Journal: Journal of proteomics / Publication Year: 2019 / Source: 2019 v.192 / Subject: biomass

Main content area

Author:: Türkowsky, Dominique; Lohmann, Patrick; Mühlenbrink, Marie; Schubert, Torsten; Adrian, Lorenz; Goris, Tobias; Jehmlich, Nico; von Bergen, Martin
Source:: Journal of proteomics 2019 v.192 pp. 10-17
ISSN:: 1874-3919
Subject:: Sulfurospirillum; anaerobes; anaerobic conditions; bacteria; biomass; dehalogenation; enzyme substrates; eukaryotic cells; ligands; mass spectrometry; melting; oxidoreductases; proteins; proteome; protocols; substrate specificity; temperature; tetrachloroethylene; thermal stability
Abstract:: ... Thermal proteome profiling (TPP) is increasingly applied in eukaryotes to investigate protein-ligand binding through protein melting curve shifts induced by the presence of a ligand. In anaerobic bacteria, identification of protein-substrate interactions is a major challenge. We applied TPP to Sulfurospirillum multivorans, which is able to use trichloroethene as electron acceptor for growth, to in ...
DOI:: 10.1016/j.jprot.2018.05.018
: https://dx.doi.org/10.1016/j.jprot.2018.05.018