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- Magistro, Giuseppe; Magistro, Christiane; Stief, Christian G.; Schubert, Sören
- Journal of microbiological methods 2018 v.154 pp. 25-32
- Escherichia coli; antisense RNA; bacteria; gene expression; gene silencing; genes; open reading frames; plasmids; protocols; translation (genetics)
- ... Here we present a simple and rapidly achievable protocol for gene silencing in Escherichia coli (E. coli). In this procedure, antisense RNA (asRNA) of 400-nucleotides (nt) length and absolute complementarity to the target is produced by an expression plasmid. The designed asRNA should ideally cover at least the −10 site of the promoter and the Shine-Dalgarno sequence, and additional 300-bp of the ...