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- Sau, Sujay
P.; Hrdlicka, Patrick J.
- Journal of organic chemistry 2012 v.77 no.1 pp. 5-16
- DNA; DNA primers; RNA; absorption; biotechnology; chemical reactions; denaturation; fluorescence emission spectroscopy; hybridization; hydrogen bonding; microarray technology; moieties; nucleic acid hybridization; organic chemistry; organic compounds; polymerase chain reaction; temperature; thermal stability
- ... Development of universal hybridization probes, that is, oligonucleotides displaying identical affinity toward matched and mismatched DNA/RNA targets, has been a longstanding goal due to potential applications as degenerate PCR primers and microarray probes. The classic approach toward this end has been the use of “universal bases” that either are based on hydrogen-bonding purine derivatives or aro ...
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