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- Author:
- Lekang, Katrine; Hadziavdic, Kenan; Sandnes Skaar, Katrine; Jonassen, Inge; Thompson, Eric M.; Troedsson, Christofer
- Source:
- Journal of microbiological methods 2018 v.154 pp. 95-106
- ISSN:
- 0167-7012
- Subject:
- DNA barcoding; anthropogenic activities; automation; biodiversity; ecosystems; eukaryotic cells; filtration; genetic markers; marine sediments; microarray technology; monitoring; nucleic acid hybridization; phylogeny; remote sensing; ribosomal DNA; ribosomal RNA; sequence homology
- Abstract:
- ... There is increasing interest in finding new, more efficient methods for routine monitoring of anthropogenic effects on benthic biodiversity and ecosystems. A range of molecular methods have been developed for assessing biodiversity the last decades. Particularly interesting are microarrays targeting phylogenetic marker genes, such as the small subunit of ribosomal RNA in eukaryotes (18S rRNA). Thi ...
- DOI:
- 10.1016/j.mimet.2018.10.007
-
https://dx.doi.org/10.1016/j.mimet.2018.10.007
- Author:
- Staley, Christopher; Sadowsky, Michael J.
- Source:
- Journal of microbiological methods 2018 v.154 pp. 14-18
- ISSN:
- 0167-7012
- Subject:
- DNA; algorithms; biogeography; bioinformatics; ecosystems; genetic markers; habitats; high-throughput nucleotide sequencing; interdisciplinary research; metagenomics; microbial communities; microbiome; microorganisms; research programs; statistical analysis; technology
- Abstract:
- ... Recent advancements in metagenomic-based studies, especially analyses of amplicon-based DNA sequencing targeting taxonomic marker genes, has led to an unprecedented characterization of microbial communities from diverse ecosystems around the world. While originally constrained by a lack of appropriate analytical tools and sequencing depth, new technologies and computational and statistical algorit ...
- DOI:
- 10.1016/j.mimet.2018.09.020
-
https://dx.doi.org/10.1016/j.mimet.2018.09.020