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You searched for: Journal name Journal of chromatography Remove constraint Journal name: Journal of chromatography Subject term metabolites Remove constraint Subject term: metabolites
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1. 3F-α-pyrrolydinovalerophenone (3F-α-PVP) in vitro human metabolism: Multiple in silico predictions to assist in LC-HRMS/MS analysis and targeted/untargeted data mining

Author:
Jeremy Carlier; Diletta Berardinelli; Eva Montanari; Ascanio Sirignano; Annagiulia Di Trana; Francesco P. Busardò
Source:
Journal of chromatography 2022 v.1193 pp. 123162
ISSN:
1570-0232
Subject:
biochemical pathways; biomarkers; cannabinoids; chromatography; computer simulation; forensic sciences; hepatocytes; humans; hydroxylation; metabolism; metabolites; pentanols; poisoning; toxicology; Sweden
Abstract:
... Synthetic cathinones (SCs) constitute a heterogenous class of new psychoactive substances (NPS), structurally related to cathinone. SCs represent the widest NPS class, second to synthetic cannabinoids, accounting for approximately 160 different analogues with substitution at the phenyl group, the amine group, or the alkyl chain. In 2020, α-pyrrolidonophenone analogues were the most trafficked SCs, ...
DOI:
10.1016/j.jchromb.2022.123162

2. A clinically validated method to separate and quantify underivatized acylcarnitines and carnitine metabolic intermediates using mixed-mode chromatography with tandem mass spectrometry

Author:
Carolina Luna; Chandler Griffin; Marcus J. Miller
Source:
Journal of chromatography 2022 v.1663 pp. 462749
ISSN:
0021-9673
Subject:
amino acid metabolism; beta oxidation; biomarkers; carnitine; chemical species; derivatization; humans; liquid chromatography; metabolites; mitochondria; patients; tandem mass spectrometry
Abstract:
... Acylcarnitines are intermediate metabolites of the mitochondria that serve as biomarkers for inherited disorders of fatty acid oxidation and amino acid metabolism. The prevailing clinical method used to quantify acylcarnitines involves flow-injection tandem mass spectrometry, an approach with a number of limitations; foremost the inability to separate and therefore distinguish key isobaric acylcar ...
DOI:
10.1016/j.chroma.2021.462749

3. A multi-dimensional liquid chromatography/high-resolution mass spectrometry approach combined with computational data processing for the comprehensive characterization of the multicomponents from Cuscuta chinensis

Author:
Miao Wang; Xiao-yan Xu; Hong-da Wang; Hui-min Wang; Mei-yu Liu; Wan-di Hu; Bo-xue Chen; Mei-ting Jiang; Jing Qi; Xiao-hang Li; Wen-zhi Yang; Xiu-mei Gao
Source:
Journal of chromatography 2022 v.1675 pp. 463162
ISSN:
0021-9673
Subject:
Cuscuta; data collection; databases; hydrophilic interaction chromatography; isomerization; liquids; mass spectrometry; metabolites; metabolomics; prediction; quality control; reversed-phase liquid chromatography
Abstract:
... Challenges encountered in plant metabolites characterization by liquid chromatography/mass spectrometry can arise from the insufficient chromatography separation, the lack of specific database, and low reliability of identification because of the ubiquitous isomerism. Herein, we present an integral approach, by combining comprehensive off-line two-dimensional liquid chromatography/ion mobility-qua ...
DOI:
10.1016/j.chroma.2022.463162

4. A quantitative metabolomics assay targeting 14 intracellular metabolites associated with the methionine transsulfuration pathway using LC–MS/MS in breast cancer cells

Author:
Zixin Jiao; Zhenyao Lu; Ying Peng; Chen Xu; Yunge Lou; Guangji Wang; Jiye Aa; Yue Zhang
Source:
Journal of chromatography 2022 pp. 123314
ISSN:
1570-0232
Subject:
S-adenosylmethionine; betaine; breast neoplasms; cystathionine; cysteine; cystine; glutathione; homeostasis; homocysteine; liquid chromatography; metabolism; metabolites; metabolomics; methylation; quantitative analysis; serine; tandem mass spectrometry; tetrahydrofolic acid
Abstract:
... The methionine transsulfuration pathway plays an important role in some fundamental biological processes, such as redox and methylation reactions. However, quantitative analysis of the majority of intracellular metabolites is rather challenging. In this study, we developed a simple, fast and reliable method using liquid chromatography–tandem mass spectrometry (LC–MS/MS) for the simultaneous detect ...
DOI:
10.1016/j.jchromb.2022.123314

5. A rapid HPLC–MS/MS method for the simultaneous determination of luteolin, resveratrol and their metabolites in rat plasma and its application to pharmacokinetic interaction studies

Author:
Wenying Wu; Kexin Li; Chen Zhao; Xiaohua Ran; Yu Zhang; Tianhong Zhang
Source:
Journal of chromatography 2022 v.1191 pp. 123118
ISSN:
1570-0232
Subject:
ammonium acetate; bioavailability; chemical species; daidzein; electrospray ionization mass spectrometry; formic acid; liver; luteolin; metabolites; methanol; naringenin; pharmacokinetics; polyphenols; rats; resveratrol; therapeutics; uridine
Abstract:
... Both luteolin (LUT) and resveratrol (RES) are natural polyphenols that exert therapeutic effects on liver injuries. Extensive glucuronidation by uridine diphosphate-glucuronosyltransferases 1As (UGT1As) results in poor bioavailability of LUT, which limits its clinical application. As an inhibitor of UGT1A1 and UGT1A9, RES may affect the bioavailability of LUT. The purpose of this study was to deve ...
DOI:
10.1016/j.jchromb.2022.123118

6. A rapid and sensitive method for the quantification of dopamine and serotonin metabolites in cerebrospinal fluid based on UHPLC with fluorescence detection

Author:
Ayoub Boulghobra; Myriam Bonose; Isabelle Billault; Antoine Pallandre
Source:
Journal of chromatography 2022 v.1200 pp. 123264
ISSN:
1570-0232
Subject:
5-hydroxytryptophan; biomarkers; cerebrospinal fluid; dopamine; electrochemistry; fluorescence; homovanillic acid; metabolism; metabolites; serotonin; ultra-performance liquid chromatography
Abstract:
... Inborn errors of dopamine and serotonin metabolism are diseases caused by deficiencies in enzymes belonging to metabolic pathways. The specific diagnosis of these inborn illnesses is based on the identification and quantification of biomarkers in cerebrospinal fluid (CSF), especially: 5-hydroxy-tryptophane (5-HTP), 5-hydroxy-indol-acetic acid (5-HIAA), 3-ortho-methyl-DOPA (3-OMD), homovanillic aci ...
DOI:
10.1016/j.jchromb.2022.123264

7. A ‘shape-orientated’ algorithm employing an adapted Marr wavelet and shape matching index improves the performance of continuous wavelet transform for chromatographic peak detection and quantification

Author:
Caihong Bai; Suyun Xu; Jingyi Tang; Yuxi Zhang; Jiahui Yang; Kaifeng Hu
Source:
Journal of chromatography 2022 v.1673 pp. 463086
ISSN:
0021-9673
Subject:
algorithms; blood serum; humans; metabolites; metabolomics; wavelet
Abstract:
... A new ‘shape-orientated’ continuous wavelet transform (CWT)-based algorithm employing an adapted Marr wavelet (AMW) with a shape matching index (SMI), defined as peak height normalized wavelet coefficient (C′max/H) for feature filtering, was developed for chromatographic peak detection and quantification. Exploiting the chromatographic profile of a candidate peak, AMW-SMI algorithm emphasizes more ...
DOI:
10.1016/j.chroma.2022.463086

8. Active microbial metabolites study on antitussive and expectorant effects and metabolic mechanisms of platycosides fraction of Platycodonis Radix

Author:
Chi Zhang; Xinhong Wang; Yuanhan Zhong; Li Zhou; Jian Liang; Jinxiang Zeng; Lifen Zhou; En Yuan; Jixiao Zhu; Chong-Zhi Wang; Chun-Su Yuan
Source:
Journal of chromatography 2022 v.1195 pp. 123171
ISSN:
1570-0232
Subject:
arachidonic acid; biotransformation; chromatography; glycerophospholipids; intestinal microorganisms; linoleic acid; metabolites; metabolomics
Abstract:
... A new method involving gut microbiota biotransformation, spectrum-effect relationship analysis and metabolomics analysis was developed to study the antitussive and expectorant microbial metabolites of platycosides fraction (MPFs) of Platycodonis Radix. Furthermore, their possible metabolic mechanisms were studied for the first time. The findings showed that the antitussive and expectorant effects ...
DOI:
10.1016/j.jchromb.2022.123171

9. An UHPLC/LC-MS illustrated the dynamic profiling of balanophorin B, gallic acid, and 4-hydroxycinnamic acid in rat as 3 molecular entities from Balanophora simaoensis

Author:
Xiangting Xu; Rujun Tao; Kexian Li; Wei Wang
Source:
Journal of chromatography 2022 v.1190 pp. 123103
ISSN:
1570-0232
Subject:
Balanophora; bile; biotransformation; calibration; ethanol; excreta; gallic acid; hydroxybenzaldehyde; liver; mass spectrometry; metabolites; oral administration; p-coumaric acid; pharmacodynamics; rats
Abstract:
... An UHPLC/LC-MS was founded to detect balanophorin B (B), gallic acid (GA), 4-hydroxycinnamic acid (HC), and their in vivo profiling in rats, after oral administration of the ethanol extract of Balanophora simaoensis S. Y. Chang et Tam. The in vivo dynamic existence of 3 molecular entities in rats and the multistep biotransformation of GA were elucidated by their sensitive mass spectrometry respons ...
DOI:
10.1016/j.jchromb.2022.123103

10. An integrated chemical characterization based on FT-NIR, GC–MS and LC-MS for the comparative metabolite profiling of wild and cultivated agarwood

Author:
Cheng Yao; Luming Qi; Furong Zhong; Na Li; Yuntong Ma
Source:
Journal of chromatography 2022 v.1188 pp. 123056
ISSN:
1570-0232
Subject:
Oriental traditional medicine; aromatic compounds; biochemical polymorphism; chemical composition; gas chromatography-mass spectrometry; metabolites; multivariate analysis; principal component analysis; sesquiterpenoids; tandem mass spectrometry; ultra-performance liquid chromatography
Abstract:
... Agarwood is a well-known and precious traditional Chinese medicine, has been widely applied as drugs and spices for century. The large demand for this material has deeply stimulated the emergence of numerous cultivated products. However, it is difficult to distinguish wild agarwood from cultivated agarwood, and the chemical composition difference between them is not clear. In this study, an integr ...
DOI:
10.1016/j.jchromb.2021.123056

11. An off-line three-dimensional liquid chromatography/Q-Orbitrap mass spectrometry approach enabling the discovery of 1561 potentially unknown ginsenosides from the flower buds of Panax ginseng, Panax quinquefolius and Panax notoginseng

Author:
Li Jia; Hongda Wang; Xiaoyan Xu; Huimin Wang; Xue Li; Ying Hu; Boxue Chen; Meiyu Liu; Xiumei Gao; Huifang Li; Dean Guo; Wenzhi Yang
Source:
Journal of chromatography 2022 v.1675 pp. 463177
ISSN:
0021-9673
Subject:
Panax ginseng; Panax notoginseng; Panax quinquefolius; flowers; ginsenosides; hydrophilic interaction chromatography; hydrophilicity; hydrophobic bonding; ion exchange chromatography; liquids; mass spectrometry; metabolites; molecular weight; reversed-phase liquid chromatography; spectrometers
Abstract:
... To comprehensively elucidate the herbal metabolites is crucial in natural products research to discover new lead compounds. Ginsenosides are an important class of bioactive components from the Panax plants exerting the significant tonifying effects. However, to identify new ginsenosides by the conventional strategies trends to be more and more difficult because of the large spans of acid-base prop ...
DOI:
10.1016/j.chroma.2022.463177

12. An online stepwise background subtraction-based ultra-high pressure liquid chromatography quadrupole time of flight tandem mass spectrometry dynamic detection integrated with metabolic molecular network strategy for intelligent characterization of the absorbed chemical-fingerprint of QiangHuoShengShi decoction in vivo

Author:
Jiading Guo; Ye Shang; Xiaohua Yang; Jin Li; Jun He; Xiumei Gao; Yanxu Chang
Source:
Journal of chromatography 2022 v.1675 pp. 463172
ISSN:
0021-9673
Subject:
Oriental traditional medicine; coumarins; data collection; flavonoids; liquid chromatography; metabolites; oral administration; phthalides; prediction; rats; saponins; tandem mass spectrometry
Abstract:
... QiangHuoShengShi decoction (QHSS) was an ancient and classical traditional Chinese medicine (TCM) prescription. In the previous study, its phytochemical fingerprint had been comprehensively characterized. However, no reports were available on its absorbed prototypes and the related metabolites in rat plasma samples. In this study, an intelligent and innovate analysis strategy was built for charact ...
DOI:
10.1016/j.chroma.2022.463172

13. Analysis of plasma free amino acids in diabetic rat and the intervention of Ginkgo biloba leaves extract using hydrophilic interaction liquid chromatography coupled with tandem mass-spectrometry

Author:
Fan Zhang; Ding-xiang Li; Dong-yu Lu; Yi-fan Lu; Ran Zhang; Lin-lin Zhao; Shuai Ji; Meng-Zhe Guo; Yan Du; Dao-Quan Tang
Source:
Journal of chromatography 2022 v.1196 pp. 123230
ISSN:
1570-0232
Subject:
Ginkgo biloba; alanine; diabetes mellitus; glutamic acid; hydrophilic interaction chromatography; isoleucine; leucine; lysine; metabolism; metabolites; rats; streptozotocin; tandem mass spectrometry; therapeutics; tyrosine
Abstract:
... Amino acids (AAs) are important metabolites that are related with diabetes. However, their roles in the initiation and development of diabetes mellitus (DM), especially in the treatment of Ginkgo biloba leaves extract (GBE) have not been fully explored. Thus, we investigated the roles that AAs played in the progression and GBE supplementation of DM rat induced by streptozotocin. The rats were rand ...
DOI:
10.1016/j.jchromb.2022.123230

14. Analysis of sodium 2-mercaptoethane sulfonate in rat plasma using high performance liquid chromatography tandem-mass spectrometry

Author:
Abigail B. Donkor; Carl W. White; Heidi J. Nick; Brian A. Logue
Source:
Journal of chromatography 2022 v.1189 pp. 123088
ISSN:
1570-0232
Subject:
acrolein; blood proteins; centrifugation; colorimetry; cyclophosphamide; detection limit; electrochemistry; evaporation; fluorescence; high performance liquid chromatography; metabolites; rats; sodium; standard deviation; sulfonates; tandem mass spectrometry; therapeutics; toxicity
Abstract:
... Sodium 2-mercaptoethane sulfonate (MESNA) is a thiol-containing compound that has proven to be effective in inactivating acrolein, the toxic metabolite of some anti-cancer drugs (e.g., cyclophosphamide and ifosphamide). Also, it scavenges free radicals which cause numerous disorders by attacking biological molecules. Current methods available to analyze MESNA in biological matrices include colorim ...
DOI:
10.1016/j.jchromb.2021.123088

15. Application of a hybrid zwitterionic hydrophilic interaction liquid chromatography column in metabolic profiling studies

Author:
Artemis Lioupi; Christina Virgiliou; Thomas H. Walter; Kerri M. Smith; Paul Rainville; Ian D Wilson; Georgios Theodoridis; Helen G Gika
Source:
Journal of chromatography 2022 v.1672 pp. 463013
ISSN:
0021-9673
Subject:
hydrophilic interaction chromatography; hydrophilicity; liver; metabolites; mice; models; phenotype; sulfobetaine; zwitterions
Abstract:
... Metabolic phenotyping studies using mouse liver extracts as a model, performed on a novel zwitterionic HILIC UHPLC column, which is based on ethylene-bridged hybrid organic/inorganic particles bonded with sulfobetaine groups and packed into column hardware modified with hybrid surface technology are reported. Initially the chromatographic performance was evaluated under different mobile phase cond ...
DOI:
10.1016/j.chroma.2022.463013

16. Black raspberry extract shifted gut microbe diversity and their metabolic landscape in a human colonic model

Author:
Shiqi Zhang; Mengyang Xu; Xiaowei Sun; Xuyu Liu; Fouad Choueiry; Rui Xu; Haifei Shi; Jiangjiang Zhu
Source:
Journal of chromatography 2022 v.1188 pp. 123027
ISSN:
1570-0232
Subject:
Enterococcus; Rubus occidentalis; anthocyanins; arginine; biosynthesis; colon; dysbiosis; human health; humans; intestinal microorganisms; irritable bowel syndrome; liquid chromatography; lysine; mass spectrometry; metabolites; metabolomics; models; obesity; pH; proline; ribosomal DNA; temperature
Abstract:
... Human gut microbiota is critical for human health, as their dysbiosis could lead to various diseases such as irritable bowel syndrome and obesity. Black raspberry (BRB) has been increasingly studied recently for its impact on gut microbiota as a rich source of phytochemicals (e.g., anthocyanin). To investigate the effect of BRB extract on the gut microbiota composition and their metabolism, an in- ...
DOI:
10.1016/j.jchromb.2021.123027

17. Combined 16S rRNA gene sequencing and metabolomics to investigate the protective effects of Wu-tou decoction on rheumatoid arthritis in rats

Author:
Xiaoxu Cheng; Zifeng Pi; Zhong Zheng; Shu Liu; Fengrui Song; Zhiqiang Liu
Source:
Journal of chromatography 2022 v.1199 pp. 123249
ISSN:
1570-0232
Subject:
Bacteroides; Oriental traditional medicine; Prevotella; acetic acid; bile; blood serum; butyric acid; cholic acid; chromatography; dysbiosis; genes; indoles; inflammation; intestinal microorganisms; intestines; metabolites; metabolomics; propionic acid; rheumatoid arthritis; therapeutics; tryptophan
Abstract:
... Wu-tou decoction (WTD) is a traditional Chinese medicine (TCM) formula which has been used for treating rheumatoid arthritis (RA) for a thousand years. However, the underlying mechanism of WTD in treating RA is still unclear. In recent years, more and more attention has been paid to the role of gut microbiota and microbiota-derived metabolites in the treatment of RA. Hence, this study aims to inve ...
DOI:
10.1016/j.jchromb.2022.123249

18. Combining high-speed countercurrent chromatography three-phase solvent system with electrospray ionization-mass spectrometry and nuclear magnetic resonance to profile the unconventional food plant Syzygium malaccense

Author:
Aline Gomes Lopes; Ricardo Moreira Borges; Stefan Kuhn; Rafael Garrett; Fernanda das Neves Costa
Source:
Journal of chromatography 2022 pp. 463211
ISSN:
0021-9673
Subject:
Syzygium malaccense; acetonitrile; chemical composition; countercurrent chromatography; electrospray ionization mass spectrometry; ethyl acetate; flavonoids; food plants; fractionation; hexane; leaf extracts; metabolites; nuclear magnetic resonance spectroscopy; solvents; trees; Brazil; Malaysia
Abstract:
... Syzygium malaccense (L.) Merr. & L.M. Perry is a native tree to Malaysia, but also occurs in other tropical regions of the world, including Brazil. The increasing interest in the consumption of its leaves motivated the investigation of compounds of the plant. Metabolite profiling of S. malaccense leaves was achieved by high-speed countercurrent chromatography (HSCCC) fractionation coupled off-line ...
DOI:
10.1016/j.chroma.2022.463211

19. Comprehensive metabolic study of nicotine in equine plasma and urine using liquid chromatography/high-resolution mass spectrometry for the identification of unique biomarkers for doping control

Author:
Hideaki Ishii; Gary Ngai-Wa Leung; Shozo Yamashita; Shun-ichi Nagata; Asuka Kushiro; Satoshi Sakai; Kota Toju; Jun Okada; Kazumi Kawasaki; Kanichi Kusano; Isao Kijima-Suda
Source:
Journal of chromatography 2022 v.1190 pp. 123100
ISSN:
1570-0232
Subject:
Thoroughbred; biomarkers; chemical species; electrospray ionization mass spectrometry; liquid chromatography; liquids; metabolic studies; metabolism; metabolites; nicotine; risk; smoke; urine
Abstract:
... Nicotine is classified as a stimulant, and its use is banned in horse racing and equestrian sports by the International Federation of Horseracing Authorities and the Fédération Équestre Internationale, respectively. Because nicotine is a major alkaloid of tobacco leaves, there is a potential risk that doping control samples may be contaminated by tobacco cigarettes or smoke during sample collectio ...
DOI:
10.1016/j.jchromb.2022.123100

20. Current state-of-the-art of separation methods used in LC-MS based metabolomics and lipidomics

Author:
Eva-Maria Harrieder; Fleming Kretschmer; Sebastian Böcker; Michael Witting
Source:
Journal of chromatography 2022 v.1188 pp. 123069
ISSN:
1570-0232
Subject:
hydrophilic interaction chromatography; lipidomics; lipids; mass spectrometry; metabolites; metadata
Abstract:
... Metabolomics deals with the large-scale analysis of metabolites, belonging to numerous compound classes and showing an extremely high chemical diversity and complexity. Lipidomics, being a subcategory of metabolomics, analyzes the cellular lipid species. Both require state-of-the-art analytical methods capable of accessing the underlying chemical complexity. One of the major techniques used for th ...
DOI:
10.1016/j.jchromb.2021.123069

21. Cyclophosphamide, hydroxycyclophosphamide and carboxyethyl phosphoramide mustard quantification with liquid chromatography mass spectrometry in a single run human plasma samples: A rapid and sensitive method development

Author:
Kripa Shanker Kasudhan; Ajay Patial; Nancy Mehra; Savita Verma Attri; Pankaj Malhotra; Smita Pattanaik; Deepesh Lad; Amol N. Patil
Source:
Journal of chromatography 2022 v.1198 pp. 123228
ISSN:
1570-0232
Subject:
acetonitrile; ammonium acetate; cell transplantation; cyclophosphamide; formic acid; humans; leukemia; liquid chromatography; metabolites; particle size; pharmacokinetics; quality control; tandem mass spectrometry
Abstract:
... A novel method for simultaneous quantification of cyclophosphamide along with its two major metabolites namely 4-hydroxycyclophosphamide (HCy) and carboxyethyl phosphoramide mustard (CEPM) in a single sample run was demonstrated in the present study. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) instrument was used for analysis. Semicarbazide was used as a stabilizing agent for HCy w ...
DOI:
10.1016/j.jchromb.2022.123228

22. Deep learning for retention time prediction in reversed-phase liquid chromatography

Author:
Elizaveta S. Fedorova; Dmitriy D. Matyushin; Ivan V. Plyushchenko; Andrey N. Stavrianidi; Aleksey K. Buryak
Source:
Journal of chromatography 2022 v.1664 pp. 462792
ISSN:
0021-9673
Subject:
data collection; databases; high performance liquid chromatography; mass spectrometry; metabolites; neural networks; prediction; reversed-phase liquid chromatography
Abstract:
... Retention time prediction in high-performance liquid chromatography (HPLC) is the subject of many studies since it can improve the identification of unknown molecules in untargeted profiling using HPLC coupled with high-resolution mass spectrometry. Lots of approaches were developed for retention time prediction in liquid chromatography for a different number of molecules considering various molec ...
DOI:
10.1016/j.chroma.2021.462792

23. Design and synthesis of amphiphilic carboxyl-functionalized magnetic polymer microspheres for fast determination of paraquat and its four metabolites in human urine samples prior to ultra-high performance liquid chromatography-high resolution mass spectrometry

Author:
Shengdong Pan; Zhiping Huang; Chunqing Shang; Li Wang; Qiaoli Qiu; Zemin Xu; Dandan Zhang
Source:
Journal of chromatography 2022 v.1670 pp. 462998
ISSN:
0021-9673
Subject:
Fourier transform infrared spectroscopy; X-ray photoelectron spectroscopy; adsorbents; adsorption; electrostatic interactions; humans; hydrophilicity; liquid chromatography; magnetism; mass spectrometry; metabolites; microparticles; paraquat; polymers; prototypes; solid phase extraction; thermogravimetry; transmission electron microscopes; urine
Abstract:
... In cases of low concentration paraquat (PQ) poisoning, as the disease progresses over a long period of time, the prototype PQ may not be detected in the urine, which has a significant negative impact on the precise treatment of the poisoning. But PQ poisoning can also be confirmed by the detection of specific metabolites of PQ in the urine samples. In the present study, core-shell amphiphilic carb ...
DOI:
10.1016/j.chroma.2022.462998

24. Determination of six thyroid hormones in dog brain and liver using acidic extraction, mixed-mode cleanup, and liquid chromatography–tandem mass spectrometry

Author:
Kohki Takaguchi; Sumika Ono; Rumi Tanoue; Tatsuya Kunisue; Shinsuke Tanabe; Kei Nomiyama
Source:
Journal of chromatography 2022 v.1661 pp. 462686
ISSN:
0021-9673
Subject:
Canis lupus; L-thyroxine; acetone; brain; cation exchange; dogs; formic acid; homeostasis; iodide peroxidase; liquid chromatography; liver; metabolism; metabolites; polychlorinated biphenyls; tandem mass spectrometry; thyroid gland
Abstract:
... Thyroid hormones (THs) play a critical role in the regulation of biological processes, such as growth, metabolism, and development, in various animal species. Prohormone L-thyroxine (T4) is secreted from the thyroid gland and carried to peripheral tissues. T4 is then biotransformed to several metabolites which play different roles, mainly by iodothyronine deiodinases. Determination of deiodinated ...
DOI:
10.1016/j.chroma.2021.462686

25. Development and validation of HPLC-DAD and LC-(ESI)/MS methods for the determination of sulfasalazine, mesalazine and hydrocortisone 21-acetate in tablets and rectal suppositories: In vitro and ex vivo permeability studies

Author:
Vasileios Tsamis; Eleni Tsanaktsidou; Christina Karavasili; Constantinos K. Zacharis; Nikolaos Bouropoulos; Dimitrios G. Fatouros; Catherine K. Markopoulou
Source:
Journal of chromatography 2022 v.1198 pp. 123246
ISSN:
1570-0232
Subject:
cortisol; experimental design; high performance liquid chromatography; intestines; lipids; mass spectrometry; metabolism; metabolites; permeability; sulfamethazine; sulfasalazine; ulcerative colitis
Abstract:
... Controlled-release tablets and rectal suppositories of sulfasalazine (SLF) and hydrocortisone 21-acetate (HA) were prepared as recommended dosage forms for the treatment of acute episodes of ulcerative colitis, in patients who do not respond to monotherapy. A High-Performance Liquid Chromatography (HPLC) Diode-array method with a gradient elution mobile phase was developed to evaluate the producti ...
DOI:
10.1016/j.jchromb.2022.123246

26. Development and validation of a new liquid chromatography-tandem mass spectrometry assay for the simultaneous quantification of afatinib, dacomitinib, osimertinib, and the active metabolites of osimertinib in human serum

Author:
Emi Ishikawa; Yuta Yokoyama; Haruna Chishima; Ouki Kuniyoshi; Itaru Sato; Naoki Nakaya; Hideo Nakajima; Motonori Kimura; Jun Hakamata; Naoya Suehiro; Hideo Nakada; Shinnosuke Ikemura; Aya Jibiki; Hitoshi Kawazoe; Hiroshi Muramatsu; Sayo Suzuki; Tomonori Nakamura
Source:
Journal of chromatography 2022 v.1199 pp. 123245
ISSN:
1570-0232
Subject:
Food and Drug Administration; blood serum; chemical species; drugs; epidermal growth factor; humans; liquid chromatography; lung neoplasms; metabolites; tandem mass spectrometry; therapeutics
Abstract:
... Reports on the therapeutic drug monitoring (TDM) of second- and third-generation epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) in non-small cell lung cancer patients are limited and are required to improve the safety of EGFR-TKI therapy. Some EGFR-TKIs have active metabolites with similar or higher potency compared with the parent compounds; thus, monitoring the parent co ...
DOI:
10.1016/j.jchromb.2022.123245

27. Development and validation of a quantitative method for 15 antiviral drugs in poultry muscle using liquid chromatography coupled to tandem mass spectrometry

Author:
Clément Douillet; Mary Moloney; Melissa Di Rocco; Christopher Elliott; Martin Danaher
Source:
Journal of chromatography 2022 v.1665 pp. 462793
ISSN:
0021-9673
Subject:
antiviral agents; chemical species; chickens; ducks; electrospray ionization mass spectrometry; hydrophilic interaction chromatography; influenza; markets; metabolites; methanol; multiresidue analysis; muscles; poultry meat; quails; quantitative analysis; spectrometers; tandem mass spectrometry
Abstract:
... The objective of this work was to develop a quantitative multi-residue method for analysing antiviral drug residues and their metabolites in poultry meat samples. Antiviral drugs are not licensed for the treatment of influenza in food producing animals. However, there have been some reports indicating their illegal use in poultry. In this study, a method was developed for the analysis of 15 antivi ...
DOI:
10.1016/j.chroma.2021.462793

28. Development and validation of ultra-performance liquid chromatography-tandem mass spectrometric methods for simultaneous and rapid determination of contezolid and its major metabolite M2 in plasma and urine samples and its application to a study in subjects with moderate liver impairment

Author:
Yu Wang; Hailan Wu; Junzhen Wu; Yaxin Fan; Xiaofen Liu; Yi Li; Jiali Hu; Jing Zhang; Beining Guo
Source:
Journal of chromatography 2022 v.1191 pp. 123129
ISSN:
1570-0232
Subject:
acetonitrile; antibacterial properties; automation; chemical species; humans; liquid-liquid extraction; liver; metabolites; methicillin-resistant Staphylococcus aureus; pharmacokinetics; quality control; solid phase extraction; urine
Abstract:
... Contezolid is a novel oxazolidinone antibiotic with good antibacterial activity against gram-positive bacteria including methicillin-resistant Staphylococcus aureus. For the purpose to further characterize the pharmacokinetics of contezolid and its major metabolite M2, accurate and rapid ultra-performance liquid chromatography-tandem mass spectrometric assays (UPLC-MS/MS) were developed and valida ...
DOI:
10.1016/j.jchromb.2022.123129

29. Development of analytical method for the determination of methylphenidate, the analog ethylphenidate and their metabolite ritalinic acid in oral fluid samples by micro-QuEChERS and liquid chromatography–tandem mass spectrometry

Author:
Kauê de Oliveira Chinaglia; Ana Carolina Furiozo Arantes; Kelly Francisco da Cunha; Eduardo Geraldo de Campos; Júlia Martinelli Magalhães Kahl; Leonardo Costalonga Rodrigues; Jose Luiz Costa
Source:
Journal of chromatography 2022 v.1205 pp. 123330
ISSN:
1570-0232
Subject:
calibration; chemical species; forensic sciences; freezers; liquid chromatography; metabolites; music; quantitative analysis; refrigerators; tandem mass spectrometry; toxicology; Brazil
Abstract:
... The aim of this study was to develop a quantitative method for the analysis of methylphenidate, the analog ethylphenidate and their metabolite ritalinic acid in oral fluid, using micro-QuEChERS extraction and liquid chromatography–tandem mass spectrometry (LC–MS/MS). Oral fluid samples were collected with Quantisal™ device, extracted by micro-QuEChERS technique and analyzed by LC–MS/MS. The develo ...
DOI:
10.1016/j.jchromb.2022.123330

30. Effect of the ethyl acetate extract of Sophora flavescens Aiton on diabetic retinopathy based on untargeted retinal metabolomics

Author:
Yun Luo; Kairui Zhao; Zhaocheng Li; Yanping Gao; Minling Lin; Yadi Li; Shumei Wang; Yi Liu; Lei Chen
Source:
Journal of chromatography 2022 v.1198 pp. 123233
ISSN:
1570-0232
Subject:
Oriental traditional medicine; Sophora flavescens; angiogenesis; apoptosis; arginine; bile acids; blindness; blood glucose; blood serum; chalcone; chromatography; diabetic retinopathy; ethyl acetate; glycerophospholipids; heat; lactose; lipopolysaccharides; metabolism; metabolites; metabolomics; neurons; pharmacodynamics; retina; therapeutics; trimethylamine; vision
Abstract:
... Diabetic retinopathy (DR) is the most common microvascular complication of diabetes and a leading cause of vision impairment and blindness, which lacks effective diagnostic measures and therapeutic options. Sophora flavescens Aiton or “Kushen” is a traditional Chinese medicine used since ancient times, either alone or in combination, to clear heat, dampness, and tearing, and to treat ocular diseas ...
DOI:
10.1016/j.jchromb.2022.123233

31. Enantioseparation and quantitative determination of two homologous beta amino acids found in Fabaceae plants

Author:
Nóra Gampe; Lilla Ladocsi; Ida Fejős; Imre Boldizsár; András Darcsi; Szabolcs Béni
Source:
Journal of chromatography 2022 v.1675 pp. 463089
ISSN:
0021-9673
Subject:
capillary electrophoresis; enantiomers; metabolites; pH; quantitative analysis; toxicity; variance
Abstract:
... Non-protein amino acids are important metabolites of the Fabaceae family, possessing valuable biological effects in addition to their toxic properties. We have previously identified two non-protein amino acids homoproline and homopipecolic acid in Ononis species for the first time, and herein the study was extended to investigate further Fabaceae species (O. spinosa, O. arvensis, M. sativa, A. vul ...
DOI:
10.1016/j.chroma.2022.463089

32. Evaluation of different internal standardization approaches for the quantification of melatonin in cell culture samples by multiple heart-cutting two dimensional liquid chromatography tandem mass spectrometry

Author:
Amanda Suárez Fernández; Adriana González Gago; Francisco Artime Naveda; Javier García Calleja; Anna Zawadzka; Zbigniew Czarnocki; Juan Carlos Mayo Barrallo; Rosa M. Sainz Menéndez; Pablo Rodríguez-González; J. Ignacio García Alonso
Source:
Journal of chromatography 2022 v.1663 pp. 462752
ISSN:
0021-9673
Subject:
cell culture; humans; ionization; isotope dilution technique; isotope labeling; liquid chromatography; melatonin; metabolites; prostatic neoplasms; tandem mass spectrometry
Abstract:
... We evaluate here different analytical strategies for the chromatographic separation and determination of N-acetyl-5-methoxytryptamine (MEL) and its oxidative metabolites N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK), N1-acetyl-5-methoxykynuramine (AMK) and cyclic 3-hydroxymelatonin (c3OHM) in cell culture samples. Two dimensional liquid chromatography (2D-LC) in the multiple heart-cutting mode wa ...
DOI:
10.1016/j.chroma.2021.462752

33. Evaluation of multiple reaction monitoring cubed performed by a quadrupole-linear ion trap mass spectrometer for quantitative determination of 6-sulfatoxymelatonin in urine

Author:
Leonid V. Lopukhov; Anna V. Balandina; Lilia S. Nigmatullina; Adelya F. Mullakhmetova; Gulnaz E. Synbulatova; Alexander V. Laikov; Victor L. Lopukhov; Tatiana V. Grigoryeva
Source:
Journal of chromatography 2022 v.1190 pp. 123094
ISSN:
1570-0232
Subject:
blood; liquid chromatography; mass spectrometry; melatonin; metabolites; quantitative analysis; spectrometers; urine
Abstract:
... Liquid chromatography (LC) - mass spectrometry quantitative analysis of substances in biological samples is usually performed in the multiple reaction monitoring (MRM) variant. In complex biological matrices, strong interferences can be observed when using the LC-MRM method. Interference levels can be significantly reduced by using LC - multiple reaction monitoring cubed (MRM³). 6-sulfatoxymelaton ...
DOI:
10.1016/j.jchromb.2021.123094

34. Evaluation of nanospray capillary LC-MS performance for metabolomic analysis in complex biological matrices

Author:
Mahmoud Elhusseiny Mostafa; James P. Grinias; James L. Edwards
Source:
Journal of chromatography 2022 v.1670 pp. 462952
ISSN:
0021-9673
Subject:
amino acids; blood serum; dimerization; metabolites; metabolomics
Abstract:
... LC-MS metabolomic analysis in complex biological matrices may be complicated by degeneracy when using large-bore columns. Degeneracy is the detection of multiple mass spectral peaks from the same analyte due to adduction of salts to the metabolite, dimerization, or loss of neutrals. This introduces interferences to the MS spectra, diminishes quantification, and increases the rate of false identifi ...
DOI:
10.1016/j.chroma.2022.462952

35. Gut microbiota influenced the xenograft MC38 tumor growth potentially through interfering host lipid and amino acid metabolisms, basing on the integrated analysis of microbiome and metabolomics

Author:
Ming-Hui Chen; Jing Zhou; Cheng-Ying Wu; Wei Zhang; Fang Long; Shan-Shan Zhou; Jin-Di Xu; Jie Wu; Ye-Ting Zou; Song-Lin Li; Hong Shen
Source:
Journal of chromatography 2022 v.1192 pp. 123136
ISSN:
1570-0232
Subject:
Bacteroidetes; Firmicutes; Proteobacteria; apoptosis; biomarkers; ceramides; chromatography; ecosystems; genes; intestinal microorganisms; metabolic diseases; metabolism; metabolites; metabolomics; mice; microbiome; neomycin; neoplasms; phosphatidylcholines; phosphatidylethanolamines; proline; taurocholic acid; vancomycin; xenotransplantation
Abstract:
... Gut microbiota is associated with tumor progress and host metabolic disorder, but whether gut microbiota regulation can affect cancer growth through interfering host metabolism maintains unknown yet. Here, we used combined antibiotics (ABX) to build an extremely altered gut microbiota ecosystem and study its influence on the xenograft MC38 tumor as well as the associations of the effects with host ...
DOI:
10.1016/j.jchromb.2022.123136

36. HRPIF data mining based on data-dependent/independent acquisition for Rhei Radix et Rhizoma metabolite screening in rats

Author:
Jiayun Chen; Rong Ding; Ying Jin; Chunyan Zhu; Xiaojuan Jiang; Suping Wang; Zhen Li; Weihua Li; Caisheng Wu
Source:
Journal of chromatography 2022 v.1190 pp. 123095
ISSN:
1570-0232
Subject:
Oriental traditional medicine; anthraquinones; chromatography; data collection; metabolites; prototypes
Abstract:
... In traditional Chinese medicine (TCM), components with identical nuclei often share structural similarity, indicating the possibility of similar second-level mass spectrometry (MS/MS) fragments. High-resolution product-ion filter (HRPIF) technique can be utilized to identify metabolites, with similar fragments, in vivo. In principle, this technique applies to TCM; however, its application has been ...
DOI:
10.1016/j.jchromb.2021.123095

37. Improvement on recovery and reproducibility for quantifying urinary mono-hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs)

Author:
Khue Nguyen; Masha Pitiranggon; Hui-Chen Wu; Esther M. John; Regina M. Santella; Mary Beth Terry; Beizhan Yan
Source:
Journal of chromatography 2022 v.1192 pp. 123113
ISSN:
1570-0232
Subject:
acetonitrile; chemical species; environmental monitoring; epidemiology; hydroxylation; metabolites; methanol; polymers; quality control; sodium acetate; solid phase extraction; solvents; urine; variance; New York
Abstract:
... Efficient and reproducible measurements of multiple polycyclic aromatic hydrocarbon (PAH) metabolites in urinary samples are required to evaluate the complex health effects of PAH exposure. Here, we demonstrate a highly practical, automated off-line solid-phase extraction (SPE) of deconjugated hydroxylated PAHs followed by LC-MS/MS to simultaneously measure eight mono-hydroxylated PAH compounds: 1 ...
DOI:
10.1016/j.jchromb.2022.123113

38. Integrated approach toward absorption, distribution, metabolism, and excretion of Xiaoke pills in zebrafish based on UPLC-HRMS and DESI-MS techniques

Author:
Chunyan Zhu; Xiaojuan Jiang; Juanjuan Tian; Jiayun Chen; Chunyu Lin; Chong Wang; Cai Tie; Mingyu Li; Caisheng Wu
Source:
Journal of chromatography 2022 v.1200 pp. 123276
ISSN:
1570-0232
Subject:
Danio rerio; Oriental traditional medicine; absorption; daidzein; demethylation; desorption; electrospray ionization mass spectrometry; excretion; formononetin; glibenclamide; glycemic effect; glycosylation; hydroxylation; metabolites; research planning
Abstract:
... Traditional Chinese medicine (TCM) has been used in clinical settings for over 2000 years in China. The study of the absorption, distribution, metabolism, and excretion (ADME) of TCM in vivo could be beneficial for the discovery of the active components in TCM. However, the conventional strategies used for ADME research are based on rodent models and have the characteristics of lengthy experimenta ...
DOI:
10.1016/j.jchromb.2022.123276

39. Isotope labelled in suit derivatization-extraction integrated system for amine/phenol submetabolome analysis based on nanoconfinement effect: Application to lung cancer

Author:
Chunyu Yu; Qian Zhang; Yiwen Zhang; Liyuan Wang; Huarong Xu; Kaishun Bi; Donghao Li; Qing Li
Source:
Journal of chromatography 2022 v.1670 pp. 462954
ISSN:
0021-9673
Subject:
alkalinization; biomarkers; carbon nanofibers; chlorides; derivatization; detection limit; humans; isotopes; lung neoplasms; metabolites; metabolome; nanopores; phenol; therapeutics
Abstract:
... Amine/phenol submetabolome has shown critical role in clinical cancer screening and therapy. In suit derivatization has widely applied in the analysis of amine/phenol submetabolome by LC/MS for simplifying the pretreatment procedures, improving the separation and sensitivity. However, the complexity of biological matrix and trace amount of metabolites in plasma that lead to the limited detection c ...
DOI:
10.1016/j.chroma.2022.462954

40. Liquid Chromatography Method for Simultaneous Quantification of ATP and its Degradation Products Compatible with both UV-Vis and Mass Spectrometry

Author:
Andrew S. Law; Paul S. Hafen; Jeffrey J. Brault
Source:
Journal of chromatography 2022 pp. 123351
ISSN:
1570-0232
Subject:
ammonium acetate; chemical species; high performance liquid chromatography; hindlimbs; mass spectrometry; metabolites; mice; phosphates; ultra-performance liquid chromatography
Abstract:
... ATP and its degradation products are essential metabolic and signaling molecules. Traditionally, they have been quantified via high-performance liquid chromatography (HPLC) with UV-Vis detection while utilizing phosphate buffer mobile phase, but this approach is incompatible with modern mass detection. The goal of this study was to develop an ultra-performance liquid chromatography (UPLC) method f ...
DOI:
10.1016/j.jchromb.2022.123351

41. Liquid chromatography-mass spectrometry method for discovering the metabolic markers to reveal the potential therapeutic effects of naringin on osteoporosis

Author:
Yuanfeng Li; Jinyang Liu; Hongzheng Zhou; Juntong Liu; Xiaoying Xue; Longfei Wang; Shujun Ren
Source:
Journal of chromatography 2022 v.1194 pp. 123170
ISSN:
1570-0232
Subject:
amino acids; blood serum; fatty acid metabolism; liquid chromatography; mass spectrometry; metabolites; metabolomics; naringin; osteoporosis; pharmacology; prediction; pyruvic acid; therapeutics
Abstract:
... The pharmacological effects and therapeutic targets of naringin (NG) against osteoporosis (OP) is still unclear. Liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF/MS) based non-targeted metabonomics has been used to explore the differentiated metabolites and potential biological pathways of NG in the pathological process of OP. Using network pharmacology analysis, the key ...
DOI:
10.1016/j.jchromb.2022.123170

42. Liquid chromatography-tandem mass spectrometry based quantification of arginine metabolites including polyamines in different sample matrices

Author:
Mathias Langner; Ivona Mateska; Nicole Bechmann; Ben Wielockx; Triantafyllos Chavakis; Vasileia Ismini Alexaki; Mirko Peitzsch
Source:
Journal of chromatography 2022 v.1671 pp. 463021
ISSN:
0021-9673
Subject:
Parkinson disease; S-adenosylmethionine; T-lymphocytes; agmatine; arginine; biomarkers; cell culture; citrulline; essential amino acids; fatty liver; hydrophilic interaction chromatography; macrophage activation; metabolism; metabolites; mice; ornithine; putrescine; spermidine; spermine; tandem mass spectrometry
Abstract:
... The conditionally essential amino acid arginine and its metabolic products play an important role in different biological processes, such as metabolic regulation of the immune response, including macrophage activation and polarization and regulation of T cell function. Furthermore, the polyamine spermidine has a role in aging and age-related diseases. Additionally, altered polyamine metabolism may ...
DOI:
10.1016/j.chroma.2022.463021

43. Liquid-liquid extraction combined with online cleanup for the simultaneous determination of PAHs by GC–MS/MS and their hydroxylated metabolites by LC-MS/MS in human fingernails

Author:
Zihuan Zeng; Yuan Gao; Juntao Cui; Meiqing Lin; Jian Tang; Fei Wang; Yan Yang; Yingxin Yu; Shengtao Ma
Source:
Journal of chromatography 2022 v.1188 pp. 123057
ISSN:
1570-0232
Subject:
acetone; decontamination; environmental monitoring; ethyl acetate; fluorenes; gels; hexane; humans; hydroxylation; liquid chromatography; liquid-liquid extraction; metabolites; methylene chloride; naphthalene; permeability; phenanthrenes; sodium hydroxide; solid phase extraction; tandem mass spectrometry; China
Abstract:
... A method was developed for the simultaneous determination of polycyclic aromatic hydrocarbons (PAHs) and their hydroxylated metabolites (OH-PAHs) in human fingernails using liquid–liquid extraction and online purification. After surface decontamination by rinsing with acetone, the fingernails were digested with sodium hydroxide and subjected to liquid–liquid extraction with a mixture of n-hexane a ...
DOI:
10.1016/j.jchromb.2021.123057

44. Measurement of 5- fluorouracil, capecitabine and its metabolite concentrations in blood using volumetric absorptive microsampling technology and LC-MS/MS

Author:
Mirjana Radovanovic; Jennifer J. Schneider; Mohsen Shafiei; Jennifer H. Martin; Peter Galettis
Source:
Journal of chromatography 2022 v.1188 pp. 123075
ISSN:
1570-0232
Subject:
acetonitrile; ambient temperature; blood sampling; cancer therapy; chemical species; electrospray ionization mass spectrometry; fluorouracil; formic acid; health services; isotope labeling; liquid chromatography; metabolites; pharmacokinetics; toxicity
Abstract:
... 5-fluorouracil (5-FU) and its oral formulation, capecitabine, are widely used in treating a range of malignancies, either alone or in combination with other antineoplastic drugs. Body surface area-based dosing is used for these agents, despite this approach leading to substantial variability in drug exposure and often resulting in either toxicity or treatment failure. Tailoring therapeutic regimen ...
DOI:
10.1016/j.jchromb.2021.123075

45. Metabolomics analysis of urine from rats given long-term high-protein diet using ultra-high-performance liquid chromatography-mass spectrometry

Author:
Hua Ning; Dan Shi; Zhen Tian; Zhipeng Liu; Xinyue Wang; Xuemin Yan; Changhao Sun; Yucun Niu
Source:
Journal of chromatography 2022 v.1190 pp. 123082
ISSN:
1570-0232
Subject:
biomarkers; body weight; energy; food intake; high protein diet; liquid chromatography; males; mass spectrometry; metabolism; metabolites; metabolomics; models; rats; skeletal muscle; urinalysis; urine; weight loss
Abstract:
... Previous studies have indicated high-protein diet (HPD) promotes weight loss and improves metabolic parameters, but most of these studies have focused on the impact of short-term, long-term effects remain unclear. In this study, male Wistar rats were fed two diets for 88 weeks: normal control diet (NCD, 20.5% of energy as protein) or HPD (30.5% of energy as protein). At 88 weeks intervention, comp ...
DOI:
10.1016/j.jchromb.2021.123082

46. Minimum variance optimized Fisher ratio analysis of comprehensive two-dimensional gas chromatography / mass spectrometry data: Study of the pacu fish metabolome

Author:
Sonia Schöneich; Grant S. Ochoa; Celina M. Monzón; Robert E. Synovec
Source:
Journal of chromatography 2022 v.1667 pp. 462868
ISSN:
0021-9673
Subject:
Piaractus mesopotamicus; algorithms; automation; chemical species; comprehensive two-dimensional gas chromatography; derivatization; farmed fish; metabolites; metabolome; rice; variance; Argentina
Abstract:
... Integration of rice and fish farming, eg., pacu fish in Argentina, has raised concern that herbicides used for rice paddies may adversely affect the fish metabolome. To study this issue, tile-based Fisher ratio (F-ratio) analysis was applied to comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC × GC-TOFMS) data of pacu fish raised in an integrated rice-fish ...
DOI:
10.1016/j.chroma.2022.462868

47. Online biological sample preparation with restricted access hybrid carbon nanotubes for determination of anti-smoking drugs

Author:
Henrique Dipe de Faria; Alberto Thalison Silveira; Beatriz Carvalho do Prado; Julia Louise Moreira Nacif; Mariana Azevedo Rosa; Juliana da Rocha dos Santos; Paulo Caleb Júnior Lima Santos; Eduardo Costa Figueiredo; Isarita Martins
Source:
Journal of chromatography 2022 v.1669 pp. 462931
ISSN:
0021-9673
Subject:
adsorption; blood serum; bovine serum albumin; carbon; carbon nanotubes; chemical species; glycerol; humans; hydrophilicity; metabolites; nicotine; pH; spectrometers; temperature; therapeutics; thermal stability; ultra-performance liquid chromatography
Abstract:
... Untreated samples were injected directly into a column switching system, an online SPE technique, using an extraction column packed with restricted access hybrid carbon nanotubes (RAHCNTs), a novel type of restricted access material, in an ultra-high performance liquid chromatography, coupled to a mass spectrometer (UHPLC–MS/MS). The synthesis of used restricted access material was relatively simp ...
DOI:
10.1016/j.chroma.2022.462931

48. Optimization of gradient reversed phase high performance liquid chromatography analysis of acetaminophen oxidation metabolites using linear and non-linear retention model

Author:
Jana Vanova; David Malinak; Rudolf Andrys; Miroslav Kubat; Tomas Mikysek; Erika Rousarova; Kamil Musilek; Tomas Rousar; Petr Cesla
Source:
Journal of chromatography 2022 v.1669 pp. 462956
ISSN:
0021-9673
Subject:
acetaminophen; acetylcysteine; cysteine; detection limit; glutathione; humans; instrumentation; kidneys; liver; liver diseases; mass spectrometry; metabolism; metabolites; models; overdose; oxidation; toxicity
Abstract:
... Acetaminophen (paracetamol, APAP) is one of the most widely used drugs worldwide. Unfortunately, its overdose, which is caused by predominant oxidation of APAP, can lead to acute liver injury. In liver, oxidized APAP is conjugated with glutathione, leading to APAP-glutathione conjugate, which is metabolized to APAP-cysteine and APAP-N-acetylcysteine conjugates. Thus, all of those compounds could b ...
DOI:
10.1016/j.chroma.2022.462956

49. Potential efficacious materials investigation of Yi-Yi Mixture based on Metabolome-oriented network pharmacology strategy

Author:
Guan-wen Gong; Wei-hong Tang; Zhuo Zhou; Yan-wen Jiang; Cui-zhong Wang; Hui Cheng; Yuan Cao; Zhi-wei Jiang
Source:
Journal of chromatography 2022 v.1197 pp. 123199
ISSN:
1570-0232
Subject:
Oriental traditional medicine; emodin; lignans; mass spectrometry; metabolites; metabolome; pancreatitis; pharmacodynamics; quality control; rats; resveratrol; ultra-performance liquid chromatography; China
Abstract:
... Yi-Yi Mixture, an efficient Chinese medicine preparation composed of four herbal medicines, has been used in clinical practice in China for the treatment of acute pancreatitis over twenty years. However, its functional materials against acute pancreatitis remains unclear, which is a huge obstacle for quality control. In this study, a metabolome-oriented network pharmacology strategy was proposed t ...
DOI:
10.1016/j.jchromb.2022.123199

50. Qualitative and semi-quantitative screening of selected psychoactive substances in blood: Usefulness of liquid chromatography – triple quadrupole and quadrupole time-of-flight mass spectrometry in routine toxicological analyses

Author:
Maarten Degreef; Eleanor M. Berry; Adrian Covaci; Kristof E.K. Maudens; Alexander L.N. van Nuijs
Source:
Journal of chromatography 2022 pp. 123279
ISSN:
1570-0232
Subject:
antipsychotics; benzodiazepines; blood; chemical species; data collection; databases; human resources; liquid chromatography; liquid-liquid extraction; mass spectrometry; metabolites; therapeutics; toxicity testing
Abstract:
... Routine toxicological analysis requires broad screening for a large number of therapeutically prescribed and other compounds, and/or their metabolites. This article specifically focuses on three classes of psychoactive substances: antidepressants (ADs), antipsychotics (APs) and benzodiazepines and Z-drugs (BZDs). Two screening methods were compared for their ease-of-use in a routine setting, based ...
DOI:
10.1016/j.jchromb.2022.123279