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You searched for: Journal Journal of microbiological methods Remove constraint Journal: Journal of microbiological methods Publication Year 2018 Remove constraint Publication Year: 2018 Source 2018 v.154 Remove constraint Source: 2018 v.154 Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
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1. Antibacterial activity of lysozyme-binding proteins from chicken egg white

Author:
Shimazaki, Youji; Takahashi, Ayumi
Source:
Journal of microbiological methods 2018 v.154 pp. 19-24
ISSN:
0167-7012
Subject:
Bacillus subtilis; Escherichia coli; Gram-negative bacteria; antibacterial properties; cathodes; digital images; egg albumen; isoelectric focusing; lysozyme; ovalbumin; phosphoric acid; sodium hydroxide; two-dimensional gel electrophoresis
Abstract:
... The purpose of this study was to establish a method for determining the bacteriolytic activity after separation of lysozyme-binding proteins from egg white. Lysozyme-binding proteins such as ovotransferrin and ovalbumin were separated by non-denaturing two-dimensional electrophoresis (2DE) and transferred to a membrane. The lysozyme activity of the separated and immobilized egg white proteins was ...
DOI:
10.1016/j.mimet.2018.10.001

2. Application of Cytosense flow cytometer for the analysis of airborne bacteria collected by a high volume impingement sampler

Author:
Jang, Jaeyoun; Hendriksen, Niels Bohse; Jakobsen, Hans H.; Gosewinkel, Ulrich
Source:
Journal of microbiological methods 2018 v.154 pp. 63-72
ISSN:
0167-7012
Subject:
DNA; air; airborne microorganisms; bacteria; flow cytometry; genes; quantitative polymerase chain reaction; regression analysis; relative humidity; ribosomal RNA; wastewater treatment
Abstract:
... Characterization of airborne bacterial cells requires efficient collection, concentration, and analysis techniques, particularly to overcome the challenge of their dilute nature in outdoor environments. This study aims to establish a rapid and reliable approach for quantification of bacteria in air samples. To do this, a high volume impingement sampler was applied to collect airborne bacteria from ...
DOI:
10.1016/j.mimet.2018.10.012

3. A simple and highly efficient method for gene silencing in Escherichia coli

Author:
Magistro, Giuseppe; Magistro, Christiane; Stief, Christian G.; Schubert, Sören
Source:
Journal of microbiological methods 2018 v.154 pp. 25-32
ISSN:
0167-7012
Subject:
Escherichia coli; antisense RNA; bacteria; gene expression; gene silencing; genes; open reading frames; plasmids; protocols; translation (genetics)
Abstract:
... Here we present a simple and rapidly achievable protocol for gene silencing in Escherichia coli (E. coli). In this procedure, antisense RNA (asRNA) of 400-nucleotides (nt) length and absolute complementarity to the target is produced by an expression plasmid. The designed asRNA should ideally cover at least the −10 site of the promoter and the Shine-Dalgarno sequence, and additional 300-bp of the ...
DOI:
10.1016/j.mimet.2018.10.003

4. A simplified pyrazinamidase test for pyrazinamide drug susceptibility in Mycobacterium tuberculosis

Author:
Aono, Akio; Chikamatsu, Kinuyo; Yamada, Hiroyuki; Igarashi, Yuriko; Murase, Yoshiro; Takaki, Akiko; Mitarai, Satoshi
Source:
Journal of microbiological methods 2018 v.154 pp. 52-54
ISSN:
0167-7012
Subject:
Mycobacterium tuberculosis; drugs; enzyme activity; enzymes; liquids; phenotype
Abstract:
... We modified Wayne's pyrazinamidase test against Mycobacterium tuberculosis to indirectly measure pyrazinamidase activity via pyrazinoic acid in liquid medium. The modified pyrazinamidase test was easy to perform and its results were in complete agreement with those of the conventional Wayne's method, highlighting its potential application in phenotypic pyrazinamide susceptibility testing. ...
DOI:
10.1016/j.mimet.2018.09.018

5. Detection of Tilletia caries, Tilletia laevis and Tilletia controversa wheat grain contamination using loop-mediated isothermal DNA amplification (LAMP)

Author:
Pieczul, Katarzyna; Perek, Agnieszka; Kubiak, Krzysztof
Source:
Journal of microbiological methods 2018 v.154 pp. 141-146
ISSN:
0167-7012
Subject:
Alternaria; Cladosporium; DNA; Fusarium; Helminthosporium; Penicillium; Tilletia controversa; Tilletia laevis; Tilletia tritici; detection limit; fungi; protocols; rapid methods; smut diseases; teliospores; wheat
Abstract:
... The study describes a novel diagnostic protocol based on a loop-mediated isothermal DNA amplification (LAMP) for identification of wheat grains infection by Tilletia laevis, Tilletia caries (common bunt) and Tilletia controversa (draft bunt). The presented data showed that the LAMP analysis is a simple, specific and rapid method that could be used for detection of Tilletia spp. in contaminated gra ...
DOI:
10.1016/j.mimet.2018.10.018

6. Determination of live:dead bacteria as a function of antibiotic treatment

Author:
Li, Runze; Dhankhar, Dinesh; Chen, Jie; Cesario, Thomas C.; Rentzepis, Peter M.
Source:
Journal of microbiological methods 2018 v.154 pp. 73-78
ISSN:
0167-7012
Subject:
DNA; antibiotic resistance; antibiotics; bacteria; drugs; fluorescence emission spectroscopy; patients; principal component analysis; tryptophan; tyrosine
Abstract:
... Antibiotics are drugs that react against, kill, or inhibit the growth of bacteria. The method most often employed to evaluate the effectiveness of an antibiotic to kill bacteria requires at least 16 to 24 h for bacterial incubation. The requirement of long periods of time for the determination of the number of bacteria still alive after antibiotic treatment, may, in many cases, be detrimental to t ...
DOI:
10.1016/j.mimet.2018.10.010

7. Development and testing of an 18S rRNA phylogenetic microarray for marine sediments

Author:
Lekang, Katrine; Hadziavdic, Kenan; Sandnes Skaar, Katrine; Jonassen, Inge; Thompson, Eric M.; Troedsson, Christofer
Source:
Journal of microbiological methods 2018 v.154 pp. 95-106
ISSN:
0167-7012
Subject:
DNA barcoding; anthropogenic activities; automation; biodiversity; ecosystems; eukaryotic cells; filtration; genetic markers; marine sediments; microarray technology; monitoring; nucleic acid hybridization; phylogeny; remote sensing; ribosomal DNA; ribosomal RNA; sequence homology
Abstract:
... There is increasing interest in finding new, more efficient methods for routine monitoring of anthropogenic effects on benthic biodiversity and ecosystems. A range of molecular methods have been developed for assessing biodiversity the last decades. Particularly interesting are microarrays targeting phylogenetic marker genes, such as the small subunit of ribosomal RNA in eukaryotes (18S rRNA). Thi ...
DOI:
10.1016/j.mimet.2018.10.007

8. Development of a high throughput optical density assay to determine fungicide sensitivity of oomycetes

Author:
Hunter, Shannon; McDougal, Rebecca; Clearwater, Mike J.; Williams, Nari; Scott, Peter
Source:
Journal of microbiological methods 2018 v.154 pp. 33-39
ISSN:
0167-7012
Subject:
Phytophthora cinnamomi; absorbance; biological resistance; fungicides; growth retardation; inventories; median effective concentration; mycelium
Abstract:
... A high-throughput assay was developed to screen Phytophthora species for fungicide sensitivity using optical density measurements for unbiased, automated measurement of mycelial growth. The efficacy of the optical density assay (OD) to measure phosphite sensitivity in Phytophthora species was compared to two widely used methods, radial growth (RG) and dry weight (DW) assays. Three isolates of each ...
DOI:
10.1016/j.mimet.2018.10.006

9. Development of duplex PCR-ELISA for simultaneous detection of Salmonella spp. and Escherichia coli O157: H7 in food

Author:
Hu, Jinqiang; Huang, Runna; Wang, Yi; Wei, Xiangke; Wang, Zhangcun; Geng, Yao; Jing, Jianzhou; Gao, Hui; Sun, Xincheng; Dong, Caiwen; Jiang, Chunpeng
Source:
Journal of microbiological methods 2018 v.154 pp. 127-133
ISSN:
0167-7012
Subject:
Escherichia coli O157; Salmonella; antibodies; bacteria; beef; biotin; cabbage; chickens; color; detection limit; digoxin; enzyme-linked immunosorbent assay; food sanitation; foodborne illness; genes; juices; microbial detection; milk; monitoring; nucleic acid hybridization; polymerase chain reaction; pork; shrimp; streptomycin
Abstract:
... In the current study, a duplex PCR-ELISA method was developed targeting the specific genes, invA of Salmonella spp. and rfbE of Escherichia coli O157: H7, to detect one or both bacteria in food. In brief, PCR product amplified by PCR primer labeled with digoxin at the 5′-end and a probe labeled with biotin at the 3′-end can form dimer by nucleic acid hybridization which can be captured by binding ...
DOI:
10.1016/j.mimet.2018.10.017

10. Direct diagnosis of Brucella species through multiplex PCR formed by a new method

Author:
Saytekin, Ahmet Murat; Ak, Seyyal
Source:
Journal of microbiological methods 2018 v.154 pp. 86-94
ISSN:
0167-7012
Subject:
Brucella; brucellosis; fetus; liver; lungs; polymerase chain reaction; ruminants; stomach
Abstract:
... This study aimed to develop direct PCR methods, which enable the diagnosis of brucellosis agents from ruminant aborted fetus samples at species and genus levels, and determine the applicability of the newly developed methods. For this purpose, 137 lung, 137 liver, and 52 fetal stomach fluid samples belonging to 166 ruminant aborted fetuses (326 samples in total) were examined. Firstly, agent isola ...
DOI:
10.1016/j.mimet.2018.10.011

11. Enrichment of semen culture in the diagnosis of bacterial prostatitis

Author:
Iovene, Maria Rosaria; Martora, Francesca; Mallardo, Enza; De Sio, Marco; Arcaniolo, Davide; Del Vecchio, Chiara; Pagliuca, Chiara; Signoriello, Giuseppe; Vitiello, Mariateresa
Source:
Journal of microbiological methods 2018 v.154 pp. 124-126
ISSN:
0167-7012
Subject:
bacterial infections; prostatitis; protocols; semen
Abstract:
... The objective was to investigate the diagnostic accuracy of our microbiological protocol to simplify the evaluation of bacterial prostatitis in the clinical practice.Our findings show the possibility to apply our alternative enrichment semen culture method to detect prostatic bacterial infection with higher sensitivity than the gold standard M&S technique. ...
DOI:
10.1016/j.mimet.2018.10.016

12. Evaluation of modified saponin preparation method for the direct identification and antimicrobial susceptibility testing from positive blood culture

Author:
Lee, Ji Eun; Jo, Sung Jin; Park, Kang Gyun; Suk, Hyun Soo; Ha, Sung Il; Shin, Jeong Seob; Park, Yeon-Joon
Source:
Journal of microbiological methods 2018 v.154 pp. 118-123
ISSN:
0167-7012
Subject:
Campylobacter; Streptococcus pneumoniae; anaerobes; antibiotic resistance; bacteria; blood; bottles; diagnostic techniques; matrix-assisted laser desorption-ionization mass spectrometry; pellets; saponins; yeasts
Abstract:
... We evaluated the two in-house sample preparation methods (saponin method (SAP) and [saponin + Sputazyme] method (SSPZ)) for direct identification of microorganisms using MALDI-TOF MS from positive blood culture bottles. Also, we evaluated the [saponin + Sputazyme] method for direct antimicrobial susceptibility testing (AST) using Vitek 2 system.For direct identification, 163 prospective, monomicro ...
DOI:
10.1016/j.mimet.2018.10.004

13. Identification of Escherichia coli strains for the heterologous overexpression of soluble Clostridium difficile exosporium proteins

Author:
Brito-Silva, Christian; Pizarro-Cerda, Jaime; Gil, Fernando; Paredes-Sabja, Daniel
Source:
Journal of microbiological methods 2018 v.154 pp. 46-51
ISSN:
0167-7012
Subject:
Clostridium difficile; Escherichia coli; exine; molecular biology; morphs; recombinant proteins; spores
Abstract:
... Clostridium difficile infections are one of the leading causes of hospital-acquired infections. C. difficile spores are considered the morphotype of transmission and recurrent infection due to its natural spore resistance properties. The outermost spore layer, the exosporium, provides the first contact with the environment and the host. However, molecular biology studies on exosporium proteins are ...
DOI:
10.1016/j.mimet.2018.10.002

14. Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions

Author:
Šedo, Ondrej; Radolfová-Křížová, Lenka; Nemec, Alexandr; Zdráhal, Zbyněk
Source:
Journal of microbiological methods 2018 v.154 pp. 79-85
ISSN:
0167-7012
Subject:
Acinetobacter haemolyticus; Acinetobacter junii; Acinetobacter venetianus; databases; ferulic acid; matrix-assisted laser desorption-ionization mass spectrometry; phylogeny
Abstract:
... A set of 204 taxonomically well-defined strains belonging to 17 Acinetobacter spp., including 11 recently described species (A. albensis, A. bohemicus, A. colistiniresistens, A. courvalinii. A. dispersus, A. gandensis, A. modestus, A. proteolyticus, A. seifertii, A. variabilis, and A. vivianii) and six species of the so-called haemolytic clade (A. beijerinckii, A. gyllenbergii, A. haemolyticus, A. ...
DOI:
10.1016/j.mimet.2018.10.009

15. Method for isolation of both lactose-fermenting and – non-fermenting Escherichia albertii strains from stool samples

Author:
Maheux, Andrée F.; Brodeur, Stéphanie; Bérubé, Ève; Boudreau, Dominique K.; Abed, Jehane Y.; Boissinot, Maurice; Bissonnette, Luc; Bergeron, Michel G.
Source:
Journal of microbiological methods 2018 v.154 pp. 134-140
ISSN:
0167-7012
Subject:
Escherichia albertii; bacteria; feces; fermentation; isolation techniques; lactose; pathogenicity; phenotype; polymerase chain reaction; species identification
Abstract:
... Initially, Escherichia albertii has been described as a non-lactose fermenting bacterium and methods used to isolate it were first based on this phenotypic property. However, a recent study showed a variable lactose fermentation phenotype for E. albertii suggesting that this microorganism could have been underestimated by previous studies using isolation methods based on lactose fermentation. In t ...
DOI:
10.1016/j.mimet.2018.09.008

16. Miniaturized whole-cell bacterial bioreporter assay for identification of quorum sensing interfering compounds

Author:
Ilina, Polina; Ma, Xiaochu; Sintim, Herman O.; Tammela, Päivi
Source:
Journal of microbiological methods 2018 v.154 pp. 40-45
ISSN:
0167-7012
Subject:
Escherichia coli; antibiotic resistance; bacteria; bacterial infections; beta-galactosidase; pathogenicity; quorum sensing
Abstract:
... The continuing emergence and spread of antibiotic-resistant bacteria is worrisome and new strategies to curb bacterial infections are being sought. The interference of bacterial quorum sensing (QS) signaling has been suggested as a prospective antivirulence strategy. The AI-2 QS system is present in multiple bacterial species and has been shown to be correlated with pathogenicity. To facilitate th ...
DOI:
10.1016/j.mimet.2018.10.005

17. Over expression of the constitutive expression refractory Mycobacterium tuberculosis Rv3488 protein with an acid-inducible expression system

Author:
Pham, Nam; Dao, Nelson; Saviola, Beatrice
Source:
Journal of microbiological methods 2018 v.154 pp. 112-117
ISSN:
0167-7012
Subject:
Mycobacterium smegmatis; Mycobacterium tuberculosis; carotenoids; cell growth; gene overexpression; genes; macrophages; pH; phagosomes; plasmids; protein synthesis; proteins
Abstract:
... As inducible protein expression plasmids available for mycobacterial species are limited, here we demonstrate the utility of an acid-inducible promoter driving gene and subsequent protein expression of a difficult to express protein. We wanted to assess the use of an acid inducible promoter expression system to produce proteins in a mycobacterial system, specifically proteins that when overexpress ...
DOI:
10.1016/j.mimet.2018.09.021

18. Practical considerations for sampling and data analysis in contemporary metagenomics-based environmental studies

Author:
Staley, Christopher; Sadowsky, Michael J.
Source:
Journal of microbiological methods 2018 v.154 pp. 14-18
ISSN:
0167-7012
Subject:
DNA; algorithms; biogeography; bioinformatics; ecosystems; genetic markers; habitats; high-throughput nucleotide sequencing; interdisciplinary research; metagenomics; microbial communities; microbiome; microorganisms; research programs; statistical analysis; technology
Abstract:
... Recent advancements in metagenomic-based studies, especially analyses of amplicon-based DNA sequencing targeting taxonomic marker genes, has led to an unprecedented characterization of microbial communities from diverse ecosystems around the world. While originally constrained by a lack of appropriate analytical tools and sequencing depth, new technologies and computational and statistical algorit ...
DOI:
10.1016/j.mimet.2018.09.020

19. Rapid and efficient protocol to introduce exogenous DNA in Vibrio harveyi and Pseudoalteromonas sp.

Author:
Delavat, François; Bidault, Adeline; Pichereau, Vianney; Paillard, Christine
Source:
Journal of microbiological methods 2018 v.154 pp. 1-5
ISSN:
0167-7012
Subject:
Pseudoalteromonas; Vibrio campbellii; Vibrio harveyi; bioluminescence; electroporation; gene expression; genes; phenotypic variation; plasmids
Abstract:
... Vibrio campbellii BAA-1116 is renowned for its bioluminescence properties, and genetic tools are available to genetically track this strain. However, many other ecologically important V. harveyi strains exist, for which only few genetic tools are available. In this study, a rapid electroporation protocol was developed to transform replicative plasmids in various environmental V. harveyi and Pseudo ...
DOI:
10.1016/j.mimet.2018.09.022

20. Rapid detection of Yersinia enterocolitica serotype O:3 using a duplex PCR assay

Author:
Rusak, Leonardo Alves; de Castro Lisboa Pereira, Rodrigo; Freitag, Isabelle Geoffroy; Hofer, Cristina Barroso; Hofer, Ernesto; Asensi, Marise Dutra; Vallim, Deyse Christina
Source:
Journal of microbiological methods 2018 v.154 pp. 107-111
ISSN:
0167-7012
Subject:
DNA; Yersinia enterocolitica; biosynthesis; digestive system diseases; feces; hospitals; humans; monitoring; peptide elongation factors; polymerase chain reaction; pregnant women; public health; rapid methods; selective media; serotypes; Europe
Abstract:
... Yersinia enterocolitica, a member of the Enterobacteriaceae family, is a zoonotic agent that causes gastrointestinal diseases and some extraintestinal disorders in humans. Y. enterocolitica ssp. palearctica bioserotype 4/O:3 is the primary pathogenic bioserotype in Europe, where it has a high public health relevance. The isolation and identification of Y. enterocolitica from various sources on sel ...
DOI:
10.1016/j.mimet.2018.10.014

21. Rapid differentiation of Lactobacillus species via metabolic profiling

Author:
Yang, Kundi; Xu, Mengyang; Zhong, Fanyi; Zhu, Jiangjiang
Source:
Journal of microbiological methods 2018 v.154 pp. 147-155
ISSN:
0167-7012
Subject:
Lactobacillus; data quality; digestive system; functional properties; humans; lactic acid; lactic acid bacteria; metabolites; metabolomics; monitoring; probiotics; spectrometers; sugars
Abstract:
... Lactobacillus, the major genus of lactic acid bacteria group, plays functional roles in the human body, for example, convert sugars to lactic acid. They are the significant microbiota which can be found at a number of human body sites, such as the digestive system, urinary system, and genital system. A number of Lactobacillus species are often used as probiotics and can benefit host health when ad ...
DOI:
10.1016/j.mimet.2018.10.013