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You searched for: Journal Journal of proteomics Remove constraint Journal: Journal of proteomics Publication Year 2019 Remove constraint Publication Year: 2019 Subject biomass Remove constraint Subject: biomass
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1. Manganese alters expression of proteins involved in the oxidative stress of Meyerozyma guilliermondii

Author:
Ruas, France Anne Dias; Barboza, Natália Rocha; Castro-Borges, William; Guerra-Sá, Renata
Source:
Journal of proteomics 2019 v.196 pp. 173-188
ISSN:
1874-3919
Subject:
DNA repair; Meyerozyma guilliermondii; bacteria; biomass; bioremediation; byproducts; drainage; enzyme activity; gene expression regulation; haploidy; heavy metals; hyperaccumulators; liquid chromatography; manganese; mining; oxidative stress; peptides; pollution; protein composition; protein synthesis; proteins; proteome; proteomics; tandem mass spectrometry; yeasts; Brazil
Abstract:
... Organisms, in general, respond to environmental stress by altering their pattern of protein expression (proteome), as an alternative to growing in stressful conditions. A strain of Meyerozyma guilliermondii resistant to manganese was isolated from a sample of water collected from mine drainage in southeastern Minas Gerais (Brazil), and demonstrated manganese detoxification capacity. Protein extrac ...
DOI:
10.1016/j.jprot.2018.11.001

2. Thermal proteome profiling allows quantitative assessment of interactions between tetrachloroethene reductive dehalogenase and trichloroethene

Author:
Türkowsky, Dominique; Lohmann, Patrick; Mühlenbrink, Marie; Schubert, Torsten; Adrian, Lorenz; Goris, Tobias; Jehmlich, Nico; von Bergen, Martin
Source:
Journal of proteomics 2019 v.192 pp. 10-17
ISSN:
1874-3919
Subject:
Sulfurospirillum; anaerobes; anaerobic conditions; bacteria; biomass; dehalogenation; enzyme substrates; eukaryotic cells; ligands; mass spectrometry; melting; oxidoreductases; proteins; proteome; protocols; substrate specificity; temperature; tetrachloroethylene; thermal stability
Abstract:
... Thermal proteome profiling (TPP) is increasingly applied in eukaryotes to investigate protein-ligand binding through protein melting curve shifts induced by the presence of a ligand. In anaerobic bacteria, identification of protein-substrate interactions is a major challenge. We applied TPP to Sulfurospirillum multivorans, which is able to use trichloroethene as electron acceptor for growth, to in ...
DOI:
10.1016/j.jprot.2018.05.018