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- Boonchit, S.; Xuan, X.; Yokoyama, N.; Goff, W.L.; Wagner, G.; Igarashi, I.
- Journal of clinical microbiology 2002 v.40 no.10 pp. 3771
- babesiosis; cattle diseases; disease diagnosis; disease detection; Babesia bovis; enzyme-linked immunosorbent assay; recombinant fusion proteins; antigen-antibody reactions
- ... The gene encoding Babesia bovis rhoptry-associated protein 1 (RAP-1) was used to develop an enzyme-linked immunosorbent assay (ELISA) to measure specific antibodies against B. bovis. The B. bovis RAP-1 gene was subcloned into a baculovirus transfer vector, and the RAP-1 protein was expressed in insect cells infected with a recombinant baculovirus. The recombinant B. bovis RAP-1 of 65 kDa was detec ...
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