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- Author:
- Hildonen, Mathis; Kodama, Miyako; Puetz, Lara C.; Gilbert, M. Thomas P.; Limborg, Morten T.
- Source:
- Journal of microbiological methods 2019 v.160 pp. 42-48
- ISSN:
- 0167-7012
- Subject:
- DNA; Oncorhynchus mykiss; digestive system; dry ice; ethanol; fish; freezing; genomic libraries; intestinal microorganisms; labor; mammals; microbiome; new species; research programs; ribosomal RNA; sequence analysis; storage temperature; water content
- Abstract:
- ... Immediate freezing is perhaps the most preferred method used for preserving gut microbial samples, but research on sample preservation has been principally based around samples from mammalian species, and little is known about the advantages or disadvantages relating to different storage methods for fish guts. Fish gut samples may pose additional challenges due to the different chemical and enzyma ...
- DOI:
- 10.1016/j.mimet.2019.03.010
-
https://dx.doi.org/10.1016/j.mimet.2019.03.010
- Author:
- Nahid, Md A.; Campbell, Carmen E.; Fong, Keith S.K.; Barnhill, Jason C.; Washington, Michael A.
- Source:
- Journal of microbiological methods 2020 v.169 pp. 105833
- ISSN:
- 0167-7012
- Subject:
- Enterococcus; Escherichia coli; Pseudomonas aeruginosa; bacteria; biosensors; capacitance; cell culture; cell structures; digestive system; dose response; epithelial cells; human cell lines; interleukin-13; interleukin-1beta; interleukin-8; methicillin-resistant Staphylococcus aureus; microelectrodes; virulence
- Abstract:
- ... Virulence is the relative capacity of a pathogenic microorganism to cause damage in susceptible host cells such as those found in airway passages and the gut. In this study, the effect of clinical bacterial isolates on the monolayer integrity of cultured human alveolar basal epithelial cells (A549) was evaluated using the Electric Cell-Substrate Impedance Sensing (ECIS) system. ECIS is a morpholog ...
- DOI:
- 10.1016/j.mimet.2020.105833
-
https://dx.doi.org/10.1016/j.mimet.2020.105833
- Author:
- Peng, Xin; Yu, Ke-Qiang; Deng, Guan-Hua; Jiang, Yun-Xia; Wang, Yu; Zhang, Guo-Xia; Zhou, Hong-Wei
- Source:
- Journal of microbiological methods 2013 v.95 no.3 pp. 455-462
- ISSN:
- 0167-7012
- Subject:
- DNA; bacterial communities; boiling; cost effectiveness; digestive system; environmental health; feces; high-throughput nucleotide sequencing; human health; metagenomics; microbiome; microorganisms; ribosomal RNA; species diversity
- Abstract:
- ... Low cost and high throughput capacity are major advantages of using next generation sequencing (NGS) techniques to determine metagenomic 16S rRNA tag sequences. These methods have significantly changed our view of microorganisms in the fields of human health and environmental science. However, DNA extraction using commercial kits has shortcomings of high cost and time constraint. In the present st ...
- DOI:
- 10.1016/j.mimet.2013.07.015
-
http://dx.doi.org/10.1016/j.mimet.2013.07.015
- Author:
- O'Donnell, Michelle M.; Rea, Mary C.; O'Sullivan, Órla; Flynn, Cal; Jones, Beth; McQuaid, Albert; Shanahan, Fergus; Ross, R. Paul
- Source:
- Journal of microbiological methods 2016 v.129 pp. 109-116
- ISSN:
- 0167-7012
- Subject:
- biomass; cell viability; centrifugation; digestive system; feces; fermentation; freezing; glycerol; humans; inoculum; intestinal microorganisms; microbial communities; models; pelleting; phosphates; quantitative polymerase chain reaction; slurries
- Abstract:
- ... In-vitro gut fermentation systems provide suitable models for studying gut microbiota composition and functionality. However, such methods depend on the availability of donors and the assumption of reproducibility between microbial communities before experimental treatments commence. The aim of this study was to develop a frozen standardised inoculum (FSI) which minimizes inter-individual variatio ...
- DOI:
- 10.1016/j.mimet.2016.08.002
-
http://dx.doi.org/10.1016/j.mimet.2016.08.002
- Author:
- Takahashi, Keita; Hanamura, Yuki; Tokunoh, Nagisa; Kassai, Kohei; Matsunishi, Masaru; Watanabe, Shiori; Sugiyama, Tsuyoshi; Inoue, Naoki
- Source:
- Journal of microbiological methods 2019 v.159 pp. 62-68
- ISSN:
- 0167-7012
- Subject:
- Citrobacter rodentium; animal models; antibodies; bacteria; blood serum; body weight changes; death; digestive system; edible vaccines; enterohemorrhagic Escherichia coli; enteropathogenic Escherichia coli; genes; hyperplasia; immunoglobulin G; mice; oral administration; oral vaccination; pathogens; protective effect; virulence
- Abstract:
- ... Evaluation of the efficacy of vaccine candidates that prevent enteropathogenic and enterohemorrhagic Escherichia coli (EPEC/EHEC) infection in mouse models is difficult due to their limited pathogenicity in mice. Citrobacter rodentium, a murine pathogenic bacterium that shares its infection strategy and virulence genes with EPEC/EHEC, has been used as a model pathogen to develop novel vaccine stra ...
- DOI:
- 10.1016/j.mimet.2019.02.016
-
https://dx.doi.org/10.1016/j.mimet.2019.02.016
- Author:
- Yang, Kundi; Xu, Mengyang; Zhong, Fanyi; Zhu, Jiangjiang
- Source:
- Journal of microbiological methods 2018 v.154 pp. 147-155
- ISSN:
- 0167-7012
- Subject:
- Lactobacillus; data quality; digestive system; functional properties; humans; lactic acid; lactic acid bacteria; metabolites; metabolomics; monitoring; probiotics; spectrometers; sugars
- Abstract:
- ... Lactobacillus, the major genus of lactic acid bacteria group, plays functional roles in the human body, for example, convert sugars to lactic acid. They are the significant microbiota which can be found at a number of human body sites, such as the digestive system, urinary system, and genital system. A number of Lactobacillus species are often used as probiotics and can benefit host health when ad ...
- DOI:
- 10.1016/j.mimet.2018.10.013
-
https://dx.doi.org/10.1016/j.mimet.2018.10.013
- Author:
- McDonald, Julie A.K.; Fuentes, Susana; Schroeter, Kathleen; Heikamp-deJong, Ineke; Khursigara, Cezar M.; de Vos, Willem M.; Allen-Vercoe, Emma
- Source:
- Journal of microbiological methods 2015 v.108 pp. 36-44
- ISSN:
- 0167-7012
- Subject:
- biofilm; digestive system; humans; in vivo studies; intestinal microorganisms; models
- Abstract:
- ... In vivo studies of human mucosal gut microbiota are often limited to end-point analyses and confounded by bowel cleansing procedures. Therefore, we used biofilm reactors to incorporate a simulated mucosal environment into an in vitro gut chemostat model. Communities developed were complex, reproducible, distinct, and representative of in vivo communities. ...
- DOI:
- 10.1016/j.mimet.2014.11.007
-
http://dx.doi.org/10.1016/j.mimet.2014.11.007
