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You searched for: Journal name Journal of natural products Remove constraint Journal name: Journal of natural products Subject term liquid chromatography Remove constraint Subject term: liquid chromatography
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1. Absolute Configuration and Pharmacology of the Poison Frog Alkaloid Phantasmidine

Author:
Richard W. Fitch; Barry B. Snider; Quan Zhou; Bruce M. Foxman; Anshul A. Pandya; Jerrel L. Yakel; Thao T. Olson; Nour Al-Muhtasib; Yingxian Xiao; Kevin D. Welch; Kip E. Panter
Source:
Journal of natural products 2018 v.81 no.4 pp. 1029-1035
ISSN:
1520-6025
Subject:
Epipedobates; agonists; carbon; enantiomers; enantioselectivity; liquid chromatography; mass spectrometry; nicotine; nicotinic receptors; pharmacology; poisonous frogs; therapeutics; toxicity
Abstract:
... Phantasmidine, a rigid congener of the well-known nicotinic acetylcholine receptor agonist epibatidine, is found in the same species of poison frog (Epipedobates anthonyi). Natural phantasmidine was found to be a 4:1 scalemic mixture, enriched in the (2aR,4aS,9aS) enantiomer by chiral-phase LC-MS comparison to the synthetic enantiomers whose absolute configurations were previously established by M ...
DOI:
10.1021/acs.jnatprod.8b00062
PubMed:
29671588
PubMed Central:
PMC7142328

2. Analytical Method To Detect and Quantify Avocatin B in Hass Avocado Seed and Pulp Matter

Author:
Nawaz Ahmed; Richard W. Smith; Juan J. Aristizabal Henao; Ken D. Stark; Paul A. Spagnuolo
Source:
Journal of natural products 2018 v.81 no.4 pp. 818-824
ISSN:
1520-6025
Subject:
antineoplastic activity; avocados; fatty alcohols; fruit pulp; gas chromatography-mass spectrometry; leukemia; liquid chromatography; nuclear magnetic resonance spectroscopy; quality control; seeds; stem cells
Abstract:
... Avocatin B, an avocado-derived compound mixture, was demonstrated recently to possess potent anticancer activity by selectively targeting and eliminating leukemia stem cells. Avocatin B is a mixture of avocadene and avocadyne, two 17-carbon polyhydroxylated fatty alcohols (PFAs), first discovered in avocado seeds; their quantities in avocado pulp are unknown. Analytical methods to detect avocado s ...
DOI:
10.1021/acs.jnatprod.7b00914

3. Antibacterial Cyclic Lipopeptide Enamidonins with an Enamide-Linked Acyl Chain from a Streptomyces Species

Author:
Sangkeun Son; Sung-Kyun Ko; Seung Min Kim; Eun Kim; Gil Soo Kim; Byeongsan Lee; In-Ja Ryoo; Won-Gon Kim; Jung-Sook Lee; Young-Soo Hong; Jae-Hyuk Jang; Jong Seog Ahn
Source:
Journal of natural products 2018 v.81 no.11 pp. 2462-2469
ISSN:
1520-6025
Subject:
Gram-positive bacteria; Staphylococcus aureus; Streptomyces; acetone; amino acids; antibacterial properties; antibiotic resistance; derivatization; linkage groups; lipopeptides; liquid chromatography; mass spectrometry; methicillin; nuclear magnetic resonance spectroscopy; Korean Peninsula
Abstract:
... Three cyclic lipopeptides, including one known (1) and two new (2 and 3) compounds, that possess the rare enamide linkage group were discovered from Streptomyces sp. KCB14A132, an actinobacterium isolated from a soil sample collected from Jeung Island, Korea. The NMR and MS-based characterization showed that they differed in the amino acid residues in the peptide backbone. Application of Marfey’s ...
DOI:
10.1021/acs.jnatprod.8b00497

4. Antimicrobial Peptides from Skin Secretions of Hypsiboas pulchellus (Anura: Hylidae)

Author:
Siano Alvaro; Húmpola María Verónica; de Oliveira Eliandre; Albericio Fernando; Simonetta Arturo C.; Lajmanovich Rafael; Tonarelli Georgina G.
Source:
Journal of natural products 2014 v.77 no.4 pp. 831-841
ISSN:
1520-6025
Subject:
Escherichia coli; Hypsiboas; Staphylococcus aureus; amphibians; antimicrobial peptides; antimicrobial properties; liquid chromatography; synthesis; synthetic peptides; tandem mass spectrometry; Argentina
Abstract:
... The skin of many amphibians produces a large repertoire of antimicrobial peptides that are crucial in the first line of defense against microbial invasion. Despite the immense richness of wild amphibians in Argentina, knowledge about peptides with antimicrobial properties is limited to a few species. Here we used LC-MS-MS to analyze samples of Hypsiboas pulchellus skin with the aim to identify ant ...
DOI:
10.1021/np4009317

5. Antiproliferative Cardenolides from the Aerial Parts of Pergularia tomentosa

Author:
Seyed Hamzeh Hosseini; Milena Masullo; Antonietta Cerulli; Stefania Martucciello; Mahdi Ayyari; Cosimo Pizza; Sonia Piacente
Source:
Journal of natural products 2019 v.82 no.1 pp. 74-79
ISSN:
1520-6025
Subject:
Pergularia; aerial parts; cardenolides; cell cycle; flavonols; glycosides; human cell lines; inhibitory concentration 50; liquid chromatography; mass spectrometry; methanol; neoplasm cells; nuclear magnetic resonance spectroscopy; prostatic neoplasms; roots; spectral analysis
Abstract:
... The LC-MS analysis of the MeOH extract of the aerial parts of Pergularia tomentosa led to the isolation of 23 compounds, of which the structures were elucidated unambiguously by NMR spectroscopic data analysis. Three new doubly linked cardenolides (4, 13, 14) along with several known cardenolides (1–3, 5, 7, 8, 15–23) and flavonol glycosides (6, 9–12) were identified. LC-HRESIMS analysis, in the n ...
DOI:
10.1021/acs.jnatprod.8b00630

6. Applicability of a Blood–Brain Barrier Specific Artificial Membrane Permeability Assay at the Early Stage of Natural Product-Based CNS Drug Discovery

Author:
Könczöl Árpád; Müller Judit; Földes Emília; Béni Zoltán; Végh Krisztina; Kéry Ágnes; Balogh György T.
Source:
Journal of natural products 2013 v.76 no.4 pp. 655-663
ISSN:
1520-6025
Subject:
Corydalis cava; Salvia officinalis; Tanacetum parthenium; Vinca major; blood-brain barrier; brain; drugs; liquid chromatography; mass spectrometry; membrane permeability; nuclear magnetic resonance spectroscopy; plant extracts; screening
Abstract:
... While numerous natural products (NPs) possess activity on central nervous system (CNS) targets, there has been no analytical approach to effectively identify compounds with high brain penetration potential in complex mixtures at the early stage of drug discovery. To overcome this issue, the performance of an in vitro parallel artificial membrane permeability assay for the blood–brain barrier (PAMP ...
DOI:
10.1021/np300882f

7. Bacilotetrins A and B, Anti-Staphylococcal Cyclic-Lipotetrapeptides from a Marine-Derived Bacillus subtilis

Author:
Tareq Fakir Shahidullah; Shin Hee Jae
Source:
Journal of natural products 2017 v.80 no.11 pp. 2889-2892
ISSN:
1520-6025
Subject:
Bacillus subtilis; bacteria; cytotoxicity; derivatization; fatty acids; fermentation; glutamic acid; lipopeptides; liquid chromatography; liver neoplasms; mass spectrometry; metabolomics; minimum inhibitory concentration; nuclear magnetic resonance spectroscopy; stereochemistry
Abstract:
... LC-MS and NMR spectroscopy guided metabolic profiling and dereplication of a crude extract obtained from the fermentation of a marine-derived bacterium, Bacillus subtilis, followed by chromatographic isolation yielded two new cyclic-lipotetrapeptides, bacilotetrins A (1) and B (2). Based on extensive 1D and 2D NMR and high-resolution ESIMS data analysis, the structures of 1 and 2 were elucidated, ...
DOI:
10.1021/acs.jnatprod.7b00356

8. Biosynthesis of Anatoxins in Cyanobacteria: Identification of the Carboxy-anatoxins as the Penultimate Biosynthetic Intermediates

Author:
Andreja Kust; Annick Méjean; Olivier Ploux
Source:
Journal of natural products 2020 v.83 no.1 pp. 142-151
ISSN:
1520-6025
Subject:
Cylindrospermum stagnale; Oscillatoria; acetates; anatoxins; biosynthesis; catalysts; catalytic activity; decarboxylation; hydrolysis; liquid chromatography; mass spectrometry; metabolites; neurotoxins; pH; polyketide synthases; proline; thioesters
Abstract:
... Anatoxin-a, homoanatoxin-a, and dihydroanatoxin-a are potent cyanobacterial neurotoxins. They are biosynthesized in cyanobacteria from proline and acetate by a pathway involving three polyketide synthases. We report the identification of carboxy-anatoxin-a, carboxy-homoanatoxin-a, and carboxy-dihydroanatoxin-a in acidic extracts of Cuspidothrix issatschenkoi CHARLIE-1, Oscillatoria sp. PCC 6506, a ...
DOI:
10.1021/acs.jnatprod.9b01121

9. Biosynthesis of Cylindrospermopsin in Cyanobacteria: Characterization of CyrJ the Sulfotransferase

Author:
Annick Méjean; Olivier Ploux
Source:
Journal of natural products 2021 v.84 no.2 pp. 408-416
ISSN:
1520-6025
Subject:
Escherichia coli; Oscillatoria; biosynthesis; culture media; cylindrospermopsin; genes; genetic vectors; kinetics; liquid chromatography; metabolites; sulfonates; tandem mass spectrometry
Abstract:
... 7-Deoxy-desulfo-cylindrospermopsin was purified at small-scale from the supernatant of a culture of the cyanobacterium Oscillatoria sp. PCC 10702. This metabolite was obtained in a pure form using a three-step chromatographic procedure, and its identity was confirmed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). LC–MS quantification showed that this metabolite was excreted in the c ...
DOI:
10.1021/acs.jnatprod.0c01089

10. Biotransformation of Phenolic 1-Benzyl-N-methyltetrahydroisoquinolines in Plant Cell Cultures Followed by LC/NMR, LC/MS, and LC/CD

Author:
Cui, W.; Iwasa, K.; Sugiura, M.; Takeuchi, A.; Tode, C.; Nishiyama, Y.; Moriyasu, M.; Tokuda, H.; Takeda, K.
Source:
Journal of natural products 2007 v.70 no.11 pp. 1771-1778
ISSN:
0163-3864
Subject:
phenolic compounds; isoquinolines; isoquinoline alkaloids; biotransformation; Corydalis; Macleaya; cultured cells; liquid chromatography; nuclear magnetic resonance spectroscopy
DOI:
10.1021/np068060r
PubMed:
17988098

11. Chemodiversity of Ladder-Frame Prymnesin Polyethers in Prymnesium parvum

Author:
Rasmussen Silas Anselm; Meier Sebastian; Andersen Nikolaj Gedsted; Blossom Hannah Eva; Duus Jens Øllgaard; Nielsen Kristian Fog; Hansen Per Juel; Larsen Thomas Ostenfeld
Source:
Journal of natural products 2016 v.79 no.9 pp. 2250-2256
ISSN:
1520-6025
Subject:
Prymnesium parvum; chlorination; economic impact; fish kills; glycosylation; liquid chromatography; mass spectrometry; microalgae; stable isotopes; toxicity; toxins
Abstract:
... Blooms of the microalga Prymnesium parvum cause devastating fish kills worldwide, which are suspected to be caused by the supersized ladder-frame polyether toxins prymnesin-1 and -2. These toxins have, however, only been detected from P. parvum in rare cases since they were originally described two decades ago. Here, we report the isolation and characterization of a novel B-type prymnesin, based o ...
DOI:
10.1021/acs.jnatprod.6b00345

12. Chloramphenicol Derivatives with Antibacterial Activity Identified by Functional Metagenomics

Author:
Shamima Nasrin; Suresh Ganji; Kavita S. Kakirde; Melissa R. Jacob; Mei Wang; Ranga Rao Ravu; Paul A. Cobine; Ikhlas A. Khan; Cheng-Cang Wu; David A. Mead; Xing-Cong Li; Mark R. Liles
Source:
Journal of natural products 2018 v.81 no.6 pp. 1321-1332
ISSN:
1520-6025
Subject:
Escherichia coli; Mycobacterium intracellulare; Mycobacterium tuberculosis; antibacterial properties; antibiotic resistance; bacterial artificial chromosomes; chloramphenicol; clones; derivatization; esterases; genes; liquid chromatography; mass spectrometry; metagenomics; methicillin; methicillin-resistant Staphylococcus aureus; plasmids; soil
Abstract:
... A functional metagenomic approach identified novel and diverse soil-derived DNAs encoding inhibitors to methicillin-resistant Staphylococcus aureus (MRSA). A metagenomic DNA soil library containing 19 200 recombinant Escherichia coli BAC clones with 100 Kb average insert size was screened for antibiotic activity. Twenty-seven clones inhibited MRSA, seven of which were found by LC-MS to possess mod ...
DOI:
10.1021/acs.jnatprod.7b00903
CHORUS:
10.1021/acs.jnatprod.7b00903

13. Colletopeptides A–D, Anti-inflammatory Cyclic Tridepsipeptides from the Plant Endophytic Fungus Colletotrichum sp. S8

Author:
Li Feng; Jia Wang; Shuai Liu; Xue-Jia Zhang; Qi-Rui Bi; Yan-Yun Hu; Zhe Wang; Ning-Hua Tan
Source:
Journal of natural products 2019 v.82 no.6 pp. 1434-1441
ISSN:
1520-6025
Subject:
Colletotrichum; IKappaB kinase; Rubia; X-ray diffraction; dodecanoic acid; endophytes; fungi; inhibitory concentration 50; interleukin-6; liquid chromatography; macrophages; nitric oxide; phosphorylation; proteins; spectral analysis; stems; tumor necrosis factor-alpha
Abstract:
... Four new hybrid peptide-polyketide cyclic tridepsipeptides, colletopeptides A–D (1–4), were isolated and characterized from the endophytic fungus Colletotrichum sp. S8 derived from the stems of Rubia podantha with the guidance of LC-UV-MS detection. Their structures were elucidated by extensive spectroscopic analysis and X-ray crystallography. Compounds 1–4 are rare natural 12-membered cyclic trid ...
DOI:
10.1021/acs.jnatprod.8b00829

14. Combination of LC-MS and LC-NMR as a tool for the structure determination of natural products

Author:
Vogler, B.; Klaiber, I.; Roos, G.; Walter, C.U.; Hiller, W.; Sandor, P.; Kraus, W.
Source:
Journal of natural products 1998 v.61 no.2 pp. 175-178
ISSN:
0163-3864
Subject:
spectral analysis; chemical structure; liquid chromatography; mass spectrometry; nuclear magnetic resonance spectroscopy; chemical constituents of plants
DOI:
10.1021/np970416z

15. Cryptic Species Account for the Seemingly Idiosyncratic Secondary Metabolism of Sarcophyton glaucum Specimens Collected in Palau

Author:
Katherine N. Maloney; Ryan T. Botts; Taylor S. Davis; Bethany K. Okada; Elizabeth M. Maloney; Christopher A. Leber; Oscar Alvarado; Charlie Brayton; Andrés Mauricio Caraballo-Rodríguez; Jason V. Chari; Brent Chicoine; J. Chance Crompton; Sydney R. Davis; Samantha M. Gromek; Viqqi Kurnianda; Kim Quach; Robert M. Samples; Vincent Shieh; Camille M. Sultana; Junichi Tanaka; Pieter C. Dorrestein; Marcy J. Balunas; Catherine S. McFadden
Source:
Journal of natural products 2020 v.83 no.3 pp. 693-705
ISSN:
1520-6025
Subject:
Sarcophyton; biochemical polymorphism; chemistry; corals; cryptic species; discriminant analysis; diterpenoids; flame ionization; interspecific variation; liquid chromatography; mass spectrometry; metabolism; metabolites; phylogeny; principal component analysis; Palau
Abstract:
... Sarcophyton glaucum is one of the most abundant and chemically studied soft corals with over 100 natural products reported in the literature, primarily cembrane diterpenoids. Yet, wide variation in the chemistry observed from S. glaucum over the past 50 years has led to its reputation as a capricious producer of bioactive metabolites. Recent molecular phylogenetic analysis revealed that S. glaucum ...
DOI:
10.1021/acs.jnatprod.9b01128

16. Cyclotides from the Indian Medicinal Plant Viola odorata (Banafsha): Identification and Characterization

Author:
Narayani M.; Chadha Anju; Srivastava Smita
Source:
Journal of natural products 2017 v.80 no.7 pp. 1972-1980
ISSN:
1520-6025
Subject:
Viola odorata; alkylation; digestion; flowers; liquid chromatography; medicinal plants; peptides; petioles; roots; tandem mass spectrometry; India
Abstract:
... Cyclotides are cyclic cystine knotted macrocyclic plant peptides that have several promising applications. This study was undertaken to detect and identify known and new cyclotides in Viola odorata, a commercially important medicinal plant, from three geographical locations in India. The number of cyclotides in the plant varied with the tissue (leaves, petioles, flowers, runners, and roots) and wi ...
DOI:
10.1021/acs.jnatprod.6b01004

17. Cyperane-Type and Related (Nor)Sesquiterpenoids from the Root Bark of Acanthopanax gracilistylus and Their Inhibitory Effects on Nitric Oxide Production

Author:
Hong-Bo Xu; Tong-Hua Yang; Pei Xie; Zhi-Shu Tang; Huai-Li Xu; Chong Deng; Yan-Ni Liang; Rui Zhou; Shi-Jun Liu; Yu Zhang
Source:
Journal of natural products 2020 v.83 no.5 pp. 1453-1460
ISSN:
1520-6025
Subject:
Eleutherococcus gracilistylus; X-ray diffraction; bark; bioassays; biosynthesis; humans; inducible nitric oxide synthase; inhibitory concentration 50; lactones; liquid chromatography; nitric oxide; nuclear magnetic resonance spectroscopy; sesquiterpenoids
Abstract:
... An enantiomeric pair of rare cyperane-type sesquiterpenoids, (+)- and (−)-gracilistones C (1a, 1b), together with a novel norsesquiterpenoid, gracilistone D (2), bearing a bicyclic lactone system were isolated from the root bark of Acanthopanax gracilistylus using LC-MS-IT-TOF analyses. The structures and absolute configurations of 1a, 1b, and 2 were elucidated by 1D and 2D NMR spectroscopy, X-ray ...
DOI:
10.1021/acs.jnatprod.9b00913

18. Cytotoxic Glycosylated Fatty Acid Amides from a Stelletta sp. Marine Sponge

Author:
Peddie Victoria; Takada Kentaro; Okuda Shujiro; Ise Yuji; Morii Yasuhiro; Yamawaki Nobuhiro; Takatani Tomohiro; Arakawa Osamu; Okada Shigeru; Matsunaga Shigeki
Source:
Journal of natural products 2015 v.78 no.11 pp. 2808-2813
ISSN:
1520-6025
Subject:
Porifera; amides; cytotoxicity; derivatization; fatty acids; glycosylation; inhibitory concentration 50; liquid chromatography; mass spectrometry; models; nuclear magnetic resonance spectroscopy
Abstract:
... We have discovered new glycosylated fatty acid amides, stellettosides, from a Stelletta sp. marine sponge. They were detected through LC-MS analysis of the extract combined with the cytotoxicity assay of the prefractionated sample. Their planar structures were determined by analyses of the NMR and tandem FABMS data. Stellettosides A1 and A2 (1 and 2) as well as stellettosides B1–B4 (3–6) were obta ...
DOI:
10.1021/acs.jnatprod.5b00795

19. Cytotoxic Microcolin Lipopeptides from the Marine Cyanobacterium Moorea producens

Author:
Hao-Bing Yu; Evgenia Glukhov; Yueying Li; Arihiro Iwasaki; Lena Gerwick; Pieter C. Dorrestein; Bing-Hua Jiao; William H. Gerwick
Source:
Journal of natural products 2019 v.82 no.9 pp. 2608-2619
ISSN:
1520-6025
Subject:
Cyanobacterium (genus); Moorea producens; amino acids; catalytic activity; cytotoxicity; hydrogenation; inhibitory concentration 50; lipopeptides; liquid chromatography; lung neoplasms; mass spectrometry; metabolites; neoplasm cells; structure-activity relationships
Abstract:
... Nine new linear lipopeptides, microcolins E–M (1–9), together with four known related compounds, microcolins A–D (10–13), were isolated from the marine cyanobacterium Moorea producens using bioassay-guided and LC-MS/MS molecular networking approaches. Catalytic hydrogenation of microcolins A–D (10–13) yielded two known compounds, 3,4-dihydromicrocolins A and B (14, 15), and two new derivatives, 3, ...
DOI:
10.1021/acs.jnatprod.9b00549

20. Dammarane-Type Triterpenoids from the Roots of Rhus chinensis and Their Preventive Effects on Zebrafish Heart Failure and Thrombosis

Author:
Miao Ye; Wen Xu; Da-Qing He; Xian Wu; Wen-Fang Lai; Xiao-Qin Zhang; Yu Lin; Wei Xu; Xu-Wen Li
Source:
Journal of natural products 2020 v.83 no.2 pp. 362-373
ISSN:
1520-6025
Subject:
Danio rerio; Oriental traditional medicine; Rhus; bioassays; blood flow; cardiac output; coronary vessels; drugs; heart; heart failure; heart rate; liquid chromatography; mass spectrometry; moieties; quantum mechanics; roots; spectral analysis; therapeutics; thrombosis; triterpenoids
Abstract:
... Eight new dammarane-type triterpenoids (1–8), together with a related known analogue (9), were isolated from the roots of Rhus chinensis, a traditional Chinese medicine for treating coronary artery heart disease, guided by LC-MS analysis. Their structures were elucidated based on extensive spectroscopic analysis and quantum chemical calculations. Notably, compounds 1–7 and 9 possess an unusual 17α ...
DOI:
10.1021/acs.jnatprod.9b00857

21. Deinococcucins A–D, Aminoglycolipids from Deinococcus sp., a Gut Bacterium of the Carpenter Ant Camponotus japonicus

Author:
Shin Bora; Park So Hyun; Kim Byung-Yong; Jo Shin-Il; Lee Sang Kook; Shin Jongheon; Oh Dong-Chan
Source:
Journal of natural products 2017 v.80 no.11 pp. 2910-2916
ISSN:
1520-6025
Subject:
Camponotus; Deinococcus; NAD(P)H dehydrogenase (quinone); carpenter ants; chemical bonding; digestive system; gas chromatography-mass spectrometry; glucosamine; intestinal microorganisms; liquid chromatography; mice; moieties; nuclear magnetic resonance spectroscopy; olefin; spectral analysis
Abstract:
... Four new aminoglycolipids, deinococcucins A–D (1–4), were discovered from a Deinococcus sp. strain isolated from the gut of queen carpenter ants, Camponotus japonicus. The structures of deinococcucins A–D were elucidated as a combination of N-acetyl glucosamine, 2,3-dihydroxypropanoic acid, and an alkyl amine with a C₁₆ or C₁₇ hydrocarbon chain primarily based on 1D and 2D NMR and mass spectroscop ...
DOI:
10.1021/acs.jnatprod.7b00426

22. Diarylheptanoids from Dioscorea villosa (Wild Yam)

Author:
Dong Shi-Hui; Nikolić Dejan; Simmler Charlotte; Qiu Feng; van Breemen Richard B.; Soejarto Djaja D.; Pauli Guido F.; Chen Shao-Nong
Source:
Journal of natural products 2012 v.75 no.12 pp. 2168-2177
ISSN:
1520-6025
Subject:
Dioscorea villosa; chemical constituents of plants; fractionation; liquid chromatography; mass spectrometry; metabolome; metabolomics; nuclear magnetic resonance spectroscopy; plant extracts; secondary metabolites; yams
Abstract:
... A fractionation methodology aimed at the metabolomic mining of new phytoconstituents for the widely used botanical, wild yam (Dioscorea villosa), makes use of 1D qHNMR and 2D NMR profiles along the preparative fractionation pathway. This quantifiable and structural guidance led to the isolation of 14 diarylheptanoids (1–14), including five new compounds (1–5) with a tetrahydropyrano core skeleton. ...
DOI:
10.1021/np300603z
PubMed:
23245349
PubMed Central:
PMC3710746

23. Dihydroanatoxin-a Is Biosynthesized from Proline in Cylindrospermum stagnale PCC 7417: Isotopic Incorporation Experiments and Mass Spectrometry Analysis

Author:
Méjean Annick; Dalle Klervi; Paci Guillaume; Bouchonnet Stéphane; Mann Stéphane; Pichon Valérie; Ploux Olivier
Source:
Journal of natural products 2016 v.79 no.7 pp. 1775-1782
ISSN:
1520-6025
Subject:
Cylindrospermum stagnale; Oscillatoria; anatoxins; carbon; gas chromatography-mass spectrometry; ionization; isotope labeling; liquid chromatography; multigene family; proline
Abstract:
... LC-MS and GC-MS analytical conditions have been developed to detect the cis- and trans-epimers (relative configuration of the carbon bearing the acetyl or propionyl group) of dihydroanatoxin-a and dihydrohomoanatoxin-a, in biological samples. These compounds epimerize under acidic conditions, yielding a major species that was tentatively assigned as the cis-epimer. Cylindrospermum stagnale PCC 741 ...
DOI:
10.1021/acs.jnatprod.6b00189

24. Discovery of Venturicidin Congeners and Identification of the Biosynthetic Gene Cluster from Streptomyces sp. NRRL S-4

Author:
Huanhuan Li; Mengxue Zhang; Hongji Li; Hai Yu; Shuo Chen; Wenhui Wu; Peng Sun
Source:
Journal of natural products 2020 v.84 no.1 pp. 110-119
ISSN:
1520-6025
Subject:
Streptomyces; biochemical pathways; bioinformatics; biosynthesis; cytotoxicity; glycosylation; leukemia; liquid chromatography; macrolides; mass spectrometry; multigene family
Abstract:
... Chemical screening of Streptomyces sp. NRRL S-4 with liquid chromatography–mass spectrometry (LC–MS) and the following chromatographic isolation led to the discovery of four 20-membered macrolides, venturicidin A (4) and three new congeners venturicidins D–F (1–3). Genome sequencing of strain S-4 revealed the presence of a biosynthetic gene cluster (BGC) encoding glycosylated type I polyketides (P ...
DOI:
10.1021/acs.jnatprod.0c01177

25. Do Plant Cyclotides Have Potential As Immunosuppressant Peptides?

Author:
Gründemann Carsten; Koehbach Johannes; Huber Roman; Gruber Christian W
Source:
Journal of natural products 2012 v.75 no.2 pp. 167-174
ISSN:
1520-6025
Subject:
Oldenlandia; cytotoxicity; databases; drugs; humans; liquid chromatography; lymphocytes; mass spectrometry; peptides; plant extracts; sequence analysis
Abstract:
... Cyclotides are an abundant and diverse group of ribosomally synthesized plant peptides containing a cyclic cystine-knotted structure that confers them with remarkable stability. They are explored for their distribution in plants, although little is known about the individual peptide content of a single species. Therefore, we chemically analyzed the crude extract of the coffee-family plant Oldenlan ...
DOI:
10.1021/np200722w
PubMed:
22272797
PubMed Central:
PMC3399773

26. Epigenetic Modulation of Endophytic Eupenicillium sp. LG41 by a Histone Deacetylase Inhibitor for Production of Decalin-Containing Compounds

Author:
Li Gang; Kusari Souvik; Golz Christopher; Laatsch Hartmut; Strohmann Carsten; Spiteller Michael
Source:
Journal of natural products 2017 v.80 no.4 pp. 983-988
ISSN:
1520-6025
Subject:
Eupenicillium; Staphylococcus aureus; X-ray diffraction; Xanthium; chemical equilibrium; cis-trans isomers; cytotoxicity; endophytes; epigenetics; fungi; histone deacetylase; humans; liquid chromatography; mass spectrometry; medicinal plants; neoplasm cells; nicotinamide; nuclear magnetic resonance spectroscopy; spectral analysis; stereochemistry
Abstract:
... An endophytic fungus, Eupenicillium sp. LG41, isolated from the Chinese medicinal plant Xanthium sibiricum, was subjected to epigenetic modulation using an NAD⁺-dependent histone deacetylase (HDAC) inhibitor, nicotinamide. Epigenetic stimulation of the endophyte led to enhanced production of two new decalin-containing compounds, eupenicinicols C and D (3 and 4), along with two biosynthetically rel ...
DOI:
10.1021/acs.jnatprod.6b00997

27. Eudesmanolides from Dimerostemma vestitum

Author:
Stefani, R.; Eberlin, M.N.; Tomazela, D.M.; Costa, F.B. da
Source:
Journal of natural products 2003 v.66 no.3 pp. 401-403
ISSN:
0163-3864
Subject:
eudesmanolides; photochemistry; plant extracts; epoxides; stereoisomers; spectral analysis; Asteraceae; liquid chromatography; leaves; Brazil
DOI:
10.1021/np020304u
PubMed:
12662100

28. Evaluation of Ion Mobility Spectrometry for Improving Constitutional Assignment in Natural Product Mixtures

Author:
Fausto Carnevale Neto; Trevor N. Clark; Norberto P. Lopes; Roger G. Linington
Source:
Journal of natural products 2022 v.85 no.3 pp. 519-529
ISSN:
1520-6025
Subject:
liquid chromatography; metabolites; solvents; tandem mass spectrometry
Abstract:
... The comprehensive chemical characterization of biological samples remains a central challenge in the field of natural products. Conventional workflows using liquid chromatography (LC)-coupled high-resolution tandem mass spectrometry (MS/MS or MS²) allow the detection of relevant small molecules while providing diagnostic fragment ions for their structural assignment. Still, many natural product ex ...
DOI:
10.1021/acs.jnatprod.1c01048

29. Evaluation of the Intestinal Absorption Mechanism of Casearin X in Caco-2 Cells with Modified Carboxylesterase Activity

Author:
Moreira da Silva Rodrigo; Verjee Sheela; de Gaitani Cristiane Masetto; Moraes de Oliveira Anderson Rodrigo; Pires Bueno Paula Carolina; Cavalheiro Alberto José; Peporine Lopes Norberto; Butterweck Veronika
Source:
Journal of natural products 2016 v.79 no.4 pp. 1084-1090
ISSN:
1520-6025
Subject:
Casearia; active transport; carboxylesterase; cytotoxicity; diterpenoids; enzymatic hydrolysis; human cell lines; humans; intestinal absorption; intestines; leaves; liquid chromatography; mass spectrometry; metabolism; permeability
Abstract:
... The clerodane diterpene casearin X (1), isolated from the leaves of Casearia sylvestris, is a potential new drug candidate due to its potent in vitro cytotoxic activity. In this work, the intestinal absorption mechanism of 1 was evaluated using Caco-2 cells with and without active carboxylesterases (CES). An LC-MS method was developed and validated for the quantification of 1. The estimation of pe ...
DOI:
10.1021/acs.jnatprod.5b01139

30. Expanding the Scope of Detectable Microbial Natural Products by Complementary Analytical Methods and Cultivation Systems

Author:
Chantal D. Bader; Patrick A. Haack; Fabian Panter; Daniel Krug; Rolf Müller
Source:
Journal of natural products 2021 v.84 no.2 pp. 268-277
ISSN:
1520-6025
Subject:
agar; genome; liquid chromatography; liquids; mass spectrometry; metabolism; metabolomics; myxobacteria; secondary metabolites; spectrometers; statistical analysis
Abstract:
... Recent advances in genome sequencing have unveiled a large discrepancy between the genome-encoded capacity of microorganisms to produce secondary metabolites and the number detected. In this work, a two-platform mass spectrometry analysis for the comprehensive secondary metabolomics characterization of nine myxobacterial strains, focusing on extending the range of detectable secondary metabolites ...
DOI:
10.1021/acs.jnatprod.0c00942

31. Experimental Evidence of the Presence of Bimolecular Caffeine/Catechin Complexes in Green Tea Extracts

Author:
Luisa Mattoli; Valentino Mercati; Michela Burico; Stella Bedont; Marina Porchia; Francesco Tisato; Sara D’Aronco; Sara Crotti; Marco Agostini; Pietro Traldi
Source:
Journal of natural products 2018 v.81 no.11 pp. 2338-2347
ISSN:
1520-6025
Subject:
caffeine; catechin; chemistry; green tea; ions; liquid chromatography; nuclear magnetic resonance spectroscopy; tandem mass spectrometry
Abstract:
... A hypothesis on the peculiar pharmacological behavior of biologically active natural compounds is based on the occurrence of molecular interactions originating from the high complexity of the natural matrix, following the rules of supramolecular chemistry. In this context, some investigations were performed to establish unequivocally the presence of caffeine/catechin complexes in green tea extract ...
DOI:
10.1021/acs.jnatprod.8b00168

32. Glycosides of the Neurotoxin Tutin in Toxic Honeys Are from Coriaria arborea Phloem Sap, Not Insect Metabolism

Author:
Oliver C. Watkins; Nigel I. Joyce; Nick Gould; Nigel B. Perry
Source:
Journal of natural products 2018 v.81 no.4 pp. 1116-1120
ISSN:
1520-6025
Subject:
Coriaria; bees; glycosides; honey; honeydew; leaves; liquid chromatography; mass spectrometry; metabolism; neurotoxins; phloem; sap; sucrose; toxicity
Abstract:
... Some honeys contain the neurotoxin tutin (1) plus hyenanchin (2), 2-(β-d-glucopyranosyl)tutin (3), and 2-[6′-(α-d-glucopyranosyl)-β-d-glucopyranosyl]tutin (4). These honeys are made by bees collecting honeydew from passionvine hoppers feeding on the sap of tutu plants (Coriaria spp.). We report a LC-MS study showing that all these picrotoxanes are of plant, not insect, origin. Hyenanchin was barel ...
DOI:
10.1021/acs.jnatprod.8b00120

33. High-Resolution Liquid Chromatography–Mass Spectrometry-Based Metabolomic Discrimination of Citrus-Type Crude Drugs and Comparison with Nuclear Magnetic Resonance Spectroscopy-Based Metabolomics

Author:
Takashi Tsujimoto; Taichi Yoshitomi; Takuro Maruyama; Yutaka Yamamoto; Takashi Hakamatsuka; Nahoko Uchiyama
Source:
Journal of natural products 2019 v.82 no.8 pp. 2116-2123
ISSN:
1520-6025
Subject:
coumarin; drugs; glucosides; liquid chromatography; mass spectrometry; metabolome; metabolomics; multivariate analysis; naringin; neohesperidin; nuclear magnetic resonance spectroscopy; secondary metabolites; stable isotopes
Abstract:
... Five Citrus-type crude drugs (40 samples) were classified using liquid chromatography–mass spectrometry (LC-MS)-based metabolomics. The following six flavonoid derivatives were identified as contributors from the loading plots of multivariate analysis: naringin (1), neohesperidin (2), neoeriocitrin (3), narirutin (9), hesperidin (10), and 3,5,6,7,8,3′,4′-heptamethoxyflavone (12). Three coumarin de ...
DOI:
10.1021/acs.jnatprod.8b00977

34. Identification of Narciclasine as an in Vitro Anti-Inflammatory Component of Cyrtanthus contractus by Correlation-Based Metabolomics

Author:
Lucie Rárová; Bhekumthetho Ncube; Johannes Van Staden; Robert Fürst; Miroslav Strnad; Jiri Gruz
Source:
Journal of natural products 2019 v.82 no.5 pp. 1372-1376
ISSN:
1520-6025
Subject:
Cyrtanthus contractus; anti-inflammatory activity; bulbs; liquid chromatography; mass spectrometry; metabolites; metabolomics
Abstract:
... In this study, an extract from the bulbs of Cyrtanthus contractus showed strong anti-inflammatory activity in vitro. The extract was partially separated into 14 fractions and analyzed by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry metabolomics, and the correlation coefficients were calculated between biological activities and metabolite levels. As a res ...
DOI:
10.1021/acs.jnatprod.8b00973

35. Inducing Secondary Metabolite Production by Combined Culture of Talaromyces aculeatus and Penicillium variabile

Author:
Zhenzhen Zhang; Xueqian He; Guojian Zhang; Qian Che; Tianjiao Zhu; Qianqun Gu; Dehai Li
Source:
Journal of natural products 2017 v.80 no.12 pp. 3167-3171
ISSN:
1520-6025
Subject:
Penicillium variabile; Talaromyces; anticarcinogenic activity; circular dichroism spectroscopy; cytotoxicity; density functional theory; fungi; human cell lines; humans; inhibitory concentration 50; liquid chromatography; mass spectrometry; mixed culture; neoplasm cells; nuclear magnetic resonance spectroscopy; polyketides; secondary metabolites
Abstract:
... Four new polyketides, penitalarins A–C (1–3) and nafuredin B (4), together with the known biogenetically related nafuredin A (5) were isolated from a mixed culture of a deep-sea-derived fungus Talaromyces aculeatus and a mangrove-derived fungus Penicillium variabile. Liquid chromatography/mass spectrometry analysis showed that none of compounds 1–5 was produced by either of the two fungi when cult ...
DOI:
10.1021/acs.jnatprod.7b00417

36. Inhibition of Human Prolyl Oligopeptidase Activity by the Cyclotide Psysol 2 Isolated from Psychotria solitudinum

Author:
Hellinger Roland; Koehbach Johannes; Puigpinós Albert; Clark Richard J.; Tarragó Teresa; Giralt Ernest; Gruber Christian W.
Source:
Journal of natural products 2015 v.78 no.5 pp. 1073-1082
ISSN:
1520-6025
Subject:
Momordica; Parkinson disease; Psychotria; antiretroviral agents; bioactive properties; bioavailability; chymotrypsin; cognition; fractionation; humans; immunosuppression; inhibitory concentration 50; liquid chromatography; memory; peptides; proteolysis; schizophrenia; trypsin
Abstract:
... Cyclotides are head-to-tail cyclized peptides comprising a stabilizing cystine-knot motif. To date, they are well known for their diverse bioactivities such as anti-HIV and immunosuppressive properties. Yet little is known about specific molecular mechanisms, in particular the interaction of cyclotides with cellular protein targets. Native and synthetic cyclotide-like peptides from Momordica plant ...
DOI:
10.1021/np501061t
PubMed:
25894999
PubMed Central:
PMC4444998

37. Investigation of Premyrsinane and Myrsinane Esters in Euphorbia cupanii and Euphobia pithyusa with MS2LDA and Combinatorial Molecular Network Annotation Propagation

Author:
Mélissa Nothias-Esposito; Louis Felix Nothias; Ricardo R. Da Silva; Pascal Retailleau; Zheng Zhang; Pieter Leyssen; Fanny Roussi; David Touboul; Julien Paolini; Pieter C. Dorrestein; Marc Litaudon
Source:
Journal of natural products 2019 v.82 no.6 pp. 1459-1470
ISSN:
1520-6025
Subject:
Chikungunya virus; Euphorbia; X-ray diffraction; databases; diterpenoids; esters; liquid chromatography; nuclear magnetic resonance spectroscopy; tandem mass spectrometry; virus replication
Abstract:
... The species Euphorbia pithyusa and Euphorbia cupanii are two closely related Mediterranean spurges for which their taxonomic relationships are still being debated. Herein, the diterpene ester content of E. cupanii was investigated using liquid chromatography coupled to tandem mass spectrometry. The use of molecular networking coupled to unsupervised substructure annotation (MS2LDA) indicated the p ...
DOI:
10.1021/acs.jnatprod.8b00916

38. Isolation and Structures of Pipecolidepsins A and B, Cytotoxic Cyclic Depsipeptides from the Madagascan Sponge Homophymia lamellosa

Author:
Coello Laura; Reyes Fernando; Martín María Jesús; Cuevas Carmen; Fernández Rogelio
Source:
Journal of natural products 2014 v.77 no.2 pp. 298-303
ISSN:
1520-6025
Subject:
amino acids; coasts; cytotoxicity; depsipeptides; derivatization; humans; hydrolysis; liquid chromatography; mass spectrometry; neoplasm cells; nuclear magnetic resonance spectroscopy; Madagascar
Abstract:
... Two new cyclic depsipeptides, pipecolidepsins A and B (1 and 2), have been isolated from the sponge Homophymia lamellosa collected off the coast of Madagascar. Their structures were determined by a combination of NMR experiments and by LC-MS analysis of the amino acid fragments obtained by hydrolysis and derivatization using Marfey’s reagent. In addition to several common amino acids, these peptid ...
DOI:
10.1021/np400888e

39. Isolation of 6-Deoxytetrodotoxin from the Pufferfish, Takifugu pardalis, and a Comparison of the Effects of the C-6 and C-11 Hydroxy Groups of Tetrodotoxin on Its Activity

Author:
Kudo Yuta; Finn Julian; Fukushima Kohei; Sakugawa Satsuki; Cho Yuko; Konoki Keiichi; Yotsu-Yamashita Mari
Source:
Journal of natural products 2014 v.77 no.4 pp. 1000-1004
ISSN:
1520-6025
Subject:
Nassarius; Takifugu pardalis; biochemical pathways; cytotoxicity; liquid chromatography; mass spectrometry; median effective concentration; mice; ouabain; snails; sodium channels; structure-activity relationships; tetrodotoxin
Abstract:
... Identification of new tetrodotoxin (TTX, 1) analogues would be significant in the elucidation of its biosynthetic pathway and a study of its structure–activity relationships. In this study, a new TTX analogue, 6-deoxyTTX (2), was isolated from the ovary of the pufferfish, Takifugu pardalis, and the structure was determined using spectroscopic methods. Compound 2 was also identified in other marine ...
DOI:
10.1021/np401097n

40. Isolation, Structure Elucidation, Relative LC-MS Response, and in Vitro Toxicity of Azaspiracids from the Dinoflagellate Azadinium spinosum

Author:
Kilcoyne Jane; Nulty Ciara; Jauffrais Thierry; McCarron Pearse; Herve Fabienne; Foley Barry; Rise Frode; Crain Sheila; Wilkins Alistair L.; Twiner Michael J.; Hess Philipp; Miles Christopher O.
Source:
Journal of natural products 2014 v.77 no.11 pp. 2465-2474
ISSN:
1520-6025
Subject:
Azadinium spinosum; T-lymphocytes; cytotoxicity; liquid chromatography; mass spectrometry; models; molecular weight; mussels; nuclear magnetic resonance spectroscopy; seawater; structure-activity relationships; toxicity testing
Abstract:
... We identified three new azaspiracids (AZAs) with molecular weights of 715, 815, and 829 (AZA33 (3), AZA34 (4), and AZA35, respectively) in mussels, seawater, and Azadinium spinosum culture. Approximately 700 μg of 3 and 250 μg of 4 were isolated from a bulk culture of A. spinosum, and their structures determined by MS and NMR spectroscopy. These compounds differ significantly at the carboxyl end o ...
DOI:
10.1021/np500555k

41. K-Targeted Metabolomic Analysis Extends Chemical Subtraction to DESIGNER Extracts: Selective Depletion of Extracts of Hops (Humulus lupulus)

Author:
Ramos Alvarenga René F.; Friesen J. Brent; Nikolić Dejan; Simmler Charlotte; Napolitano José G.; van Breemen Richard; Lankin David C.; McAlpine James B.; Pauli Guido F.; Chen Shao-Nong
Source:
Journal of natural products 2014 v.77 no.12 pp. 2595-2604
ISSN:
1520-6025
Subject:
Humulus lupulus; antagonism; equations; hops; ingredients; liquid chromatography; mass spectrometry; metabolites; nuclear magnetic resonance spectroscopy; partition coefficients; phenols; quantitative analysis; solvents; synergism
Abstract:
... This study introduces a flexible and compound targeted approach to Deplete and Enrich Select Ingredients to Generate Normalized Extract Resources, generating DESIGNER extracts, by means of chemical subtraction or augmentation of metabolites. Targeting metabolites based on their liquid–liquid partition coefficients (K values), K targeting uses countercurrent separation methodology to remove single ...
DOI:
10.1021/np500376g
PubMed:
25437744
PubMed Central:
PMC4280114

42. LC-MS- and 1H NMR-Based Metabolomic Analysis and in Vitro Toxicological Assessment of 43 Aristolochia Species

Author:
Michl Johanna; Kite Geoffrey C.; Wanke Stefan; Zierau Oliver; Vollmer Guenter; Neinhuis Christoph; Simmonds Monique S. J.; Heinrich Michael
Source:
Journal of natural products 2016 v.79 no.1 pp. 30-37
ISSN:
1520-6025
Subject:
Aristolochia; acids; cytotoxicity; genotoxicity; herbal medicines; humans; kidney neoplasms; kidneys; liquid chromatography; mass spectrometry; nephrotoxicity; renal failure; toxicity testing
Abstract:
... Species of Aristolochia are used as herbal medicines worldwide. They cause aristolochic acid nephropathy (AAN), a devastating disease associated with kidney failure and renal cancer. Aristolochic acids I and II (1 and 2) are considered to be responsible for these nephrotoxic and carcinogenic effects. A wide range of other aristolochic acid analogues (AAAs) exist, and their implication in AAN may h ...
DOI:
10.1021/acs.jnatprod.5b00556

43. LC-MS- and H NMR Spectroscopy-Guided Identification of Antifungal Diterpenoids from Sagittaria latifolia

Author:
Ranga Rao Ravu; Melissa R. Jacob; Cynthia Jeffries; Ying Tu; Shabana I. Khan; Ameeta K. Agarwal; R. Kiplin Guy; Larry A. Walker; Alice M. Clark; Xing-Cong Li
Source:
Journal of natural products 2015 v.78 no.9 pp. 2255-2259
ISSN:
0163-3864
Subject:
Cryptococcus gattii; Cryptococcus neoformans; Sagittaria latifolia; acid hydrolysis; animal pathogenic fungi; antifungal agents; chemical structure; chemotypes; diterpenoids; drugs; fractionation; inhibitory concentration 50; liquid chromatography; mass spectrometry; nuclear magnetic resonance spectroscopy; plant extracts; screening; spectral analysis
Abstract:
... A dereplication strategy using a combination of liquid chromatography-mass spectrometry (LC-MS) and proton nuclear magnetic resonance spectroscopy (1H NMR) to facilitate compound identification towards antifungal natural product discovery is presented. This analytical approach takes advantage of the simplicity of semi-purified column fractions (CFs) of plant extracts, which were generated by an au ...
Handle:
10113/62607
DOI:
10.1021/acs.jnatprod.5b00470
CHORUS:
10.1021/acs.jnatprod.5b00470

44. LC-MS-Guided Isolation of Insulin-Secretion-Promoting Monoterpenoid Carbazole Alkaloids from Murraya microphylla

Author:
Xiao-Li Ma; Jun Li; Jiao Zheng; Xiao-Pan Gu; Daneel Ferreira; Jordan K. Zjawiony; Ming-Bo Zhao; Xiao-Yu Guo; Peng-Fei Tu; Yong Jiang
Source:
Journal of natural products 2018 v.81 no.11 pp. 2371-2380
ISSN:
1520-6025
Subject:
Murraya; X-ray diffraction; alkaloids; cell lines; chemical bonding; enantiomers; ethanol; fractionation; insulin secretion; liquid chromatography; monoterpenoids; nuclear magnetic resonance spectroscopy
Abstract:
... Fifteen new structurally unique monoterpenoid carbazole alkaloids, including two pairs of epimers (1/2 and 3/4), three pairs of enantiomers (6a/6b, 7a/7b, and 8a/8b), and five optically pure analogues (5, 9–12), were obtained from a 95% aqueous EtOH extract of Murraya microphylla by a combination of bioassay- and LC-MS-guided fractionation procedures. Their structures were established based on NMR ...
DOI:
10.1021/acs.jnatprod.8b00338

45. Metabolomics Analysis Reveals that Ethylene and Methyl Jasmonate Regulate Different Branch Pathways to Promote the Accumulation of Terpenoid Indole Alkaloids in Catharanthus roseus

Author:
Xiao-Ning Zhang; Jia Liu; Yang Liu; Yu Wang; Ann Abozeid; Zhi-Guo Yu; Zhong-Hua Tang
Source:
Journal of natural products 2018 v.81 no.2 pp. 335-342
ISSN:
1520-6025
Subject:
Catharanthus roseus; biochemical pathways; biosynthesis; ethylene; liquid chromatography; mass spectrometry; medicinal plants; metabolites; metabolomics; methyl jasmonate; multivariate analysis; terpenoids; vinblastine; vincristine
Abstract:
... The medicinal plant Catharanthus roseus accumulates large numbers of terpenoid indole alkaloids (TIAs), including the pharmaceutically important vinblastine, vincristine, ajmalicine, and serpentine. The phytohormone ethylene or methyl jasmonate (MeJA) can markedly enhance alkaloid accumulation. The interaction between ethylene or MeJA in the regulation of TIA biosynthesis in C. roseus is unknown. ...
DOI:
10.1021/acs.jnatprod.7b00782

46. Naphthomycins L–N, Ansamycin Antibiotics from Streptomyces sp. CS

Author:
Yang Yin-He; Fu Xiao-Li; Li Liang-Qun; Zeng Ying; Li Cheng-Yun; He Yi-Neng; Zhao Pei-Ji
Source:
Journal of natural products 2012 v.75 no.7 pp. 1409-1413
ISSN:
1520-6025
Subject:
Streptomyces; antibiotics; liquid chromatography; mass spectrometry; multigene family; new products; nuclear magnetic resonance spectroscopy; oatmeal; screening; spectral analysis
Abstract:
... Previous analyses of the naphthomycin biosynthetic gene cluster and a comparison with known naphthomycin-type products from Streptomyces sp. CS have suggested that new products can be found from this strain. In this study, screening by LC-MS of Streptomyces sp. CS products formed under different culture conditions revealed several unknown peaks in the product spectra of extracts derived from oatme ...
DOI:
10.1021/np300109s

47. Nontargeted Metabolomics Approach for Age Differentiation and Structure Interpretation of Age-Dependent Key Constituents in Hairy Roots of Panax ginseng

Author:
Kim Nahyun; Kim Kemok; Lee DongHyuk; Shin Yoo-Soo; Bang Kyong-Hwan; Cha Seon-Woo; Lee Jae Won; Choi Hyung-Kyoon; Hwang Bang Yeon; Lee Dongho
Source:
Journal of natural products 2012 v.75 no.10 pp. 1777-1784
ISSN:
1520-6025
Subject:
Panax ginseng; biomarkers; fine roots; liquid chromatography; metabolites; metabolomics; quality control; tandem mass spectrometry
Abstract:
... The age of the ginseng plant has been considered as an important criterion to determine the quality of this species. For age differentiation and structure interpretation of age-dependent key constituents of Panax ginseng, hairy root (fine root) extracts aged from four to six years were analyzed using a nontargeted approach with ultraperformance liquid chromatography/quadrupole time-of-flight mass ...
DOI:
10.1021/np300499p

48. Occurrence of Mycotoxins in Botanical Dietary Supplement Infusion Beverages

Author:
Noelia Pallarés; Josefa Tolosa; Jordi Mañes; Emilia Ferrer
Source:
Journal of natural products 2019 v.82 no.2 pp. 403-406
ISSN:
1520-6025
Subject:
15-acetyldeoxynivalenol; Mentha pulegium; aflatoxins; anise; chamomile; dietary supplements; herbal tea; honey; liquid chromatography; liquid-phase microextraction; mint; nivalenol; ochratoxin A; risk assessment; tandem mass spectrometry; tea (beverage); thyme; zearalenone
Abstract:
... The aim of the present work was to study the occurrence of mycotoxins [aflatoxins (1–4), 3-acetyldeoxyniavlenol (5), 15-acetyldeoxynivalenol (6), nivalenol (7), HT-2 (8), T-2 (9), ochratoxin A (10), zearalenone (11), enniatin A (12), enniatin A1 (13), enniatin B (14), enniatin B1 (15), and beauvericin (16)] present in potable products derived from herbal teas. Analysis was carried out by liquid ch ...
DOI:
10.1021/acs.jnatprod.8b00283

49. Online Structural Elucidation of Alkaloids and Other Constituents in Crude Extracts and Cultured Cells of Nandina domestica by Combination of LC-MS/MS, LC-NMR, and LC-CD Analyses

Author:
Iwasa, Kinuko; Takahashi, Teturo; Nishiyama, Yumi; Moriyasu, Masataka; Sugiura, Makiko; Takeuchi, Atsuko; Tode, Chisato; Tokuda, Harukuni; Takeda, Kazuyoshi
Source:
Journal of natural products 2008 v.71 no.8 pp. 1376-1385
ISSN:
0163-3864
Subject:
Nandina domestica; medicinal plants; chemical constituents of plants; alkaloids; liquid chromatography; mass spectrometry; nuclear magnetic resonance spectroscopy; chemical structure; spectral analysis
DOI:
10.1021/np8001496
PubMed:
18671433

50. Pentaminomycins A and B, Hydroxyarginine-Containing Cyclic Pentapeptides from Streptomyces sp. RK88-1441

Author:
Jun-Pil Jang; Gwi Ja Hwang; Min Cheol Kwon; In-Ja Ryoo; Mina Jang; Shunji Takahashi; Sung-Kyun Ko; Hiroyuki Osada; Jae-Hyuk Jang; Jong Seog Ahn
Source:
Journal of natural products 2018 v.81 no.4 pp. 806-810
ISSN:
1520-6025
Subject:
Streptomyces; amino acids; cyclic peptides; enzymes; hydrolysates; liquid chromatography; mass spectrometry; melanin; nuclear magnetic resonance spectroscopy
Abstract:
... Two new cyclic peptides, pentaminomycins A (1) and B (2), were isolated from cultures of Streptomyces sp. RK88-1441. Based on the interpretation of the NMR, UV, IR, and MS data, the planar structures of 1 and 2 were elucidated as cyclic pentapeptides with a modified amino acid residue, N⁵-hydroxyarginine (N5-OH-Arg). The absolute configurations of the constituent amino acid residues were determine ...
DOI:
10.1021/acs.jnatprod.7b00882