennis Fiorini
Source:
Journal of chromatography 2020 v.1143 pp. 121972
ISSN:
1570-0232
Subject:
acetic acid; acidification; chemical species; derivatization; ethyl ether; feces; fermentation; flame ionization; hexanoic acid; humans; intestinal microorganisms; metabolites; mice; polyethylene glycol; propionic acid; quantitative analysis; rats
Abstract:
... Short-chain fatty acids (SCFAs) are gut microbiota metabolites recognized for their beneficial effects on the host organism. In this study, a simple and rapid sample preparation method combined to SCFAs analysis by direct injection and gas chromatography coupled with flame ionization detection (GC-FID), for the determination and quantification of eight SCFAs (acetic, propionic, i-butyric, butyric, ...
DOI:
10.1016/j.jchromb.2020.121972

2. Development of HPLC method for estimation of glyoxylic acid after pre-column fluorescence derivatization approach based on thiazine derivative formation: A new application in healthy and cardiovascular patients’ sera

Author:
Marwa F.B. Ali; Naoya Kishikawa; Naotaka Kuroda
Source:
Journal of chromatography 2020 v.1143 pp. 122054
ISSN:
1570-0232
Subject:
advanced glycation end products; biochemical pathways; derivatization; detection limit; fluorescence; glyoxylic acid; high performance liquid chromatography; mammals; metabolites; monitoring; pathogenesis; patients; thiols; wavelengths
Abstract:
... Glyoxylic acid (GA) is the intermediate metabolite in various mammalian metabolic pathways. GA showed high reactivity towards formation of advanced glycation end-products (AGEs); the main cause of pathogenesis and complications of many diseases. The presented study aimed to detect GA in healthy and cardiovascular patients’ (CV) sera; however analysis of GA in biological fluid is a challenge and re ...
DOI:
10.1016/j.jchromb.2020.122054

3. Metabolic profiling by reversed-phase/ion-exchange mass spectrometry

Author:
Anthony Le; Justin Mak; Tina M. Cowan
Source:
Journal of chromatography 2020 v.1143 pp. 122072
ISSN:
1570-0232
Subject:
bile acids; data collection; derivatization; hydrophilic interaction chromatography; ion exchange; ionization; isoleucine; isomers; leucine; mass spectrometry; metabolites; metabolomics; methylmalonic acid; non-polar compounds; nucleosides; principal component analysis; steroid hormones; succinic acid; urine; vitamins
Abstract:
... Metabolic profiling is commonly achieved by mass spectrometry (MS) following reversed-phase (RP) and hydrophilic interaction chromatography (HILIC) either performed independently, leading to overlapping datasets, or in a coupled configuration, requiring multiple liquid chromatography (LC) systems. To overcome these limitations, we developed a single, 20-minute chromatographic method using an in-li ...
DOI:
10.1016/j.jchromb.2020.122072

4. Quantitative analysis of pesticide residues in tea by gas chromatography–tandem mass spectrometry with atmospheric pressure chemical ionization

Author:
Shizuka Saito-Shida; Mari Nagata; Satoru Nemoto; Hiroshi Akiyama
Source:
Journal of chromatography 2020 v.1143 pp. 122057
ISSN:
1570-0232
Subject:
HCH (pesticide); aldrin; atmospheric pressure; carbon; chlorine; gas chromatography-mass spectrometry; hydrogen; ionization; ions; lindane; monitoring; multiresidue analysis; pesticide residues; pyrethrins; quantitative analysis; standard deviation; tandem mass spectrometry; tea
Abstract:
... In this study, gas chromatography–tandem mass spectrometry (GC–MS/MS) using an atmospheric pressure chemical ionization (APCI) source was applied for the quantitative analysis of pesticide residues in tea. To determine the optimum ionization conditions for multiresidue analysis, the full-scan mass spectra and peak intensities of pesticides were compared in the presence and absence of water as a mo ...
DOI:
10.1016/j.jchromb.2020.122057

5. A green and low-cost method employing switchable hydrophilicity solvent for the simultaneous determination of antidepressants in human urine by gas chromatography - mass spectrometry detection

Author:
Anderson Luiz Oenning; Letícia Birk; Sarah Eller; Tiago Franco de Oliveira; Josias Merib; Eduardo Carasek
Source:
Journal of chromatography 2020 v.1143 pp. 122069
ISSN:
1570-0232
Subject:
antidepressants; aromatic amines; cost effectiveness; detection limit; diphenylamine; gas chromatography-mass spectrometry; humans; hydrophilicity; liquid-phase microextraction; sodium hydroxide; solvents; toxicology; urine; Brazil
Abstract:
... In this study, the use of switchable hydrophilicity solvent with a simple and low-cost lab-made device for the extraction procedure in homogeneous liquid-liquid microextraction is proposed for the first time in the determination of antidepressants in human urine. The antidepressants studied consisted of fluoxetine, amitriptyline, nortriptyline, imipramine, desipramine and sertraline. The optimizat ...
DOI:
10.1016/j.jchromb.2020.122069

6. A rapid, simple and highly sensitive UPLC-MS/MS method for quantitation of pimavanserin in plasma and tissues: Application to pharmacokinetics and brain uptake studies in mice

Author:
Essam Ezzeldin; Muzaffar Iqbal; Yousif A. Asiri; Azza A. Ali; Toqa El-Nahhas
Source:
Journal of chromatography 2020 v.1143 pp. 122015
ISSN:
1570-0232
Subject:
Parkinson disease; acetonitrile; ammonium acetate; blood plasma; blood-brain barrier; brain; chromatography; drugs; enzymes; formic acid; liquid-liquid extraction; methyl ethers; mice; monitoring; pharmacokinetics; solvents; tandem mass spectrometry; tissues
Abstract:
... Pimavanserin is a new drug approved by the FDA for Parkinson's disease psychosis and other neurological disorders such as Alzheimer's disease. In this study, we developed a UPLC-MS/MS method to quantify pimavanserin disposition in the brain and its pharmacokinetics in mice. Vilazodone was used as the internal standard. Pimavanserin and IS were extracted by liquid-liquid extraction using tert-butyl ...
DOI:
10.1016/j.jchromb.2020.122015

7. Biofluid sampler: A new gateway for mail-in-analysis of whole blood samples

Author:
M. Locatelli; A. Tartaglia; F. D'Ambrosio; P. Ramundo; H.I. Ulusoy; K.G. Furton; A. Kabir
Source:
Journal of chromatography 2020 v.1143 pp. 122055
ISSN:
1570-0232
Subject:
acetonitrile; adsorption; ambient temperature; blood sampling; blood serum; cellulose; chromatography; diclofenac; drying; fabrics; hematocrit; humans; ketoprofen; methanol; pH; quantitative analysis; samplers; sorbents; Italy; United States
Abstract:
... The current study reports the development of a novel biofluid sampler (BFS) which is capable of sampling and sample preparation of whole blood without converting it into plasma or serum. The sampler can retain a whole blood sample from 10 to 1000 µL. Although the device shares the same working principle of dried blood spot (DBS) cards, it eliminates most of the technological shortcomings of DBS ca ...
DOI:
10.1016/j.jchromb.2020.122055

8. Immobilized metal affinity chromatography matrix modified by poly (ethylene glycol) methyl ether for purification of angiotensin I-converting enzyme inhibitory peptide from casein hydrolysate

Author:
Pengru Liu; Xiongdiao Lan; Muhammad Yaseen; Kungang Chai; Liqin Zhou; Jianhua Sun; Ping Lan; Zhangfa Tong; Dankui Liao; Lixia Sun
Source:
Journal of chromatography 2020 v.1143 pp. 122042
ISSN:
1570-0232
Subject:
adsorption; affinity chromatography; casein hydrolysates; dietary supplements; enzyme inhibition; functional foods; hypertension; in vitro digestion; ingredients; inhibitory concentration 50; magnetism; methyl ethers; peptides; peptidyl-dipeptidase A; polyethylene glycol
Abstract:
... Purification of small bioactive peptides from complex biological samples is a difficult task due to the interference of concentrated large biomolecules. In this study, a magnetic immobilized metal affinity chromatography matrix modified by poly (ethylene glycol) methyl ether (IMACM@mPEG) was prepared and applied for the rapid purification of angiotensin I-converting enzyme (ACE) inhibitory peptide ...
DOI:
10.1016/j.jchromb.2020.122042