Journal name: Journal of microbiological methods / Subject: Fusarium / Subject term: DNA

Author:: Sabrina Grube; Jutta Schönling; Alexander Prange
Source:: Journal of microbiological methods 2015 v.119 pp. 180-188
ISSN:: 0167-7012
Subject:: Alternaria; Claviceps purpurea; DNA; Fusarium; quantitative polymerase chain reaction
Abstract:: ... This article describes the development of a triplex real-time PCR system for the simultaneous detection of three major plant-pathogenic mold genera (Alternaria spp., Fusarium spp. and the species Claviceps purpurea). The designed genus-specific primer–probe systems were validated for sensitivity, specificity and amplification in the presence of background DNA. ...
DOI:: 10.1016/j.mimet.2015.10.017
: https://doi.org/10.1016/j.mimet.2015.10.017

Author:: Mariana G. Graça; Inneke M. van der Heijden; Lauro Perdigão; Cleison Taira; Silvia F. Costa; Anna S. Levin
Source:: Journal of microbiological methods 2016 v.123 pp. 39-43
ISSN:: 0167-7012
Subject:: DNA; Fusarium; detection limit; fungi; neutropenia; patients; polymerase chain reaction; water pollution
Abstract:: ... Fusarium is a waterborne fungus that causes severe infections especially in patients with prolonged neutropenia. Traditionally, the detection of Fusarium in water is done by culturing which is difficult and time consuming. A faster method is necessary to prevent exposure of susceptible patients to contaminated water. The objective of this study was to develop a molecular technique for direct detec ...
DOI:: 10.1016/j.mimet.2016.01.015
: https://doi.org/10.1016/j.mimet.2016.01.015

Author:: Katarzyna Pieczul; Agnieszka Perek; Krzysztof Kubiak
Source:: Journal of microbiological methods 2018 v.154 pp. 141-146
ISSN:: 0167-7012
Subject:: Alternaria; Cladosporium; DNA; Fusarium; Helminthosporium; Penicillium; Tilletia controversa; Tilletia laevis; Tilletia tritici; detection limit; fungi; protocols; rapid methods; smut diseases; teliospores; wheat
Abstract:: ... The study describes a novel diagnostic protocol based on a loop-mediated isothermal DNA amplification (LAMP) for identification of wheat grains infection by Tilletia laevis, Tilletia caries (common bunt) and Tilletia controversa (draft bunt). The presented data showed that the LAMP analysis is a simple, specific and rapid method that could be used for detection of Tilletia spp. in contaminated gra ...
DOI:: 10.1016/j.mimet.2018.10.018
: https://doi.org/10.1016/j.mimet.2018.10.018

Author:: Song Shan Wang; Hua Cui; Meng Ze Chen; Li Li; Yu Wu; Song Xue Wang
Source:: Journal of microbiological methods 2021 v.190 pp. 106319
ISSN:: 0167-7012
Subject:: 15-acetyldeoxynivalenol; 3-acetyldeoxynivalenol; DNA; Fusarium; Fusarium head blight; chemotypes; deoxynivalenol; disease control; droplets; geographical distribution; magnetism; metabolites; wheat; China; Yangtze River
Abstract:: ... Pathogens within Fusarium species are the primary agents of Fusarium head blight (FHB) of wheat, which bring about yield reduction and deoxynivalenol (DON) contamination and are of great concern worldwide. DON-producing Fusarium species can be classified into 3-acetyldeoxynivalenol (3ADON) and 15-acetyldeoxynivalenol (15ADON) chemotypes according to the trichothecene metabolites they produce. The ...
DOI:: 10.1016/j.mimet.2021.106319
: https://doi.org/10.1016/j.mimet.2021.106319

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