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You searched for: Subject "culture media" Remove constraint Subject: "culture media" Journal Journal of horticultural science & biotechnology Remove constraint Journal: Journal of horticultural science & biotechnology Publication Year 2017 Remove constraint Publication Year: 2017 Subject callus Remove constraint Subject: callus
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1. Somatic embryogenesis of Limonium sinense: a study on the regeneration efficacy via direct and indirect approach followed by Agrobacterium-mediated genetic transformation

Author:
Dam, Anandamoy; Bhattacharya, Chayanika; Bandyopadhyay, Tapas K.
Source:
Journal of horticultural science & biotechnology 2017 v.92 no.4 pp. 411-423
ISSN:
1462-0316
Subject:
culture media,   etc   ; 2,4-D; Agrobacterium radiobacter; Limonium; biotechnology; callus; explants; genes; genetic transformation; horticulture; kanamycin; kanamycin kinase; leaves; naphthaleneacetic acid; plantlets; polymerase chain reaction; somatic embryogenesis; somatic embryos; transgenic plants;     Show all 19 Subjects
Abstract:
... An efficient indirect somatic embryogenesis and Agrobacterium-mediated transformation protocol for Limonium sinense has been established, wherein neomycin phosphotransferase II (npt II) and ß-glucuronidase (GUS) genes were used as selectable and screenable markers, respectively. The efficiency of plantlet regeneration from transformed tissue was compared between direct embryogenesis from leaf ...
DOI:
10.1080/14620316.2017.1286952