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- Chang, H.Y.; Xie, R.X.; Zhang, L.; Fu, L.Z.; Zhang, C.T.; Chen, H.H.; Wang, Z.Q.; Zhang, Y.; Quan, F.S.
- Journal of dairy science 2019 v.102 no.5 pp. 4662-4673
- physiological state, etc ; Holstein; apoptosis; blastocyst; cell cycle; cell proliferation; cows; early development; embryogenesis; fibroblasts; gene overexpression; genes; messenger RNA; microRNA; protein content; somatic cells; Show all 16 Subjects
- ... Accumulating studies have suggested that microRNA play a part in regulating multiple cellular processes, such as cell proliferation, apoptosis, the cell cycle, and embryo development. This study explored the effects of miR-101-2 on donor cell physiological status and the development of Holstein cow somatic cell nuclear transfer (SCNT) embryos in vitro. Holstein cow bovine fetal fibroblasts (BFF) o ...
- Khatib, H.; Gross, N.
- Journal of dairy science 2019 v.102 no.4 pp. 3744-3753
- physiological state, etc ; RNA interference; biomarkers; biopsy; cattle; diet; embryogenesis; embryonic mortality; environmental factors; gene editing; genes; genomics; humans; microRNA; pregnancy outcome; risk; transcriptome; transcriptomics; uterus; Show all 19 Subjects
- ... The majority of embryonic loss in cattle occurs within the first 3 to 4 wk of pregnancy, and there are currently no accurate predictors of pregnancy outcome. Existing embryo quality assessment methods include morphological evaluation and embryo biopsy. These methods are not accurate and carry some health risks to the developing embryo, respectively. Therefore, there is need to identify noninvasive ...