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You searched for: Journal Journal of microbiological methods Remove constraint Journal: Journal of microbiological methods Publication Year 2016 Remove constraint Publication Year: 2016 Source 2016 v.125 Remove constraint Source: 2016 v.125
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1. A quick colorimetric method for total lipid quantification in microalgae

Author:
Byreddy, Avinesh R.; Gupta, Adarsha; Barrow, Colin J.; Puri, Munish
Source:
Journal of microbiological methods 2016 v.125 pp. 28-32
ISSN:
0167-7012
Subject:
biodiesel; colorimetry; fuel production; glycerol; gravimetry; lipids; microalgae; proteins; quantitative analysis; screening; vegetable oil
Abstract:
... Discovering microalgae with high lipid productivity are among the key milestones for achieving sustainable biodiesel production. Current methods of lipid quantification are time intensive and costly. A rapid colorimetric method based on sulfo-phospho-vanillin (SPV) reaction was developed for the quantification of microbial lipids to facilitate screening for lipid producing microalgae. This method ...
DOI:
10.1016/j.mimet.2016.04.002

2. A series of template plasmids for Escherichia coli genome engineering

Author:
Deb, Shalini S.; Reshamwala, Shamlan M.S.; Lali, Arvind M.
Source:
Journal of microbiological methods 2016 v.125 pp. 49-57
ISSN:
0167-7012
Subject:
Escherichia coli; antibiotics; genes; genetic engineering; metabolic engineering; plasmids; promoter regions
Abstract:
... Metabolic engineering strategies often employ multi-copy episomal vectors to overexpress genes. However, chromosome-based overexpression is preferred as it avoids the use of selective pressure and reduces metabolic burden on the cell. We have constructed a series of template plasmids for λ Red-mediated Escherichia coli genome engineering. The template plasmids allow construction of genome integrat ...
DOI:
10.1016/j.mimet.2016.04.006

3. BEEP: An assay to detect bio-energetic and envelope permeability alterations in Pseudomonas aeruginosa

Author:
Suh, Sang-Jin; Shuman, Jon; Carroll, Leslie P.; Silo-Suh, Laura
Source:
Journal of microbiological methods 2016 v.125 pp. 81-86
ISSN:
0167-7012
Subject:
Pseudomonas aeruginosa; adenosine triphosphate; antibiotics; bacteria; energy metabolism; genes; luciferase; membrane permeability; mutants; mutation; phenotype; transposons
Abstract:
... We developed an effective and rapid assay to detect both bio-energetic and envelope permeability (BEEP) alterations of Pseudomonas aeruginosa. The assay is based on quantification of extracellular ATP in bacterial cultures using luciferase as a reporter. To demonstrate the validity of our assay we conducted a biased screen of a transposon insertion library in P. aeruginosa strain PAO1 in order to ...
DOI:
10.1016/j.mimet.2016.04.009
Chorus Open Access:
10.1016/j.mimet.2016.04.009

4. Development of a gene delivery system in Streptococcus gordonii using thymidylate synthase as a selection marker

Author:
Lee, Song F.; Hulbah, Maram; Halperin, Scott A.
Source:
Journal of microbiological methods 2016 v.125 pp. 43-48
ISSN:
0167-7012
Subject:
Bordetella pertussis; DNA fragmentation; Streptococcus gordonii; antibodies; antigens; bacteria; chromosomes; complement; crossing over; gene transfer; genes; hemagglutinins; humans; immune response; live vaccines; mice; mouth; mutants; polymerase chain reaction; thymidine; thymidylate synthase
Abstract:
... Streptococcus gordonii, a commensal bacterium of the human oral cavity, is a potential live vaccine vector. In this study, we have developed a system that delivers a vaccine antigen gene onto the chromosome of S. gordonii. The system consisted of a recipient strain, that is a thymidine auxotroph constructed by deletion of a portion of thyA gene, and a linear gene delivery construct, composed of th ...
DOI:
10.1016/j.mimet.2016.04.003

5. Discriminative power of Campylobacter phenotypic and genotypic typing methods

Author:
Duarte, Alexandra; Seliwiorstow, Tomasz; Miller, William G.; De Zutter, Lieven; Uyttendaele, Mieke; Dierick, Katelijne; Botteldoorn, Nadine
Source:
Journal of microbiological methods 2016 v.125 pp. 33-39
ISSN:
0167-7012
Subject:
Campylobacter coli; Campylobacter jejuni; antibiotic resistance; chicken meat; flagellin; genes; lipooligosaccharides; meat carcasses; monitoring; multilocus sequence typing; phenotype; restriction fragment length polymorphism; virulence
Abstract:
... The aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of Campylobacter in food. Campylobacter jejuni (n=94) and Campylobacter coli (n=52) isolated from different broiler meat carcasses were characterized using multilocus sequence typing (MLST), flagellin gene A restriction fragment length polymorphism typing (flaA-RFLP), antimicrobial r ...
DOI:
10.1016/j.mimet.2016.03.004

6. Evaluation of Ag containing hydroxyapatite coatings to the Candida albicans infection

Author:
Ciuca, S.; Badea, M.; Pozna, E.; Pana, I.; Kiss, A.; Floroian, L.; Semenescu, A.; Cotrut, C.M.; Moga, M.; Vladescu, A.
Source:
Journal of microbiological methods 2016 v.125 pp. 12-18
ISSN:
0167-7012
Subject:
Candida albicans; X-ray diffraction; alloys; antifungal properties; coatings; hydroxyapatite; prostheses; roughness; silver
Abstract:
... In this research work, the synthesis of Ag doped hydroxyapatite coatings for dental or orthopedic implants was performed. The main goal was to determine the influence of Ag content on the roughness and antimicrobial performance of the prepared thin films. The films were deposited on Ti6Al4V alloy by means of RF magnetron sputtering. Those coatings were characterized by X-ray diffraction (XRD) and ...
DOI:
10.1016/j.mimet.2016.03.016

7. Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth

Author:
Dias, Kássia de Carvalho; Barbugli, Paula Aboud; Vergani, Carlos Eduardo
Source:
Journal of microbiological methods 2016 v.125 pp. 40-42
ISSN:
0167-7012
Subject:
buffers; cell viability; coculture; glutamine; keratinocytes; microbial growth; sodium bicarbonate
Abstract:
... This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3-(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with l-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture. ...
DOI:
10.1016/j.mimet.2016.03.018

8. Integrative bacterial artificial chromosomes for DNA integration into the Bacillus subtilis chromosome

Author:
Juhas, Mario; Ajioka, James W.
Source:
Journal of microbiological methods 2016 v.125 pp. 1-7
ISSN:
0167-7012
Subject:
Bacillus subtilis; DNA; DNA assembly; DNA fragmentation; Escherichia coli K12; antibiotic resistance; bacteria; bacterial artificial chromosomes; biotechnology; genetic engineering; homologous recombination; loci; synthetic biology; yellow fluorescent protein
Abstract:
... Bacillus subtilis is a well-characterized model bacterium frequently used for a number of biotechnology and synthetic biology applications. Novel strategies combining the advantages of B. subtilis with the DNA assembly and editing tools of Escherichia coli are crucial for B. subtilis engineering efforts. We combined Gibson Assembly and λ red recombineering in E. coli with RecA-mediated homologous ...
DOI:
10.1016/j.mimet.2016.03.017

9. Multiple-locus variable-number tandem repeat analysis for strain discrimination of non-O157 Shiga toxin-producing Escherichia coli

Author:
Timmons, Chris; Trees, Eija; Ribot, Efrain M.; Gerner-Smidt, Peter; LaFon, Patti; Im, Sung; Ma, Li Maria
Source:
Journal of microbiological methods 2016 v.125 pp. 70-80
ISSN:
0167-7012
Subject:
Shiga toxin-producing Escherichia coli; food contamination; food pathogens; foodborne illness; loci; minisatellite repeats; multiple-locus variable number tandem-repeat analysis; outbreak investigation; polymerase chain reaction; pulsed-field gel electrophoresis; serotypes; tandem repeat sequences
Abstract:
... Non-O157 Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens of growing concern worldwide that have been associated with several recent multistate and multinational outbreaks of foodborne illness. Rapid and sensitive molecular-based bacterial strain discrimination methods are critical for timely outbreak identification and contaminated food source traceback. One such method, mult ...
DOI:
10.1016/j.mimet.2016.04.005
Chorus Open Access:
10.1016/j.mimet.2016.04.005

10. Novel cost-effective quality control approach for the Cepheid Xpert CT/NG assay for the detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae

Author:
Chan, Maurice; Jiang, Boran; Ng, Siew Yong Lily; Tan, Thean Yen
Source:
Journal of microbiological methods 2016 v.125 pp. 87-90
ISSN:
0167-7012
Subject:
Chlamydia trachomatis; Neisseria gonorrhoeae; cost effectiveness; eyes; humans; quality control
Abstract:
... The Xpert CT/NG is a rapid assay for detection of Neisseria gonorrhoeae and Chlamydia trachomatis. QC materials must be formulated to emulate human specimens, and are prohibitively expensive. A creative, cost-effective QC approach is proposed. The acceptable sample types for the Xpert CT/NG assay were extended to include eye swabs. ...
DOI:
10.1016/j.mimet.2016.04.010

11. Novel oligonucleotide primers reveal a high diversity of microbes which drive phosphorous turnover in soil

Author:
Bergkemper, Fabian; Kublik, Susanne; Lang, Friederike; Krüger, Jaane; Vestergaard, Gisle; Schloter, Michael; Schulz, Stefanie
Source:
Journal of microbiological methods 2016 v.125 pp. 91-97
ISSN:
0167-7012
Subject:
DNA; DNA primers; Fagus; acid phosphatase; alkaline phosphatase; bioavailability; ecosystems; forest soils; fungi; genes; glucose; metagenomics; microbial communities; mineralization; phosphorus; phytases; solubilization; spatial variation; transporters
Abstract:
... Phosphorus (P) is of central importance for cellular life but likewise a limiting macronutrient in numerous environments. Certainly microorganisms have proven their ability to increase the phosphorus bioavailability by mineralization of organic-P and solubilization of inorganic-P. On the other hand they efficiently take up P and compete with other biota for phosphorus. However the actual microbial ...
DOI:
10.1016/j.mimet.2016.04.011

12. Optimisation of a droplet digital PCR assay for the diagnosis of Schistosoma japonicum infection: A duplex approach with DNA binding dye chemistry

Author:
Weerakoon, Kosala G.; Gordon, Catherine A.; Gobert, Geoffrey N.; Cai, Pengfei; McManus, Donald P.
Source:
Journal of microbiological methods 2016 v.125 pp. 19-27
ISSN:
0167-7012
Subject:
DNA; Schistosoma japonicum; adults; chemistry; detection limit; diagnostic techniques; dye binding; eggs; genes; mitochondria; parasites; polymerase chain reaction; public health; retrotransposons; schistosomiasis; socioeconomics
Abstract:
... Schistosomiasis is a chronically debilitating helminth infection with a significant socio-economic and public health impact. Accurate diagnostics play a pivotal role in achieving current schistosomiasis control and elimination goals. However, many of the current diagnostic procedures, which rely on detection of schistosome eggs, have major limitations including lack of accuracy and the inability t ...
DOI:
10.1016/j.mimet.2016.03.012

13. Simple method for correct enumeration of Staphylococcus aureus

Author:
Haaber, J.; Cohn, M.T.; Petersen, A.; Ingmer, H.
Source:
Journal of microbiological methods 2016 v.125 pp. 58-63
ISSN:
0167-7012
Subject:
Staphylococcus aureus; absorbance; agar; bacteria; humans; pathogens
Abstract:
... Optical density (OD) measurement is applied universally to estimate cell numbers of microorganisms growing in liquid cultures. It is a fast and reliable method but is based on the assumption that the bacteria grow as single cells of equal size and that the cells are dispersed evenly in the liquid culture. When grown in such liquid cultures, the human pathogen Staphylococcus aureus is characterized ...
DOI:
10.1016/j.mimet.2016.04.004

14. Simultaneous detection of Waddlia chondrophila and Listeria monocytogenes in aborted ruminant samples by real-time quantitative PCR

Author:
Barkallah, Mohamed; Gharbi, Yaakoub; Slima, Ahlem Ben; Elleuch, Fatma; Mallek, Zouhir; Saad, Rania Ben; Gautier, Michel; Gdoura, Radhouane; Fendri, Imen
Source:
Journal of microbiological methods 2016 v.125 pp. 64-69
ISSN:
0167-7012
Subject:
Listeria monocytogenes; Waddlia chondrophila; animal tissues; bacteria; blood; detection limit; milk; pathogens; placenta; plasmids; quantitative polymerase chain reaction; ruminants; screening
Abstract:
... Waddlia chondrophila and Listeria monocytogenes are well known emerging pathogens that cause ruminants' abortion around the world. Zoonotic infections caused by these bacteria are mostly underestimated due to difficulties of diagnosis resulting from their intracellular growth. The purpose of this study was to develop and validate a dual real-time quantitative PCR (qPCR) assay for the simultaneous ...
DOI:
10.1016/j.mimet.2016.04.001

15. The identification of carbapenemase types in Enterobacteriaceae by using molecular assay and phenotyping confirmation tests

Author:
Genc, Ozlem; Aksu, Evrim; Gulcan, Aynur
Source:
Journal of microbiological methods 2016 v.125 pp. 8-11
ISSN:
0167-7012
Subject:
EDTA (chelating agent); Enterobacteriaceae; antibiotic resistance; beta-lactamase; cloxacillin; exercise test; genes; phenotype; screening
Abstract:
... In this study, we aimed to identify the molecular carbapenemase types of the Enterobacteriaceae isolates and to evaluate the performance of manually prepared and commercially available combination disc methods and the modified Hodge test. One hundred and forty carbapenemase producing isolates and 45 isolates as control group were included in our study. The Xpert CARBA-R test was used as the molecu ...
DOI:
10.1016/j.mimet.2016.03.010