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1. Rapid and efficient protocol to introduce exogenous DNA in Vibrio harveyi and Pseudoalteromonas sp.

Author:
Delavat, François; Bidault, Adeline; Pichereau, Vianney; Paillard, Christine
Source:
Journal of microbiological methods 2018 v.154 pp. 1-5
ISSN:
0167-7012
Subject:
Pseudoalteromonas; Vibrio campbellii; Vibrio harveyi; bioluminescence; electroporation; gene expression; genes; phenotypic variation; plasmids
Abstract:
... Vibrio campbellii BAA-1116 is renowned for its bioluminescence properties, and genetic tools are available to genetically track this strain. However, many other ecologically important V. harveyi strains exist, for which only few genetic tools are available. In this study, a rapid electroporation protocol was developed to transform replicative plasmids in various environmental V. harveyi and Pseudo ...
DOI:
10.1016/j.mimet.2018.09.022