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- Wesley, I.V.; Harmon, K.M.; Dickson, J.S.; Schwartz, R.
- Journal of food protection 2002 v.65 no.5 pp. 780
- turkeys; meat carcasses; turkey meat; Listeria monocytogenes; Listeria; food contamination; microbial contamination; detection; isolation; genetic variation; polymerase chain reaction; assays; rapid methods; ribosomal RNA
- ... A multiplex polymerase chain reaction was developed to simultaneously identify Listeria monocytogenes and species of the genus Listeria. Two sets of primers were used, with the first amplifying a 938-bp region of the 16S rRNA gene that is highly conserved in all Listeria species and the second amplifying a 174-bp region of the listeriolysin (hlyA) gene of L. monocytogenes. Thus, isolates of Lister ...