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You searched for: Journal name Journal of microbiological methods Remove constraint Journal name: Journal of microbiological methods Publication year rev 7981-2019 Remove constraint Publication year rev: 7981-2019 Source 2019 v.167 Remove constraint Source: 2019 v.167
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1. Baby-SPIME: A dynamic in vitro piglet model mimicking gut microbiota during the weaning process

Author:
Sandrine Dufourny; Nadia Everaert; Sarah Lebrun; Caroline Douny; Marie-Louise Scippo; Bing Li; Bernard Taminiau; Massimo Marzorati; José Wavreille; Eric Froidmont; Pierre Rondia; Véronique Delcenserie
Source:
Journal of microbiological methods 2019 v.167 pp. 105735
ISSN:
0167-7012
Subject:
Bacteroides; Bifidobacterium; Fusobacterium; Lactobacillus; Megasphaera; Mitsuokella; Prevotella; acetates; animal models; bioreactors; butyric acid; colon; delta-Proteobacteria; fermentation; gamma-Proteobacteria; humans; ileum; in vitro digestion; intestinal microorganisms; metabolites; microbial communities; piglets; propionic acid; weaning
Abstract:
... The study aimed to adapt the SHIME® model, developed to simulate human digestion and fermentation, to a baby-SPIME (baby Simulator of Pig Intestinal Microbial Ecosystem). What constitutes a unique feature of this model is its twofold objective of introducing an ileal microbial community and mimicking a dietary weaning transition. This model should then be ideally suited to test the dietary weaning ...
DOI:
10.1016/j.mimet.2019.105735

2. Contrasting common measures of arbuscular mycorrhizal fungal root colonization

Author:
Vasilis Kokkoris; Antreas Pogiatzis; Miranda M. Hart
Source:
Journal of microbiological methods 2019 v.167 pp. 105727
ISSN:
0167-7012
Subject:
Linum usitatissimum; image analysis; mycorrhizal fungi; root systems; vesicular arbuscular mycorrhizae
Abstract:
... Estimating the abundance of arbuscular mycorrhizal fungi relies entirely on indirect methods, meaning all measures are associated with some variability. The most common methods use microscopic estimates of the relative proportion of root length colonized by fungal structures. These methods typically examine root subsamples. While such methods are inexpensive and relatively simple, significant vari ...
DOI:
10.1016/j.mimet.2019.105727

3. Development of a new serological assay for the diagnosis of Clostridium difficile infections with prognostic value

Author:
Felix von Bechtolsheim; Adorjan Varga; Laszlo Szereday; Beata Polgar; Timea Balassa; Bela Kocsis; Zoltan Peterfi; Eva Miko
Source:
Journal of microbiological methods 2019 v.167 pp. 105777
ISSN:
0167-7012
Subject:
Clostridium difficile; antibodies; blood serum; clinical trials; enzyme-linked immunosorbent assay; humans; humoral immunity; immune response; nutritional status; patients; prognosis; protective effect; risk; virulence
Abstract:
... The most common hospital-acquired enteral infection is caused by Clostridium difficile. Unfortunately, Clostridium difficile infections (CDI) are of high risk to recur and little is known about how to predict recurrences. Previous findings have shown that high risk for recurrence correlates with low levels of C. difficile toxin-A and -B specific antibodies suggesting the protective role of humoral ...
DOI:
10.1016/j.mimet.2019.105777

4. New avenues of controlling microbial infections through anti-microbial and anti-biofilm potentials of green mono-and multi-metallic nanoparticles: A review

Author:
Palashpriya Das; Vijayshree S. Karankar
Source:
Journal of microbiological methods 2019 v.167 pp. 105766
ISSN:
0167-7012
Subject:
antibiotics; antimicrobial properties; biofilm; biological resources; cost effectiveness; energy efficiency; microorganisms; multiple drug resistance; nanoparticles; plant products; resistance mechanisms; sustainable technology; toxicity; wastes
Abstract:
... Nanoparticles synthesized through the green route deserve special mention because this green technology is not only energy-efficient and cost-effective but also amenable to the environment. Various biological resources have been used for the generation of these ‘green nanoparticles’. Biological wastes have also been focused in this direction thereby promoting the value of waste. Reports indicate t ...
DOI:
10.1016/j.mimet.2019.105766

5. Quantitative assessment of bacterial growth phase utilizing flow cytometry

Author:
K.M. Khomtchouk; M. Weglarz; L.A. Bekale; I. Koliesnik; P.L. Bollyky; P.L. Santa Maria
Source:
Journal of microbiological methods 2019 v.167 pp. 105760
ISSN:
0167-7012
Subject:
Pseudomonas aeruginosa; animal pathogens; bacterial growth; biofilm; chronic diseases; developmental stages; dyes; flow cytometry; phenotype; plankton; polystyrenes; public health
Abstract:
... Flow cytometry is currently underutilized for bacterial phenotyping and standard microbiological techniques do not provide phenotypic information about the state of the bacterial disease. Pseudomonas aeruginosa is a human pathogen of increased importance in public health due to both the ability to cause chronic diseases and the prevalence of functionally different subsets that can be difficult to ...
DOI:
10.1016/j.mimet.2019.105760
PubMed:
31678132
PubMed Central:
PMC6957528

6. Viability assessment of Bifidobacterium longum ATCC 15707 on non-dairy foods using quantitative fluorescence microscopy

Author:
Min Min; Susan L. Mason; Grant N. Bennett; Malik A. Hussain; Craig R. Bunt
Source:
Journal of microbiological methods 2019 v.167 pp. 105778
ISSN:
0167-7012
Subject:
Bifidobacterium longum; agar; bacteria; beneficial microorganisms; cell membranes; density gradient centrifugation; drying; filtration; fluorescence; fluorescence microscopy; food matrix; ingredients; oats; peanuts; product development; quality control; rapid methods; rehydration; staining; temperature; viability
Abstract:
... This study demonstrates an effective technique for separating and purifying viable bacteria from samples that interfere with viability staining. The viability of Bifidobacterium longum ATCC 15707 was assessed using Percoll Buoyant Density Gradient Centrifugation (PBDC) to separate bacteria from complex non-dairy food matrices and Quantitative Fluorescence Microscopy (QFM) to determine individual c ...
DOI:
10.1016/j.mimet.2019.105778

7. A method to assess bioavailability of antibiotics in anthropogenic polluted ecosystems by using a bacterial fitness test

Author:
Massimiliano Marvasi; Alessandro Canali; Brunella Perito; Ajit J. Shah; Vlad Serafim
Source:
Journal of microbiological methods 2019 v.167 pp. 105724
ISSN:
0167-7012
Subject:
antibacterial properties; antibiotic residues; antibiotics; bacteria; bioassays; bioavailability; ecosystems; polluted soils; uncertainty
Abstract:
... Antibiotics released in the environment exert a selective pressure on the resident microbiota. It is well accepted that the mere measurement of antibiotics does not reflect the actual bioavailability. In fact, antibiotics can be adsorbed or complexed to particles and/or chemicals in water and soil. Bioavailable concentrations of antibiotics in soil and water are subjected to great uncertainty, the ...
DOI:
10.1016/j.mimet.2019.105724

8. Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays

Author:
Mandy Mun Yee Kwong; Jee Whu Lee; Mohammed Razip Samian; Nobumoto Watanabe; Hiroyuki Osada; Eugene Boon Beng Ong
Source:
Journal of microbiological methods 2019 v.167 pp. 105743
ISSN:
0167-7012
Subject:
Saccharomyces cerevisiae; aeration; longevity; low calorie diet; yeasts
Abstract:
... This study compared the chronological life span and survival of Saccharomyces cerevisiae aged in a microplate or bottle, under different aeration and calorie restriction conditions. Our data shows that limited aeration in the microplate-aged culture contributed to slower outgrowth but extended yeast CLS compared to the bottle-aged culture. ...
DOI:
10.1016/j.mimet.2019.105743

9. Detection of extended-spectrum β-lactamases producing Enterobacteriaceae using a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based MBT STAR-BL software module with β-lactamase inhibition assay depends on the bacterial strains

Author:
Yusuke Ota; Kazuki Furuhashi; Yuki Nagao; Takamasa Nanba; Katsumasa Yamanaka; Jinko Ishikawa; Osanori Nagura; Moriya Iwaizumi; Etsuko Hamada; Masato Maekawa
Source:
Journal of microbiological methods 2019 v.167 pp. 105734
ISSN:
0167-7012
Subject:
Enterobacteriaceae; bacteria; beta-lactamase; cefotaxime; clavulanic acid; computer software; desorption; disease control; enzyme activity; enzyme inhibition; enzyme inhibitors; hydrolysis; matrix-assisted laser desorption-ionization mass spectrometry; sulbactam
Abstract:
... Rapid and sensitive detection of extended-spectrum β-lactamases (ESBLs) is essential for infection control and antimicrobial treatment. Recently, a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based MBT STAR-BL software module has been used for detecting β-lactamase activity; however, this system cannot differentiate ESBL producing bacteria from other ...
DOI:
10.1016/j.mimet.2019.105734

10. Detection of plasmid-mediated colistin resistance by colistin pre-diffusion and inhibition with EDTA test (CPD-E) in Enterobactereaceae

Author:
Katherine Yauri Condor; Edgar Gonzales Escalante; José Di Conza; Gabriel Gutkind
Source:
Journal of microbiological methods 2019 v.167 pp. 105759
ISSN:
0167-7012
Subject:
Enterobacteriaceae; antibiotic resistance; colistin; microorganisms; phenotype
Abstract:
... A phenotypic assay based on colistin pre-diffusion and differential inhibition of Mobile Colistin Resistance (MCR) protein activity by EDTA showed that, of the 92 strains tested, all MCR producers (49) exhibited an increase ≥5 mm in the inhibition zone around the area of pre-diffusion in the presence of EDTA, in comparison with colistin alone. Results suggest that CPD-E may differentiate MCR-produ ...
DOI:
10.1016/j.mimet.2019.105759

11. Evaluation of TD test for analysis of persistence or tolerance in clinical isolates of Staphylococcus aureus

Author:
Hana Kotková; Marie Cabrnochová; Irena Lichá; Jan Tkadlec; Libor Fila; Jana Bartošová; Oto Melter
Source:
Journal of microbiological methods 2019 v.167 pp. 105705
ISSN:
0167-7012
Subject:
Staphylococcus aureus; bacteria; ciprofloxacin; cystic fibrosis; disk diffusion antimicrobial test; oxacillin; patients; vancomycin
Abstract:
... Besides natural and acquired mechanisms of resistance, bacteria can cope with presence of antibiotics by using complex mechanisms such as persistence or tolerance.The main purpose of this study was to evaluate the suitability of newly developed Tolerance Disk Test (TDtest) (Gefen et al., 2017) to detect persistent or tolerant bacterial cells in clinical isolates of Staphylococcus aureus. The princ ...
DOI:
10.1016/j.mimet.2019.105705

12. Examination of total cell count in building materials by acridine orange direct count (AODC)

Author:
Judith Meider
Source:
Journal of microbiological methods 2019 v.167 pp. 105725
ISSN:
0167-7012
Subject:
acridine orange; cell viability; construction materials; microbial contamination
Abstract:
... The Acridine orange direct count (AODC) enables the determination of total cell count (TCC) in building materials. The AODC technique is considered as an analytic standard in many different areas but is not common in the indoor environment. In this paper AODC was tested with various building materials to investigate TCC. Colony forming units (CFU) only detect viable cells in contrast to the TCC as ...
DOI:
10.1016/j.mimet.2019.105725

13. Synthesis and characterization of nanoparticles and composites as bactericides

Author:
Laleh R. Kalankesh; Susana Rodríguez-Couto; Mohammad Ali Zazouli; Yousef Dadban Shahamat; Ramazan Ali Dianati; Mahdieh Arghiani
Source:
Journal of microbiological methods 2019 v.167 pp. 105736
ISSN:
0167-7012
Subject:
Enterobacter aerogenes; Escherichia coli; antibacterial properties; antibiotics; chlorine; disinfectants; disk diffusion antimicrobial test; microbiological quality; microorganisms; nanocomposites; nanoparticles; silica; sol-gel processing; titanium dioxide; zeolites
Abstract:
... In the present study, SiO₂-TiO₂ hybrid nanomaterial and zeolite-TiO₂ (Z-TiO₂) composites were synthesized by the sol-gel method. Then, the antibacterial activity of the above-mentioned synthesized materials, SiO₂ and zeolite (Z) was investigated by the disk diffusion method using Echerichia coli and Enterobacter aerogenes as test microorganisms. All the materials showed antibacterial activity agai ...
DOI:
10.1016/j.mimet.2019.105736

14. An alternative disk diffusion test in broth and macrodilution method for colistin susceptibility in Enterobacteriales

Author:
López-Jácome Luis Esaú; Rengel-Garcia Christian Rodolfo; Hernández-Durán Melissa; Colín-Castro Claudia Adriana; García-Contreras Rodolfo; Franco-Cendejas Rafael
Source:
Journal of microbiological methods 2019 v.167 pp. 105765
ISSN:
0167-7012
Subject:
Enterobacteriales; antibiotic resistance; colistin; disk diffusion antimicrobial test; minimum inhibitory concentration; multiple drug resistance; polymyxin B
Abstract:
... Colistin and polymyxin B are old drugs that have been reintroduced to treat Gram-negative infections lacking other treatment options; however, colistin resistance have been reported. To know the correct susceptibility pattern is mandatory for multidrug resistant bacteria. Broth microdilution method is the gold standard to evaluate colistin and polymyxin B susceptibility; nevertheless, it is time c ...
DOI:
10.1016/j.mimet.2019.105765

15. “MINES” method for genomic DNA extraction from deep biosphere biofilms

Author:
Tanvi Govil; Wageesha Sharma; Neeraj K. Chauhan; Sudhir Kumar; David R. Salem; Rajesh K. Sani
Source:
Journal of microbiological methods 2019 v.167 pp. 105730
ISSN:
0167-7012
Subject:
DNA; Escherichia coli; Geobacillus; biofilm; biosphere; composts; enzymes; exopolysaccharides; genes; landfills; metagenomics; microbial communities; microorganisms; molecular weight; ribosomal RNA
Abstract:
... Successful and efficient extraction of high quality, high molecular weight genomic DNA from the environmental samples is an essential primary step to understand the genetic, metabolic and evolutionary characteristics of the microbial communities. Deep mine biofilm samples that contain high amounts of mucoid exopolysaccharide often pose difficulties to obtaining refined community DNA. To circumvent ...
DOI:
10.1016/j.mimet.2019.105730