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You searched for: Journal Journal of microbiological methods Remove constraint Journal: Journal of microbiological methods Subject Alternaria Remove constraint Subject: Alternaria Subject DNA Remove constraint Subject: DNA
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1. Detection of Tilletia caries, Tilletia laevis and Tilletia controversa wheat grain contamination using loop-mediated isothermal DNA amplification (LAMP)

Author:
Pieczul, Katarzyna; Perek, Agnieszka; Kubiak, Krzysztof
Source:
Journal of microbiological methods 2018 v.154 pp. 141-146
ISSN:
0167-7012
Subject:
Alternaria; Cladosporium; DNA; Fusarium; Helminthosporium; Penicillium; Tilletia controversa; Tilletia laevis; Tilletia tritici; detection limit; fungi; protocols; rapid methods; smut diseases; teliospores; wheat
Abstract:
... The study describes a novel diagnostic protocol based on a loop-mediated isothermal DNA amplification (LAMP) for identification of wheat grains infection by Tilletia laevis, Tilletia caries (common bunt) and Tilletia controversa (draft bunt). The presented data showed that the LAMP analysis is a simple, specific and rapid method that could be used for detection of Tilletia spp. in contaminated gra ...
DOI:
10.1016/j.mimet.2018.10.018

2. Evaluation of a triplex real-time PCR system to detect the plant-pathogenic molds Alternaria spp., Fusarium spp. and C. purpurea

Author:
Grube, Sabrina; Schönling, Jutta; Prange, Alexander
Source:
Journal of microbiological methods 2015 v.119 pp. 180-188
ISSN:
0167-7012
Subject:
Alternaria; Claviceps purpurea; DNA; Fusarium; quantitative polymerase chain reaction
Abstract:
... This article describes the development of a triplex real-time PCR system for the simultaneous detection of three major plant-pathogenic mold genera (Alternaria spp., Fusarium spp. and the species Claviceps purpurea). The designed genus-specific primer–probe systems were validated for sensitivity, specificity and amplification in the presence of background DNA. ...
DOI:
10.1016/j.mimet.2015.10.017

3. A polyphasic approach for the characterization of endophytic Alternaria strains isolated from grapevines

Author:
Polizzotto, Rachele; Andersen, Birgitte; Martini, Marta; Grisan, Simone; Assante, Gemma; Musetti, Rita
Source:
Journal of microbiological methods 2012 v.88 no.1 pp. 162-171
ISSN:
0167-7012
Subject:
Alternaria; DNA; Vitis; chemotaxonomy; cluster analysis; endophytes; fungi; high performance liquid chromatography; metabolites; random amplified polymorphic DNA technique; sporulation
Abstract:
... A polyphasic approach was set up and applied to characterize 20 fungal endophytes belonging to the genus Alternaria, recovered from grapevine in different Italian regions. Morphological, microscopical, molecular and chemical investigations were performed and the obtained results were combined in a pooled cluster analysis. Following morphological analyses, all strains were grouped according to thei ...
DOI:
10.1016/j.mimet.2011.11.009