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- Author:
- Colussi, S; Prearo, M; Bertuzzi, S A; Scanzio, T; Peletto, S; Favaro, L; Modesto, P; Maniaci, M G; Ru, G; Desiato, R; Acutis, P L
- Source:
- Journal of fish diseases 2015 v.38 no.1 pp. 27-35
- ISSN:
- 0140-7775
- Subject:
- Lactococcus garvieae; Oncorhynchus mykiss; T-lymphocytes; antibiotic residues; antibiotics; bacterial infections; breeding; codons; exons; financial economics; fish development; freshwater; glycoproteins; haplotypes; immune response; industry; loci; major histocompatibility complex; marine fish; peptides; profitability; single nucleotide polymorphism; vaccines; water pollution
- Abstract:
- ... Major histocompatibility complex (MHC) loci encode glycoproteins that bind to foreign peptides and initiate immune responses through their interaction with T cells. MHC class II molecules are heterodimers consisting of α and β chains encoded by extremely variable genes; variation in exon 2 is responsible for the majority of observed polymorphisms, mostly concentrated in the codons specifying the p ...
- DOI:
- 10.1111/jfd.12193
-
http://dx.doi.org/10.1111/jfd.12193
- Author:
- Ture, M; Altinok, I; Capkin, E
- Source:
- Journal of fish diseases 2015 v.38 no.1 pp. 37-47
- ISSN:
- 0140-7775
- Subject:
- DNA; Lactococcus garvieae; biotypes; consensus sequence; polymerase chain reaction; pulsed-field gel electrophoresis; repetitive sequences; restriction endonucleases; France; Italy; Spain
- Abstract:
- ... Biochemical test, pulsed‐field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus sequence PCR (ERIC‐PCR) were used to compare 42 strains of Lactococcus garvieae isolated from different regions of Turkey, Italy, France and Spain. Twenty biotypes of L. garvieae were formed based on 54 biochemical tests. ERIC‐PCR of genomic DNA from different L. garvieae strains resulted ...
- DOI:
- 10.1111/jfd.12192
-
http://dx.doi.org/10.1111/jfd.12192
- Author:
- Sharon, G; Pimenta‐Leibowitz, M; Vilchis, M C L; Isakov, N; Zilberg, D
- Source:
- Journal of fish diseases 2015 v.38 no.1 pp. 67-74
- ISSN:
- 0140-7775
- Subject:
- Poecilia reticulata; Tetrahymena; adaptive immunity; cold; cold stress; commercial farms; fish; histopathology; integument; intraperitoneal injection; lethal dose 50; lysozyme; mortality; netting; parasites
- Abstract:
- ... Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infect ...
- DOI:
- 10.1111/jfd.12204
-
http://dx.doi.org/10.1111/jfd.12204
- Author:
- Henriksen, M M M; Kania, P W; Buchmann, K; Dalsgaard, I
- Source:
- Journal of fish diseases 2015 v.38 no.1 pp. 55-66
- ISSN:
- 0140-7775
- Subject:
- Flavobacterium psychrophilum; Oncorhynchus mykiss; antibodies; antibody formation; blood; fish; gene expression; genes; hydrogen peroxide; kidneys; microbial load; pathogens; pretreatment; ribosomal RNA
- Abstract:
- ... The immune response in rainbow trout fry against Flavobacterium psychrophilum was elucidated using an immersion‐based challenge with or without prior exposure to hydrogen peroxide (H₂O₂). Samples were taken from the head kidney 4, 48, 125 and 192 h after immersion, and the regulation of several genes was examined. Bacterial load was assessed based on the presence of 16S rRNA and correlated with ge ...
- DOI:
- 10.1111/jfd.12201
-
http://dx.doi.org/10.1111/jfd.12201
- Author:
- Low, C‐F; Nor Shamsudin, M; Abdullah, M; Chee, H‐Y; Aliyu‐Paiko, M
- Source:
- Journal of fish diseases 2015 v.38 no.1 pp. 17-25
- ISSN:
- 0140-7775
- Subject:
- Epinephelus fuscoguttatus; Vibrio parahaemolyticus; amino acid composition; apolipoprotein E; blood serum; disease resistance; fingerlings; gene expression regulation; grouper; immune response; immunoglobulin E; immunoglobulin light chains; proteome; two-dimensional gel electrophoresis
- Abstract:
- ... The mechanisms through which brown‐marbled grouper accomplishes resistance to infection, particularly against Vibrios, are not yet fully understood. In this study, brown‐marbled grouper fingerlings were experimentally infected with Vibrio parahaemolyticus, to identify disease resistance grouper, and the serum proteome profiles were compared between resistant and susceptible candidates, via two‐dim ...
- DOI:
- 10.1111/jfd.12195
-
http://dx.doi.org/10.1111/jfd.12195
- Author:
- Keawcharoen, J; Techangamsuwan, S; Ponpornpisit, A; Lombardini, E D; Patchimasiri, T; Pirarat, N
- Source:
- Journal of fish diseases 2015 v.38 no.1 pp. 49-54
- ISSN:
- 0140-7775
- Subject:
- Oreochromis niloticus; Redspotted grouper nervous necrosis virus; coat proteins; disease outbreaks; farmed fish; genes; immunohistochemistry; larvae; necrosis; nucleotide sequences; phylogeny; viruses; Thailand
- Abstract:
- ... Betanodavirus infection was diagnosed in larvae of farm‐raised tilapia Oreochromis niloticus (L.), in central Thailand. Extensive vacuolar degeneration and neuronal necrosis were observed in histological sections with positive immunohistochemical staining for betanodavirus. Molecular phylogenetic analysis was performed based on the nucleotide sequences (1333 bases) of the capsid protein gene. The ...
- DOI:
- 10.1111/jfd.12200
-
http://dx.doi.org/10.1111/jfd.12200
- Author:
- McBeath, A; Aamelfot, M; Christiansen, D H; Matejusova, I; Markussen, T; Kaldhusdal, M; Dale, O B; Weli, S C; Falk, K
- Source:
- Journal of fish diseases 2015 v.38 no.1 pp. 3-15
- ISSN:
- 0140-7775
- Subject:
- Infectious salmon anemia virus; Salmo salar; blood; disease course; fish; gills; immunohistochemistry; mortality; nucleoproteins; pathogenesis; quantitative polymerase chain reaction; viral load; virulence; viruses
- Abstract:
- ... The salmonid orthomyxovirus infectious salmon anaemia virus (ISAV) causes disease of varying severity in farmed Atlantic salmon, Salmo salar L. Field observations suggest that host factors, the environment and differences between ISAV strains attribute to the large variation in disease progression. Variation in host mortality and dissemination of ISAV isolates with high and low virulence (based on ...
- DOI:
- 10.1111/jfd.12253
-
http://dx.doi.org/10.1111/jfd.12253
- Author:
- Fraser, T W K; Hansen, T; Fleming, M S; Fjelldal, P G
- Source:
- Journal of fish diseases 2015 v.38 no.1 pp. 75-89
- ISSN:
- 0140-7775
- Subject:
- Salmo salar; diploidy; egg incubation; eggs; farmed fish; parr; rearing; risk factors; skeletal development; temperature; triploidy; vertebrae
- Abstract:
- ... Suboptimal egg incubation temperature is a risk factor for the development of skeletal deformities in teleosts. Triplicate diploid and triploid Atlantic salmon, Salmo salar L., egg batches were incubated at 6, 8 and 10 °C up until first feeding, whereupon fish were reared on a natural temperature before examination for externally visible skeletal deformities (jaw and spine) and radiographed for ve ...
- DOI:
- 10.1111/jfd.12206
-
http://dx.doi.org/10.1111/jfd.12206