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- Türkowsky, Dominique; Lohmann, Patrick; Mühlenbrink, Marie; Schubert, Torsten; Adrian, Lorenz; Goris, Tobias; Jehmlich, Nico; von Bergen, Martin
- Journal of proteomics 2019 v.192 pp. 10-17
- Sulfurospirillum; anaerobes; anaerobic conditions; bacteria; biomass; dehalogenation; enzyme substrates; eukaryotic cells; ligands; mass spectrometry; melting; oxidoreductases; proteins; proteome; protocols; substrate specificity; temperature; tetrachloroethylene; thermal stability
- ... Thermal proteome profiling (TPP) is increasingly applied in eukaryotes to investigate protein-ligand binding through protein melting curve shifts induced by the presence of a ligand. In anaerobic bacteria, identification of protein-substrate interactions is a major challenge. We applied TPP to Sulfurospirillum multivorans, which is able to use trichloroethene as electron acceptor for growth, to in ...