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- Jackson, N.; Wu, T.Z.; Adams-Sapper, S.; Satoorian, T.; Geisberg, M.; Murthy, N.; Lee, L.; Riley, L.W.
- Journal of microbiological methods 2019 v.158 pp. 52-58
- Escherichia coli; Gram-negative bacteria; Pseudomonas aeruginosa; antibodies; diagnostic techniques; enzyme-linked immunosorbent assay; glucose; hospitals; pathogens
- ... Gram-negative bacteria (GNB) are important causes of community (CA) and hospital (HA)- associated infections. Here we describe the development of an indirect ELISA (I-ELISA), which can be used to detect and differentiate the Enterobacteriaceae Escherichia coli, and glucose non-fermenter Pseudomonas aeruginosa from other GNB species. The I-ELISA utilizes six antibodies for bacterial speciation, whi ...
- Bubnov, Dmitrii M.; Yuzbashev, Tigran V.; Vybornaya, Tatiana V.; Netrusov, Alexander I.; Sineoky, Sergey P.
- Journal of microbiological methods 2019 v.158 pp. 86-92
- DNA; Escherichia coli; antibiotic resistance; antibiotic resistance genes; chromosomes; electroporation; genetic engineering; mutation
- ... The introduction of chromosomal mutations into the E. coli genome using λRed-mediated recombineering includes two consecutive steps—the insertion of an antibiotic resistance gene and the subsequent excision of the marker. The second step usually requires a counterselection method, because the efficiency of recombination is not high enough to find recombinants among non-recombinant cells. Most coun ...