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- Senphan, Theeraphol; Benjakul, Soottawat; Kishimura, Hideki
- Journal of food biochemistry 2015 v.39 no.4 pp. 388-397
- trypsin inhibitors, etc ; Litopenaeus vannamei; agarose; ammonium sulfate; chymotrypsin; enzyme kinetics; food processing; heat stability; heat treatment; hepatopancreas; hydrolysis; income; molecular weight; pH; polyacrylamide gel electrophoresis; shrimp; sodium; soybeans; temperature; trypsin; Show all 20 Subjects
- ... Trypsin from hepatopancreas of Pacific white shrimp (Litopenaeus vannamei) was purified to homogeneity using ammonium sulfate precipitation and a series of chromatographies including diethylaminoethyl sepharose and soybean trypsin inhibitor sepharose 4B columns. Trypsin was purified to 50.4‐fold with a yield of 13.7%. Based on native‐polyacrylamide gel electrophoresis (PAGE), the purified trypsin ...