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- Shimazaki, Youji; Takahashi, Ayumi
- Journal of microbiological methods 2018 v.154 pp. 19-24
- Bacillus subtilis; Escherichia coli; Gram-negative bacteria; antibacterial properties; cathodes; digital images; egg albumen; isoelectric focusing; lysozyme; ovalbumin; phosphoric acid; sodium hydroxide; two-dimensional gel electrophoresis
- ... The purpose of this study was to establish a method for determining the bacteriolytic activity after separation of lysozyme-binding proteins from egg white. Lysozyme-binding proteins such as ovotransferrin and ovalbumin were separated by non-denaturing two-dimensional electrophoresis (2DE) and transferred to a membrane. The lysozyme activity of the separated and immobilized egg white proteins was ...