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- Marisol Aguirre; Anat Eck; Marjorie E. Koenen; Paul H.M. Savelkoul; Andries E. Budding; Koen Venema
- Journal of microbiological methods 2015 v.117 pp. 78-84
- Actinobacteria; Bacteroidetes; Firmicutes; Fusobacteria; Proteobacteria; Verrucomicrobia; acetates; bacteria; feces; fermentation; freezing; glycerol; humans; inoculum; propionic acid; short chain fatty acids
- ... This study investigated the optimal preservation approach to prepare human feces as inoculum for in vitro fermentations as an alternative to the use of fresh feces. The four treatments studied were: Treatment 1) fresh feces resuspended in dialysate solution+glycerol; Treatment 2) fresh feces resuspended in dialysate solution+glycerol and then stored at −80°C; Treatment 3) fecal sample frozen with ...
- Andrée F. Maheux; Stéphanie Brodeur; Ève Bérubé; Dominique K. Boudreau; Jehane Y. Abed; Maurice Boissinot; Luc Bissonnette; Michel G. Bergeron
- Journal of microbiological methods 2018 v.154 pp. 134-140
- Escherichia albertii; bacteria; feces; fermentation; isolation techniques; lactose; pathogenicity; phenotype; polymerase chain reaction; species identification
- ... Initially, Escherichia albertii has been described as a non-lactose fermenting bacterium and methods used to isolate it were first based on this phenotypic property. However, a recent study showed a variable lactose fermentation phenotype for E. albertii suggesting that this microorganism could have been underestimated by previous studies using isolation methods based on lactose fermentation. In t ...