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- Hu, Jinqiang; Huang, Runna; Wang, Yi; Wei, Xiangke; Wang, Zhangcun; Geng, Yao; Jing, Jianzhou; Gao, Hui; Sun, Xincheng; Dong, Caiwen; Jiang, Chunpeng
- Journal of microbiological methods 2018 v.154 pp. 127-133
- Escherichia coli O157; Salmonella; antibodies; bacteria; beef; biotin; cabbage; chickens; color; detection limit; digoxin; enzyme-linked immunosorbent assay; food sanitation; foodborne illness; genes; juices; microbial detection; milk; monitoring; nucleic acid hybridization; polymerase chain reaction; pork; shrimp; streptomycin
- ... In the current study, a duplex PCR-ELISA method was developed targeting the specific genes, invA of Salmonella spp. and rfbE of Escherichia coli O157: H7, to detect one or both bacteria in food. In brief, PCR product amplified by PCR primer labeled with digoxin at the 5′-end and a probe labeled with biotin at the 3′-end can form dimer by nucleic acid hybridization which can be captured by binding ...