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- Pupillo, P., et al. Show all 3 Authors
- Journal of experimental botany 1998 v.49 no.325 pp. 1307-1315
- Spinacia oleracea; isozymes; chloroplasts; glyceraldehyde-3-phosphate dehydrogenase; plant extracts; molecular weight; enzyme activity; chromatography; pH; enzyme inhibitors; catalysts; fractionation; NADP (coenzyme); NAD (coenzyme)
- ... Isoforms of NAD(P)-dependent glyceraldehyde-3- phosphate dehydrogenases (EC 18.104.22.168) have been separated from spinach chloroplast extracts by FPLC-anion exchange chromatography in phosphate buffer and purified to homogeneity. Peak I from Q-Sepharose corresponds to a tetramer of A-subunits of 36 kDa showing a constant ratio of NADPH- to NADH-activity of 2 (insensitive to substrate-modulators), and ...