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You searched for: Journal name Journal of microbiological methods Remove constraint Journal name: Journal of microbiological methods Publication year rev 7986-2014 Remove constraint Publication year rev: 7986-2014 Source 2014 v.103 Remove constraint Source: 2014 v.103
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1. Clinical evaluation of a disposable amperometric magneto-genosensor for the detection and identification of Streptococcus pneumoniae

Author:
Alma Sotillo; María Pedrero; Manuela de Pablos; José Luis García; Ernesto García; Pedro García; José Manuel Pingarrón; Jesús Mingorance; Susana Campuzano
Source:
Journal of microbiological methods 2014 v.103 pp. 25-28
ISSN:
0167-7012
Subject:
Streptococcus pneumoniae; clinical examination; polymerase chain reaction
Abstract:
... A disposable PCR-based amperometric magneto-genosensor for detection and identification of Streptococcus pneumoniae was evaluated. ROC curve analysis used to determine optimal signal cutoff values yielded a sensitivity of 91% and a specificity of 90%. The method was also tested for the direct detection of pneumococci in clinical samples. ...
DOI:
10.1016/j.mimet.2014.04.014

2. Development of an oriC vector for use in Mycoplasma synoviae

Author:
Muhammad A. Shahid; Marc S. Marenda; Philip F. Markham; Amir H. Noormohammadi
Source:
Journal of microbiological methods 2014 v.103 pp. 70-76
ISSN:
0167-7012
Subject:
Mycoplasma synoviae; egg production; financial economics; gene targeting; genetic engineering; genetic vectors; homologous recombination; pathogens; plasmids; poultry; replication origin; resistance genes; synovitis; tetracycline; virulence
Abstract:
... Mycoplasma synoviae, an important poultry pathogen, belonging to the class Mollicutes, causes airsacculitis, synovitis, decreased egg production and produces significant economic losses. Efforts to determine M. synoviae virulence factors and their role in pathogenicity require suitable tools for genetic manipulation of this pathogen. This study describes, for the first time, the identification and ...
DOI:
10.1016/j.mimet.2014.05.014

3. Efficient construction of unmarked recombinant mycobacteria using an improved system

Author:
Feng Yang; Yaoju Tan; Jia Liu; Tianzhou Liu; Bangxing Wang; Yuanyuan Cao; Yue Qu; Trevor Lithgow; Shouyong Tan; Tianyu Zhang
Source:
Journal of microbiological methods 2014 v.103 pp. 29-36
ISSN:
0167-7012
Subject:
Mycobacterium tuberculosis; Mycobacterium ulcerans; antibiotics; mutants
Abstract:
... The genetic study of mycobacteria, such as Mycobacterium tuberculosis and Mycobacterium ulcerans, is hampered heavily by their slow growth. We have developed efficient, versatile, and improved genetic tools for constructing unmarked recombinant mycobacteria more rapidly including generating multiple mutants using the same antibiotic marker in both fast- and slow-growing mycobacteria. ...
DOI:
10.1016/j.mimet.2014.05.007

4. Evaluation of viability-qPCR detection system on viable and dead Salmonella serovar Enteritidis

Author:
Elodie Barbau-Piednoir; Jacques Mahillon; Julie Pillyser; Wim Coucke; Nancy H. Roosens; Nadine Botteldoorn
Source:
Journal of microbiological methods 2014 v.103 pp. 131-137
ISSN:
0167-7012
Subject:
Salmonella; bacteria; food pathogens; microbial detection; propidium; protocols; quantitative polymerase chain reaction; serotypes; viability
Abstract:
... The propidium monoazide (PMA) coupled with PCR (viability PCR) is used in foodborne pathogen detection in order to detect only viable bacteria. Originally presented to fully remove the signal of dead bacteria, the limits of the viability PCR rapidly came out in the literature. In this study, the use of PMA in a viability-qPCR (v-qPCR) was assessed on viable and dead cells of Salmonella enterica su ...
DOI:
10.1016/j.mimet.2014.06.003

5. Multispecies biofilm in an artificial wound bed—A novel model for in vitro assessment of solid antimicrobial dressings

Author:
J. Kucera; M. Sojka; V. Pavlik; K. Szuszkiewicz; V. Velebny; P. Klein
Source:
Journal of microbiological methods 2014 v.103 pp. 18-24
ISSN:
0167-7012
Subject:
Bacillus subtilis; Enterococcus faecalis; Pseudomonas aeruginosa; Staphylococcus aureus; agar; antibiotics; bacteria; biofilm; cost effectiveness; in vitro studies; iodine; models; patients
Abstract:
... Wound infections represent a major problem, particularly in patients with chronic wounds. Bacteria in the wound exist mainly in the form of biofilms and are thus resistant to most antibiotics and antimicrobials. A simple and cost-effective in vitro model of chronic wound biofilms applied for testing treatments and solid devices, especially wound dressings, is presented in this work. The method is ...
DOI:
10.1016/j.mimet.2014.05.008

6. Selection of unique Escherichia coli clones by random amplified polymorphic DNA (RAPD): Evaluation by whole genome sequencing

Author:
Karen L. Nielsen; Paul A. Godfrey; Marc Stegger; Paal S. Andersen; Michael Feldgarden; Niels Frimodt-Møller
Source:
Journal of microbiological methods 2014 v.103 pp. 101-103
ISSN:
0167-7012
Subject:
Escherichia coli; clones; intestinal microorganisms; random amplified polymorphic DNA technique; screening; sequence analysis
Abstract:
... Identifying and characterizing clonal diversity are important when analysing fecal flora. We evaluated random amplified polymorphic DNA (RAPD) PCR, applied for selection of Escherichia coli isolates, by whole genome sequencing. RAPD was fast, and reproducible as screening method for selection of distinct E. coli clones in fecal swabs. ...
DOI:
10.1016/j.mimet.2014.05.018
PubMed:
24912108
PubMed Central:
PMC4166437

7. Taxonomic database and cut-off value for processing mcrA gene 454 pyrosequencing data by MOTHUR

Author:
Sizhong Yang; Susanne Liebner; Mashal Alawi; Oliver Ebenhöh; Dirk Wagner
Source:
Journal of microbiological methods 2014 v.103 pp. 3-5
ISSN:
0167-7012
Subject:
computer software; databases; genes; methanogens; phylogeny; ribosomal RNA; sequence analysis; species diversity
Abstract:
... The functional mcrA gene of methanogens can generate phylogeny as congruent as the 16S rRNA gene phylogeny. For the mcrA sequences amplified by mlas/mcrA-rev primers, we created a database for taxonomical classification and propose cut-off values for OTU clustering and further analysis of α- and β-diversity with the MOTHUR software. ...
DOI:
10.1016/j.mimet.2014.05.006

8. An automated method for the quantification of moving predators such as rotifers in biofilms by image analysis

Author:
T. Saur; K. Milferstedt; N. Bernet; R. Escudié
Source:
Journal of microbiological methods 2014 v.103 pp. 40-43
ISSN:
0167-7012
Subject:
Ciliophora; Nematoda; Protozoa; Rotifera; automation; biofilm; community structure; grazing; image analysis; microbial communities; microorganisms; population density; predators
Abstract:
... In natural environments as well as in industrial processes, microorganisms form biofilms. Eukaryotic microorganisms, like metazoans and protozoans, can shape the microbial communities because of their grazing activity. However, their influence on biofilm structure is often neglected because of the lack of appropriate methods to quantify their presence. In the present work, a method has been develo ...
DOI:
10.1016/j.mimet.2014.05.009

9. A critical combination of operating parameters can significantly increase the electrotransformation efficiency of a gram-positive Dietzia strain

Author:
Shelian Lu; Yong Nie; Yue-Qin Tang; Guangming Xiong; Xiao-Lei Wu
Source:
Journal of microbiological methods 2014 v.103 pp. 144-151
ISSN:
0167-7012
Subject:
Dietzia; bacteria; benzylpenicillin; electroporation; genetic engineering; hydrazides; industry; plasmids; polysorbates; protocols
Abstract:
... Dietzia spp. have broad potential applications in industries. However, genetic manipulation of these species is obstructed by their low transformation efficiency, which is in the range of 10⁴colony-forming units (CFU)μg⁻¹ exogenous DNA. In this study, both single-factor and orthogonal experiments were conducted to test the effects of competent cell concentration (parameter A), electroporation time ...
DOI:
10.1016/j.mimet.2014.05.015

10. An improved and versatile methodology to quantify biofilms formed on solid surfaces and exposed to the air–liquid interphase

Author:
S. Paytubi; P. Guirado; C. Balsalobre; C. Madrid
Source:
Journal of microbiological methods 2014 v.103 pp. 77-79
ISSN:
0167-7012
Subject:
biofilm; interphase; methodology
Abstract:
... To study pellicle formation, a new method has been developed to quantify biofilm formed on solid surfaces and exposed to air–liquid interphase. It is a versatile system since different adherent material surfaces might be tested. The methodology is a robust and reproducible approach to quantify biofilm. ...
DOI:
10.1016/j.mimet.2014.05.020

11. A new method for rapid screening of ester-producing yeasts using in situ HS-SPME

Author:
Juliano Garavaglia; Andressa Habekost; Thiago Rodrigues Bjerk; Rosana de Cassia de Souza Schneider; Juliane Elisa Welke; Cláudia Alcaraz Zini; Patricia Valente
Source:
Journal of microbiological methods 2014 v.103 pp. 1-2
ISSN:
0167-7012
Subject:
esters; headspace analysis; screening; solid phase microextraction; yeasts
Abstract:
... The selection of ester-producing yeasts is difficult because these molecules evaporate quickly, are extremely unstable and may be missed during analytical manipulation. We propose an easy, fast and efficient headspace-SPME method for screening of ester-producing yeasts directly at the extraction vials (in situ HS-SPME). ...
DOI:
10.1016/j.mimet.2014.05.001

12. A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches

Author:
Eoin Gunnigle; Jean-Baptiste Ramond; Aline Frossard; Mary Seeley; Don Cowan
Source:
Journal of microbiological methods 2014 v.103 pp. 118-123
ISSN:
0167-7012
Subject:
DNA; RNA; biopolymers; microbial communities; protocols; soil
Abstract:
... A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction. ...
DOI:
10.1016/j.mimet.2014.06.004

13. Artificial sweat composition to grow and sustain a mixed human axillary microbiome

Author:
Chris Callewaert; Benjamin Buysschaert; Els Vossen; Veerle Fievez; Tom Van de Wiele; Nico Boon
Source:
Journal of microbiological methods 2014 v.103 pp. 6-8
ISSN:
0167-7012
Subject:
humans; microbiome; microorganisms; odors; sweat; volatile organic compounds
Abstract:
... A novel artificial sweat composition, Skin Community Interaction simulation, designed to mimic the human axillary sweat, was compared to other artificial sweat compositions. Axillary microbiota grown in the novel composition closely resembled the original community. Volatile organic compound analysis showed good correlations with in vivo axillary (mal)odor components. ...
DOI:
10.1016/j.mimet.2014.05.005

14. Comparison of procedures for the extraction of supernatants and cytotoxicity tests in Vero cells, applied to assess the toxigenic potential of Bacillus spp. and Lactobacillus spp., intended for use as probiotic strains

Author:
Anicet R. Blanch; Javier Méndez; Susana Castel; Manuel Reina
Source:
Journal of microbiological methods 2014 v.103 pp. 64-69
ISSN:
0167-7012
Subject:
Bacillus cereus; Bacillus toyonensis; Lactobacillus; ammonium sulfate; animal nutrition; cytotoxicity; feeds; humans; methanol; probiotics; protein synthesis; protocols; ultrafiltration
Abstract:
... Interest in using Bacillus strains as probiotic components of animal feeds has grown in recent years. However, some of these strains, especially those taxonomically related to the Bacillus cereus group, may have enterotoxigenic activity. Assessment of their toxigenic potential by well-established and robust protocols is required before authorizing their use in animal nutrition. Three methods of ex ...
DOI:
10.1016/j.mimet.2014.05.019

15. Detection of stealthy small amphiphilic biomarkers

Author:
Rama Murthy Sakamuri; Petr Capek; Tobin J. Dickerson; Clifton E. Barry; Harshini Mukundan; Basil I. Swanson
Source:
Journal of microbiological methods 2014 v.103 pp. 112-117
ISSN:
0167-7012
Subject:
Mycobacterium leprae; Mycobacterium tuberculosis; biomarkers; blood serum; diagnostic techniques; infectious diseases; pathophysiology; proteins; therapeutics; vaccines
Abstract:
... Pathogen-specific biomarkers are secreted in the host during infection. Many important biomarkers are not proteins but rather small molecules that cannot be directly detected by conventional methods. However, these small molecule biomarkers, such as phenolic glycolipid-I (PGL-I) of Mycobacterium leprae and Mycobactin T (MbT) of Mycobacterium tuberculosis, are critical to the pathophysiology of inf ...
DOI:
10.1016/j.mimet.2014.05.012
PubMed:
24880131
PubMed Central:
PMC6402775

16. Development, optimization and validation of a rapid colorimetric microplate bioassay for neomycin sulfate in pharmaceutical drug products

Author:
Fabiane Lacerda Francisco; Alessandro Morais Saviano; Terezinha de Jesus Andreoli Pinto; Felipe Rebello Lourenço
Source:
Journal of microbiological methods 2014 v.103 pp. 104-111
ISSN:
0167-7012
Subject:
agar; antimicrobial properties; bioassays; chlorides; colorimetry; culture media; drug formulations; drugs; inoculum; neomycin; statistical analysis
Abstract:
... Microbiological assays have been used to evaluate antimicrobial activity since the discovery of the first antibiotics. Despite their limitations, microbiological assays are widely employed to determine antibiotic potency of pharmaceutical dosage forms, since they provide a measure of biological activity. The aim of this work is to develop, optimize and validate a rapid colorimetric microplate bioa ...
DOI:
10.1016/j.mimet.2014.05.023

17. Efficient transformation of Rhizopus delemar by electroporation of germinated spores

Author:
Sha Xu; Zhengxiong Zhou; Guocheng Du; Jingwen Zhou; Jian Chen
Source:
Journal of microbiological methods 2014 v.103 pp. 58-63
ISSN:
0167-7012
Subject:
DNA; Rhizopus; acetates; deoxycholic acid; dithiothreitol; electroporation; fungi; metabolic engineering; mycelium; spores
Abstract:
... High efficient transformation of mycelial fungi is essential to both metabolic engineering and physiological analysis of these industrially important microorganisms. However, transformation efficiencies for mycelial fungi are highly restricted by difficulties in colony formation and competent cell preparation. In this work, an innovative transformation procedure that could significantly improve th ...
DOI:
10.1016/j.mimet.2014.05.016

18. Enumeration of probiotic strains: Review of culture-dependent and alternative techniques to quantify viable bacteria

Author:
Catherine Davis
Source:
Journal of microbiological methods 2014 v.103 pp. 9-17
ISSN:
0167-7012
Subject:
RNA-directed DNA polymerase; bacteria; biochemical pathways; cell culture; flow cytometry; fluorescence in situ hybridization; gene amplification; image analysis; probiotics; propidium; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; viability
Abstract:
... Probiotics are live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. Standard culture techniques are commonly used to quantify probiotic strains, but cell culture only measures replicating cells. In response to the stresses of processing and formulation, some fraction of the live probiotic microbes may enter a viable but non-culturable state (VBNC) ...
DOI:
10.1016/j.mimet.2014.04.012

19. Identification and differentiation of food-related bacteria: A comparison of FTIR spectroscopy and MALDI-TOF mass spectrometry

Author:
Mareike Wenning; Franziska Breitenwieser; Regina Konrad; Ingrid Huber; Ulrich Busch; Siegfried Scherer
Source:
Journal of microbiological methods 2014 v.103 pp. 44-52
ISSN:
0167-7012
Subject:
Bacilli; Fourier transform infrared spectroscopy; bacteria; cost effectiveness; diagnostic techniques; food industry; matrix-assisted laser desorption-ionization mass spectrometry; protocols; ribosomal proteins; species identification
Abstract:
... The food industry requires easy, accurate, and cost-effective techniques for microbial identification to ensure safe products and identify microbial contaminations. In this work, FTIR spectroscopy and MALDI-TOF mass spectrometry were assessed for their suitability and applicability for routine microbial diagnostics of food-related microorganisms by analyzing their robustness according to changes i ...
DOI:
10.1016/j.mimet.2014.05.011

20. Identification of bacteria pathogenic to or associated with onion (Allium cepa) based on sequence differences in a portion of the conserved gyrase B gene

Author:
Jean M. Bonasera; Jo Ann E. Asselin; Steven V. Beer
Source:
Journal of microbiological methods 2014 v.103 pp. 138-143
ISSN:
0167-7012
Subject:
Allium cepa; Enterobacteriaceae; Gram-negative bacteria; bulbs; genes; leaves; onions; sequence diversity; soil
Abstract:
... We have developed a method for the identification of Gram-negative bacteria, particularly members of the Enterobacteriaceae, based on sequence variation in a portion of the gyrB gene. Thus, we identified, in most cases to species level, over 1000 isolates from onion bulbs and leaves and soil in which onions were grown. ...
DOI:
10.1016/j.mimet.2014.06.002
CHORUS:
10.1016/j.mimet.2014.06.002