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- Li, Na; Wang, Li; Wang, Hui; Ma, Minyue; Wang, Xiaohong; Li, Yi; Zhang, Wenke; Zhang, Jianguang; Cram, David S.; Yao, Yuanqing
- Journal of genetics and genomics 2015 v.42 no.4 pp. 151-159
- DNA; biopsy; chromosome aberrations; chromosomes; genome; high-throughput nucleotide sequencing; patients; polymerase chain reaction; polyploidy
- ... Reliable and accurate pre-implantation genetic diagnosis (PGD) of patient's embryos by next-generation sequencing (NGS) is dependent on efficient whole genome amplification (WGA) of a representative biopsy sample. However, the performance of the current state of the art WGA methods has not been evaluated for sequencing. Using low template DNA (15 pg) and single cells, we showed that the two PCR-ba ...