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- Schriefer, Andrew E.; Cliften, Paul F.; Hibberd, Matthew C.; Sawyer, Christopher; Brown-Kennerly, Victoria; Burcea, Lauren; Klotz, Elliott; Crosby, Seth D.; Gordon, Jeffrey I.; Head, Richard D.
- Journal of microbiological methods 2018 v.154 pp. 6-13
- DNA; algorithms; bacterial communities; computer software; genes; germ-free animals; habitats; high-throughput nucleotide sequencing; metagenomics; mice; microfluidic technology; phylogeny; polymerase chain reaction; ribosomal RNA
- ... Metagenomic sequencing of bacterial samples has become the gold standard for profiling microbial populations, but 16S rRNA profiling remains widely used due to advantages in sample throughput, cost, and sensitivity even though the approach is hampered by primer bias and lack of specificity. We hypothesized that a hybrid approach, that combined targeted PCR amplification with high-throughput sequen ...
- PubMed Central:
- Hu, Jinqiang; Huang, Runna; Wang, Yi; Wei, Xiangke; Wang, Zhangcun; Geng, Yao; Jing, Jianzhou; Gao, Hui; Sun, Xincheng; Dong, Caiwen; Jiang, Chunpeng
- Journal of microbiological methods 2018 v.154 pp. 127-133
- Escherichia coli O157; Salmonella; antibodies; bacteria; beef; biotin; cabbage; chickens; color; detection limit; digoxin; enzyme-linked immunosorbent assay; food sanitation; foodborne illness; genes; juices; microbial detection; milk; monitoring; nucleic acid hybridization; polymerase chain reaction; pork; shrimp; streptomycin
- ... In the current study, a duplex PCR-ELISA method was developed targeting the specific genes, invA of Salmonella spp. and rfbE of Escherichia coli O157: H7, to detect one or both bacteria in food. In brief, PCR product amplified by PCR primer labeled with digoxin at the 5′-end and a probe labeled with biotin at the 3′-end can form dimer by nucleic acid hybridization which can be captured by binding ...