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You searched for: Journal name Journal of molecular catalysis Remove constraint Journal name: Journal of molecular catalysis Publication year rev 7984-2016 Remove constraint Publication year rev: 7984-2016 Source 2016 v.124 Remove constraint Source: 2016 v.124
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1. In situ immobilization of Candida antarctica B lipase in polyurethane foam support

Author:
Nadia Ligianara D. Nyari; Ilizandra A. Fernandes; Cindy E. Bustamante-Vargas; Clarisse Steffens; Débora de Oliveira; Jamile Zeni; Elisandra Rigo; Rogério M. Dallago
Source:
Journal of molecular catalysis 2016 v.124 pp. 52-61
ISSN:
1381-1177
Subject:
Pseudozyma antarctica; carboxylic ester hydrolases; catalysts; catalytic activity; enzyme activity; enzyme stability; foams; food industry; oleic acid; polyols; polyurethanes; propionates; refrigeration; temperature
Abstract:
... Candida antarctica B (CALB) lipase was immobilized using polyurethane (PU) foam as support by confinement method. This can be a promising technique due to the low cost of the support, simple procedure of immobilization and the possibility of using the derivative as catalyst for the food industry reactions. CALB immobilization on PU was performed using 6mL polyol and 4mL isocyanate (60–40%, v/v), w ...
DOI:
10.1016/j.molcatb.2015.12.003

2. Two-step enzymatic synthesis of tyramine from raw pyruvate fermentation broth

Author:
Hongjuan Zhang; Yang Lu; Siping Wu; Yu Wei; Qian Liu; Junzhong Liu; Qingcai Jiao
Source:
Journal of molecular catalysis 2016 v.124 pp. 38-44
ISSN:
1381-1177
Subject:
catalytic activity; culture media; drugs; immobilized cells; metabolites; pyruvic acid; tyramine; tyrosine; tyrosine decarboxylase
Abstract:
... Tyramine, as a metabolite of tyrosine, is an important intermediate in synthesizing some drugs and medicinal materials. In this study, an efficient method for producing tyramine was developed by a two-step biocatalytic reaction with recombinant tyrosine phenol-lyase whole cells and tyrosine decarboxylase immobilized cells. Raw pyruvate fermentation broth was used as substrate of tyrosine phenol-ly ...
DOI:
10.1016/j.molcatb.2015.11.024

3. A combined temperature-pH study of urease kinetics. Assigning pKa values to ionizable groups of the active site involved in the catalytic reaction

Author:
Barbara Krajewska
Source:
Journal of molecular catalysis 2016 v.124 pp. 70-76
ISSN:
1381-1177
Subject:
Klebsiella aerogenes; active sites; buffers; catalytic activity; enthalpy; histidine; pH; temperature; urease
Abstract:
... A combined temperature-pH study of urease kinetics was performed with the primary objective to assign the observed pKa values to the ionizable groups of the enzyme active site involved in the reaction. This was done in view of the fact that the identity of the groups detected by pH studies reported in the literature has never been conclusively resolved. Accordingly, herein the urease kinetic param ...
DOI:
10.1016/j.molcatb.2015.11.021

4. Asymmetric Morita–Baylis–Hillman reaction catalyzed by pepsin

Author:
Jing-Wen Xue; Jian Song; Ian C.K. Manion; Yan-Hong He; Zhi Guan
Source:
Journal of molecular catalysis 2016 v.124 pp. 62-69
ISSN:
1381-1177
Subject:
aldehydes; catalytic activity; cyclohexanes; octane; pepsin; phosphates; swine
Abstract:
... Pepsin from porcine gastric mucous shown catalytic promiscuity was first discovered to catalyze the Morita–Baylis–Hillman (MBH) reaction between aromatic aldehydes with 2-cyclohexen-1-one or 2-cyclopenten-1-one in a two-phase medium of phosphate buffer/cyclohexane in the presence of 1,4-diazabicyclo[2.2.2]octane (DABCO). The best results of the corresponding MBH products up to 77% yield and 38% ee ...
DOI:
10.1016/j.molcatb.2015.12.002

5. Cheap and environmentally sustainable stereoselective arylketones reduction by Lactobacillus reuteri whole cells

Author:
F.M. Perna; M.A. Ricci; A. Scilimati; M.C. Mena; I. Pisano; L. Palmieri; G. Agrimi; P. Vitale
Source:
Journal of molecular catalysis 2016 v.124 pp. 29-37
ISSN:
1381-1177
Subject:
Lactobacillus reuteri; biotransformation; catalysts; catalytic activity; cheeses; glucose; hydrolysates; lactose; lignocellulose; stereochemistry; substrate specificity; temperature
Abstract:
... Various Lactobacillus reuteri strains were investigated as whole cell catalysts for the bioreduction of acetophenone into optically active (R)-1-phenylethanol. L. reuteri DSM 20016 strain gave an almost complete substrate conversion, in a short reaction time and enantiomeric excess up to 99%. The acetophenone bioreduction was used as a model reaction to optimize temperature and reducing equivalent ...
DOI:
10.1016/j.molcatb.2015.11.025

6. Cloning with kinetic and thermodynamic insight of a novel hyperthermostable β-glucosidase from Thermotoga naphthophila RKU-10T with excellent glucose tolerance

Author:
Fatima Akram; Ikram ul Haq; Mahmood Ali Khan; Zahid Hussain; Hamid Mukhtar; Kaleem Iqbal
Source:
Journal of molecular catalysis 2016 v.124 pp. 92-104
ISSN:
1381-1177
Subject:
Escherichia coli; Thermotoga; amino acids; bacteria; beta-glucosidase; biomass; catalytic activity; cations; cellobiose; denaturation; detergents; enzyme activity; gene overexpression; genes; glucose; glucose tolerance; glycosides; half life; heat tolerance; heat treatment; hydrolysis; hydrophobic interaction chromatography; industrial applications; ion exchange; molecular weight; pH; renewable resources; temperature; thermal stability
Abstract:
... With a paradigm shift in industry, moving from natural fuels to alternative renewable resource utilization, the need of efficient thermostable cellulases are expected to increase in future. β-glucosidase, an essential member of cellulases that plays a critical role in cellulosic biomass degradation and in many biological processes. Therefore, a novel β-glucosidase gene encodes a protein (BglA) of ...
DOI:
10.1016/j.molcatb.2015.12.005

7. One-pot enzymatic reaction sequence for the syntheses of d-glyceraldehyde 3-phosphate and l-glycerol 3-phosphate

Author:
Getachew S. Molla; Roland Wohlgemuth; Andreas Liese
Source:
Journal of molecular catalysis 2016 v.124 pp. 77-82
ISSN:
1381-1177
Subject:
Candida boidinii; catalytic activity; enzymatic reactions; formate dehydrogenase; fructose-bisphosphate aldolase; glyceraldehyde 3-phosphate; glycerol-3-phosphate dehydrogenase (NAD); glycerophosphates; model validation; models; muscles; rabbits; reaction kinetics; thermodynamics
Abstract:
... A one-pot enzymatic reaction sequence for the synthesis of optically pure d-glyceraldehyde 3-phosphate (d-GAP) and l-glycerol 3-phosphate (sn-G3P) was designed using fructose-bisphosphate aldolase from rabbit muscle (RAMA), sn-glycerol 3-phosphate dehydrogenase (sn-G3PDH) and formate dehydrogenase from Candida boidinii (FDH). The reaction sequence significantly improves the aldol cleavage of d-fru ...
DOI:
10.1016/j.molcatb.2015.12.004

8. Partial purification and characterization of L-asparaginase from an endophytic Talaromyces pinophilus isolated from the rhizomes of Curcuma amada

Author:
Prajna Rao Krishnapura; Prasanna D. Belur
Source:
Journal of molecular catalysis 2016 v.124 pp. 83-91
ISSN:
1381-1177
Subject:
Curcuma amada; Talaromyces pinophilus; asparaginase; calcium; catalytic activity; copper; cysteine; endophytes; enzyme activity; fungi; glutaminase; glutamine; iron; magnesium; manganese; metal ions; pH; rhizomes; temperature; thiols; zinc
Abstract:
... l-Asparaginase is a commercially significant enzyme. There exists a demand for a broad variety of microbial l-asparaginases with characteristics compatible with its different applications. Endophytic microorganisms, in particular are emerging as potential sources of l-asparaginases.The current work involves partial purification and characterization of l-asparaginase from Talaromyces pinophilus, an ...
DOI:
10.1016/j.molcatb.2015.12.007

9. Purification and characterization of a new inducible thermostable extracellular lipolytic enzyme from the thermoacidophilic archaeon Sulfolobus solfataricus P1

Author:
Yun-Ho Choi; Young-Jun Park; Sung-Jin Yoon; Hee-Bong Lee
Source:
Journal of molecular catalysis 2016 v.124 pp. 11-19
ISSN:
1381-1177
Subject:
Sulfolobus solfataricus; active sites; aspartic acid; butanol; carboxylesterase; catalytic activity; corn oil; enzyme stability; gel chromatography; histidine; industrial applications; pH; palmitates; polyacrylamide gel electrophoresis; serine; sodium dodecyl sulfate; substrate specificity; thermal stability; thin layer chromatography; triolein; unsaturated fatty acids; urea
Abstract:
... A new thermostable extracellular lipolytic enzyme, induced from the thermoacidophilic archaeon Sulfolobus solfataricus P1 using corn oil as an inducer, was purified to apparent homogeneity by butanol extraction and two column chromatographies using DEAE-Sepharose followed by Butyl-Sepharose. The purified enzyme assessed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and ...
DOI:
10.1016/j.molcatb.2015.11.023

10. Stabilization of an amine transaminase for biocatalysis

Author:
Shan Chen; Henrik Land; Per Berglund; Maria Svedendahl Humble
Source:
Journal of molecular catalysis 2016 v.124 pp. 20-28
ISSN:
1381-1177
Subject:
Chromobacterium violaceum; additives; amines; biocatalysis; dimethyl sulfoxide; enzyme stability; glycerol; iso-octanes; melting point; methanol; solvents; sucrose; surfactants; temperature
Abstract:
... The amine transaminase from Chromobacterium violaceum (Cv-ATA) is a well-known enzyme to achieve chiral amines of high enantiomeric excess in laboratory scales. However, the low operational stability of Cv-ATA limits the enzyme applicability on larger scales. In order to improve the operational stability of Cv-ATA, and thereby extending its applicability, factors (additives, co-solvents, organic s ...
DOI:
10.1016/j.molcatb.2015.11.022

11. Synthetic analog of anticancer drug daunorubicin from daunorubicinone using one-pot enzymatic UDP-recycling glycosylation

Author:
Luan Luong Chu; Ramesh Prasad Pandey; Ju Yong Shin; Hye Jin Jung; Jae Kyung Sohng
Source:
Journal of molecular catalysis 2016 v.124 pp. 1-10
ISSN:
1381-1177
Subject:
antineoplastic activity; catalytic activity; doxorubicin; glucosides; glycosylation; neoplasms; pH; recycling; sugars; temperature; water solubility
Abstract:
... Doxorubicin and daunorubicin are two widely used anticancer drugs containing deoxyaminosugar unit in their structure. Altering the sugar moiety could generate novel drugs, which could have different biological potency than daunorubicin and doxorubicin. In this study, daunorubicinone was used as a substrate for the production of two different novel glucoside derivatives via enzymatic glycosylation, ...
DOI:
10.1016/j.molcatb.2015.11.020

12. Tryptophan-surface modification of versatile peroxidase from Bjerkandera adusta enhances its catalytic performance

Author:
Flor Sánchez-Alejandro; Karla Juarez-Moreno; Maria Camilla Baratto; Riccardo Basosi; Rafael Vazquez-Duhalt
Source:
Journal of molecular catalysis 2016 v.124 pp. 45-51
ISSN:
1381-1177
Subject:
Bjerkandera adusta; active sites; catalytic activity; electron transfer; electrons; fungi; heme; manganese; peroxidase; tryptophan
Abstract:
... Versatile peroxidase (VP) from the fungus Bjerkandera adusta is able to transport electrons from a catalytic tryptophan residue placed in the protein surface to the activated heme prosthetic group by a long range electron transfer (LRET) process. In this work, the protein surface of VP was covalently modified with tryptophan (W-VP) in order to increase the number of catalytic sites on the protein ...
DOI:
10.1016/j.molcatb.2015.12.001