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- Liang, Keming; Shen, Claire R.
- Journal of industrial microbiology & biotechnology 2017 v.44 no.12 pp. 1605-1612
- Clostridium beijerinckii; Escherichia coli; Klebsiella pneumoniae; NAD (coenzyme); NADP (coenzyme); acetoin; biosynthesis; engineering; fermentation; phosphates; redox reactions
- ... Enzymatic reduction of acetoin into 2,3-butanediol (2,3-BD) typically requires the reduced nicotinamide adenine dinucleotide (NADH) or its phosphate form (NADPH) as electron donor. Efficiency of 2,3-BD biosynthesis, therefore, is heavily influenced by the enzyme specificity and the cofactor availability which varies dynamically. This work describes the engineering of cofactor flexibility for 2,3-B ...