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- Pham, Nam; Dao, Nelson; Saviola, Beatrice
- Journal of microbiological methods 2018 v.154 pp. 112-117
- Mycobacterium smegmatis; Mycobacterium tuberculosis; carotenoids; cell growth; gene overexpression; genes; macrophages; pH; phagosomes; plasmids; protein synthesis; proteins
- ... As inducible protein expression plasmids available for mycobacterial species are limited, here we demonstrate the utility of an acid-inducible promoter driving gene and subsequent protein expression of a difficult to express protein. We wanted to assess the use of an acid inducible promoter expression system to produce proteins in a mycobacterial system, specifically proteins that when overexpress ...