U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Journal name Journal of microbiological methods Remove constraint Journal name: Journal of microbiological methods Subject term isolation techniques Remove constraint Subject term: isolation techniques
Start Over

Search

20  Search Results

1 - 20 of 20
Number of results to display per page

Search Results

1. Establishment of a semi-automated pathogen DNA isolation from whole blood and comparison with commercially available kits

Author:
Herbert Wiesinger-Mayr; Elena Jordana-Lluch; Elisa Martró; Silvia Schoenthaler; Christa Noehammer
Source:
Journal of microbiological methods 2011 v.85 no.3 pp. 206-213
ISSN:
0167-7012
Subject:
DNA; bacteria; blood; centrifugation; detection limit; genes; humans; isolation techniques; microarray technology; nucleic acid hybridization; pathogen identification; pathogens; polymerase chain reaction; ribosomal RNA
Abstract:
... Molecular methods for bacterial pathogen identification are gaining increased importance in routine clinical diagnostic laboratories. Achieving reliable results using DNA based technologies is strongly dependent on pre-analytical processes including isolation of target cells and their DNA of high quality and purity. In this study a fast and semi-automated method was established for bacterial DNA i ...
DOI:
10.1016/j.mimet.2011.03.003

2. High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples

Author:
Jeff A. Brady; Jennifer B. Faske; Jessica M. Castañeda-Gill; Jonathan L. King; Forrest L. Mitchell
Source:
Journal of microbiological methods 2011 v.86 no.3 pp. 310-312
ISSN:
0167-7012
Subject:
DNA; Xylella fastidiosa; insects; isolation techniques; microbial detection; plants (botany); polymerase chain reaction
Abstract:
... We report an inexpensive, high-throughput method for isolating DNA from insect and plant samples for the purpose of detecting Xylella fastidiosa infection. Existing methods often copurify inhibitors of DNA polymerases, limiting their usefulness for PCR-based detection assays. When compared to commercially available kits, the method provides enhanced pathogen detection at a fraction of the cost. ...
DOI:
10.1016/j.mimet.2011.06.007

3. Isolation and identification of quorum quenching bacteria from environmental samples

Author:
Steven E.A. Christiaen; Gilles Brackman; Hans J. Nelis; Tom Coenye
Source:
Journal of microbiological methods 2011 v.87 no.2 pp. 213-219
ISSN:
0167-7012
Subject:
Pseudomonas aeruginosa; bacteria; biosensors; carbon; cell communication; communications technology; gene expression; genes; heat stability; heat treatment; infectious diseases; isolation techniques; lactones; molecular weight; nitrogen; pathogens; peptidase K; quorum sensing; ribosomal RNA; virulence
Abstract:
... A large number of Gram-negative pathogens produce N-acylhomoserine lactones (AHLs) as signal molecules for quorum sensing (QS). This cell–cell communication system allows them to coordinate gene expression and regulate virulence. Therefore, strategies to inhibit QS are promising for the control of infectious diseases. The aim of the present study was to develop a high-throughput method for the iso ...
DOI:
10.1016/j.mimet.2011.08.002

4. Optimizing Bacillus subtilis spore isolation and quantifying spore harvest purity

Author:
Zoë R. Harrold; Mikaela R. Hertel; Drew Gorman-Lewis
Source:
Journal of microbiological methods 2011 v.87 no.3 pp. 325-329
ISSN:
0167-7012
Subject:
Bacillus subtilis; biochemistry; biomass; computer software; endospores; fluorescence microscopy; image analysis; isolation techniques; quality control; vegetative cells
Abstract:
... Investigating the biochemistry, resilience and environmental interactions of bacterial endospores often requires a pure endospore biomass free of vegetative cells. Numerous endospore isolation methods, however, neglect to quantify the purity of the final endospore biomass. To ensure low vegetative cell contamination we developed a quality control technique that enables rapid quantification of endo ...
DOI:
10.1016/j.mimet.2011.09.014

5. An enhanced technique combining pre-enrichment and passive filtration increases the isolation efficiency of Campylobacter jejuni and Campylobacter coli from water and animal fecal samples

Author:
Cassandra C. Jokinen; Jacqueline M. Koot; Catherine D. Carrillo; Victor P.J. Gannon; Claire M. Jardine; Steven K. Mutschall; Edward Topp; Eduardo N. Taboada
Source:
Journal of microbiological methods 2012 v.91 no.3 pp. 506-513
ISSN:
0167-7012
Subject:
Campylobacter coli; Campylobacter jejuni; agar; animals; cefoperazone; charcoal; chicken meat; cost effectiveness; epidemiology; feces; filtration; genetic variation; isolation techniques; surface water
Abstract:
... Improved isolation techniques from environmental water and animal samples are vital to understanding Campylobacter epidemiology. In this study, the efficiency of selective enrichment in Bolton Broth (BB) followed by plating on charcoal cefoperazone deoxycholate agar (CCDA) (conventional method) was compared with an approach combining BB enrichment and passive filtration (membrane method) adapted f ...
DOI:
10.1016/j.mimet.2012.09.005

6. Novel 16S rRNA based PCR method targeting Deinococcus spp. and its application to assess the diversity of deinococcal populations in environmental samples

Author:
Ruchi Chaturvedi; G. Archana
Source:
Journal of microbiological methods 2012 v.90 no.3 pp. 197-205
ISSN:
0167-7012
Subject:
Deinococcus; denaturing gradient gel electrophoresis; ionization; isolation techniques; polymerase chain reaction; radiation resistance; rapid methods; ribosomal RNA; sequence analysis; soil sampling; India
Abstract:
... The members of the genus Deinococcus are extensively studied because of their exemplary radiation resistance. Both ionizing and non-ionizing rays are routinely employed to select upon the radiation resistant deinococcal population and isolate them from the majority of radiation sensitive population. There are no studies on the development of molecular tools for the rapid detection and identificati ...
DOI:
10.1016/j.mimet.2012.05.005

7. A robust and efficient method for the isolation of DNA-free, pure and intact RNA from Mycobacterium tuberculosis

Author:
Balaji Venkataraman; Nidhi Gupta; Amita Gupta
Source:
Journal of microbiological methods 2013 v.93 no.3 pp. 198-202
ISSN:
0167-7012
Subject:
Mycobacterium tuberculosis; isolation techniques; ribosomal RNA; toxic substances; transcriptome
Abstract:
... We describe a robust method for the isolation of pure, intact RNA suitable for transcriptome studies from mycobacteria with consistent yields of 1μg to 3μg total RNA per 10⁷ cells. The method reduces the use of hazardous chemicals and incorporates protocols for efficient removal of gDNA and rRNA. ...
DOI:
10.1016/j.mimet.2013.03.018

8. An anion-exchange method to concentrate dissolved DNA from aquifer water

Author:
Zhen Li; Brandon R. Briggs; Peter P. Sheridan; Malcolm S. Shields
Source:
Journal of microbiological methods 2013 v.93 no.1 pp. 1-8
ISSN:
0167-7012
Subject:
anion exchange; aquifers; genes; isolation techniques; plasmids; polymerase chain reaction; restriction fragment length polymorphism; ribosomal RNA; Snake River
Abstract:
... A rapid DNA isolation method was developed to concentrate dissolved DNA (dDNA) in aquifer water for molecular analysis. The aquifer dDNA from the Eastern Snake River Plain Aquifer (ESRPA) was extracted and concentrated using a new method with an anion-exchange Mustang® Q membrane. The concentration of aquifer dDNA in this study ranged from 60 to 264.5ngl⁻¹ in ESRPA aquifer wells. DNA stability in ...
DOI:
10.1016/j.mimet.2013.01.014

9. Improved protocol for isolation of Campylobacter spp. from retail broiler meat and use of pulsed field gel electrophoresis for the typing of isolates

Author:
Omar A. Oyarzabal; Aretha Williams; Ping Zhou; Mansour Samadpour
Source:
Journal of microbiological methods 2013 v.95 no.1 pp. 76-83
ISSN:
0167-7012
Subject:
denaturing gradient gel electrophoresis; Campylobacter coli; thighs; chicken meat; genome; polymerase chain reaction; peptones; aerobiosis; isolation techniques; breasts; pulsed-field gel electrophoresis; agar; anti-infective agents; population; Alabama
Abstract:
... To improve the detection of Campylobacter spp. in retail broiler meat, a reference method (R subsamples) based on the enrichment of 25g of meat in Bolton broth at 42°C under microaerobiosis was compared with an alternative method (A subsamples) consisting in the rinsing of meat samples for 30s in buffered peptone water with antimicrobials with incubation at 42°C under aerobiosis. One piece of meat ...
DOI:
10.1016/j.mimet.2013.03.008

10. Quantification of Cryptosporidium parvum in natural soil matrices and soil solutions using qPCR

Author:
Emre Koken; Christophe J.G. Darnault; Astrid R. Jacobson; David Powelson; William Hendrickson
Source:
Journal of microbiological methods 2013 v.92 no.2 pp. 135-144
ISSN:
0167-7012
Subject:
Cryptosporidium parvum; DNA; detection limit; isolation techniques; magnesium chloride; microscopy; oocysts; physicochemical properties; quantitative polymerase chain reaction; soil sampling; sucrose; temperature; thawing
Abstract:
... Traditional microscopy methods for the detection and quantification of Cryptosporidium parvum in soil matrices are time-consuming, labor-intensive, and lack sensitivity and specificity. This research focused on developing a qPCR protocol for the sensitive and specific detection and quantification of C. parvum in natural soil matrices and soil–water extracts. The physico-chemical parameters — lysis ...
DOI:
10.1016/j.mimet.2012.11.015

11. Protein complex purification from Thermoplasma acidophilum using a phage display library

Author:
Ágnes Hubert; Yasuo Mitani; Tomohiro Tamura; Marius Boicu; István Nagy
Source:
Journal of microbiological methods 2014 v.98 pp. 15-22
ISSN:
0167-7012
Subject:
Escherichia coli; antigens; bacteriophages; electron microscopy; gel chromatography; isolation techniques; proteasome endopeptidase complex; proteins
Abstract:
... We developed a novel protein complex isolation method using a single-chain variable fragment (scFv) based phage display library in a two-step purification procedure. We adapted the antibody-based phage display technology which has been developed for single target proteins to a protein mixture containing about 300 proteins, mostly subunits of Thermoplasma acidophilum complexes. T. acidophilum prote ...
DOI:
10.1016/j.mimet.2013.12.010

12. An infrared diagnostic system to detect causal agents of grapevine trunk diseases

Author:
Cecile Levasseur-Garcia; Hugo Malaurie; Nathalie Mailhac
Source:
Journal of microbiological methods 2016 v.131 pp. 1-6
ISSN:
0167-7012
Subject:
Vitis vinifera; financial economics; infrared spectroscopy; isolation techniques; microbial contamination; multivariate analysis; polymerase chain reaction; quality control; spectrometers; vineyards; viticulture; wine industry; France
Abstract:
... In most vineyards worldwide, agents of grapevine trunk diseases represent a real threat for viticulture and are responsible for significant economic loss to the wine industry. The conventional microbiological isolation technique used to diagnose this disease is tedious and frequently leads to false negatives. Thus, a dire need exists for an alternative method to detect this disease. One possible w ...
DOI:
10.1016/j.mimet.2016.09.022

13. Technical note: Discard the specimen collection swab directly at point of care to improve extensive automated processing in clinical microbiology laboratories

Author:
Manuela Avolio; Shamanta Grosso; Graziano Bruschetta; Alessandro Camporese
Source:
Journal of microbiological methods 2016 v.129 pp. 20-22
ISSN:
0167-7012
Subject:
bacteria; culture media; isolation techniques; pathogens; point-of-care systems
Abstract:
... We compared, in terms of microorganisms recovery, the discard of specimen collection swab, after swirling into its medium, directly at point of care, with its placing into the medium and vortexing on arrival in the laboratory. Our results show that these two procedures are overlapped in terms of bacterial recovery. ...
DOI:
10.1016/j.mimet.2016.07.024

14. Development of a rapid, one-step screening method for the isolation of presumptive proteolytic enterococci

Author:
Ken Graham; Rosemary Rea; Paul Simpson; Helena Stack
Source:
Journal of microbiological methods 2017 v.132 pp. 99-105
ISSN:
0167-7012
Subject:
Enterococcus; agar; dried skim milk; enzymes; feces; isolation techniques; kanamycin; lactic acid bacteria; probiotics; proteolysis; raw milk; screening; skim milk; soil; virulence
Abstract:
... Enterococci show higher proteolytic activities than other lactic acid bacteria and thus have received considerable attention in scientific literature in recent years. Proteolytic enzymes of enterococci have warranted the use of some species as starter, adjuncts or protective cultures and as probiotics, while in some strains they have also been linked with virulence. Consequently, the isolation and ...
DOI:
10.1016/j.mimet.2016.11.016

15. Immobilization of microalgae cells in alginate facilitates isolation of DNA and RNA

Author:
Blanca R. Lopez; Juan-Pablo Hernandez; Yoav Bashan; Luz E. de-Bashan
Source:
Journal of microbiological methods 2017 v.135 pp. 96-104
ISSN:
0167-7012
Subject:
Chlorella; immobilized cells; proteins; quantitative polymerase chain reaction; ribosomal RNA; cell walls; DNA; alginates; cetyltrimethylammonium bromide; glass; reverse transcriptase polymerase chain reaction; isolation techniques; reverse transcription; polymers; microalgae; metabolites
Abstract:
... Isolation of nucleic acids from Chlorella is difficult, given the chemically complex nature of their cell walls and variable production of metabolites. Immobilization of microalgae in polymers adds additional difficulty. Here, we modified, amended, and standardized methods for isolation of nucleic acids and compared the yield of DNA and RNA from free-living and encapsulated microalgae C. sorokinia ...
DOI:
10.1016/j.mimet.2017.02.005

16. Method for isolation of both lactose-fermenting and – non-fermenting Escherichia albertii strains from stool samples

Author:
Andrée F. Maheux; Stéphanie Brodeur; Ève Bérubé; Dominique K. Boudreau; Jehane Y. Abed; Maurice Boissinot; Luc Bissonnette; Michel G. Bergeron
Source:
Journal of microbiological methods 2018 v.154 pp. 134-140
ISSN:
0167-7012
Subject:
Escherichia albertii; bacteria; feces; fermentation; isolation techniques; lactose; pathogenicity; phenotype; polymerase chain reaction; species identification
Abstract:
... Initially, Escherichia albertii has been described as a non-lactose fermenting bacterium and methods used to isolate it were first based on this phenotypic property. However, a recent study showed a variable lactose fermentation phenotype for E. albertii suggesting that this microorganism could have been underestimated by previous studies using isolation methods based on lactose fermentation. In t ...
DOI:
10.1016/j.mimet.2018.09.008

17. A simple method for direct isolation of N-acyl-L-homoserine lactone mediated biofilm-forming rhizobacteria from roots

Author:
Jamal Farheen Begum; Murugesh Tamilarasi; Periaswamy Pushpakanth; Dananjeyan Balachandar
Source:
Journal of microbiological methods 2019 v.156 pp. 34-39
ISSN:
0167-7012
Subject:
Aeromonas hydrophila; Aeromonas punctata; Chromobacterium violaceum; Pseudomonas; bacterial growth; biofilm; biosensors; culture media; isolation techniques; nucleotide sequences; phylogeny; quorum sensing; rhizosphere; rhizosphere bacteria; ribosomal RNA; rice; roots; violacein
Abstract:
... Plant-associated bacteria produce quorum sensing (QS) signals for community (biofilm) formation and functioning in the rhizosphere. The QS-positive biofilm-forming rhizobacteria that excel benefits to the plants are now gaining increased importance for agricultural use due to their high competitiveness. However, there is no method available to distinguish these bacteria from the roots of a plant t ...
DOI:
10.1016/j.mimet.2018.11.018

18. Evaluation of filter paper as a means to transport inactivated bacteria for identification using the MALDI-TOF MS system

Author:
M.S. Carneiro; A. Fracasso; O.V.A. Lovison; F. Barreto; A.L. Barth
Source:
Journal of microbiological methods 2020 v.171 pp. 105863
ISSN:
0167-7012
Subject:
bacteria; filters; isolation techniques; matrix-assisted laser desorption-ionization mass spectrometry
Abstract:
... A novel approach to transport inactivated bacteria in filter paper for identification in the MALDI-TOF MS was evaluated. Seventy four bacterial isolates were evaluated and the approach presented sensitivity of 97.3% and specificity of 100%. Inactivated bacteria in filter paper are a safer alternative to transport bacteria for MALDI-TOF MS identification. ...
DOI:
10.1016/j.mimet.2020.105863

19. Isolation of third generation cephalosporin resistant Enterobacteriaceae from retail meats and detection of extended spectrum beta-lactamase activity

Author:
Mary Rao; Anna Laidlaw; Leo Li; Kristian Young; Sandeep Tamber
Source:
Journal of microbiological methods 2021 v.189 pp. 106314
ISSN:
0167-7012
Subject:
Enterobacteriaceae; agar; antibiotic resistance; beta-lactamase; cefoxitin; ceftriaxone; chickens; culture media; isolation techniques; microorganisms; pork; sulfates; taxonomy; veal
Abstract:
... Various methods have been described to isolate third generation cephalosporin (3GC) resistant Enterobacteriaceae from foods, but it is not known how comparable they are between studies. Here, the performance of five enrichment broths and two selective agars are compared for their ability to isolate 3GC resistant Enterobacteriaceae from retail chicken, beef, pork, and veal samples. The results show ...
DOI:
10.1016/j.mimet.2021.106314

20. Make microalgal cultures axenic again – a fast and simple workflow utilizing fluorescence-activated cell sorting

Author:
Philipp Doppler; Ricarda Kriechbaum; Benedikt Singer; Oliver Spadiut
Source:
Journal of microbiological methods 2021 v.186 pp. 106256
ISSN:
0167-7012
Subject:
Chlorophyta; Cyanobacteria; algae culture; axenic culture; flow cytometry; isolation techniques; journals
Abstract:
... Since the removal of contaminations in microalgal cultures is extremely laborious and time-consuming, we developed a rapid workflow to obtain axenicity by a combination of fluorescence-activated cell sorting (FACS) and plate spreading. During method development, several cyanobacteria and green algae strains were successfully made axenic. At the end, method transferability to another FACS device wa ...
DOI:
10.1016/j.mimet.2021.106256