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You searched for: Journal name Journal of proteomics Remove constraint Journal name: Journal of proteomics Publication year rev 7982-2018 Remove constraint Publication year rev: 7982-2018 Source 2018 Remove constraint Source: 2018
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1. Combined proteomic and functional analysis reveals rich sources of protein diversity in skin mucus and venom from the Scorpaena plumieri fish

Author:
Márcia H. Borges; Filipe Andrich; Pedro H. Lemos; Thiago G. Soares; Thiago N. Menezes; Fabiana V. Campos; Leandro X. Neves; William Castro-Borges; Suely G. Figueiredo
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
Scorpaena plumieri; antimicrobial properties; bioactive compounds; fish; hyaluronoglucosaminidase; lectins; mucus; protein composition; proteinase inhibitors; proteinases; proteolysis; proteomics; toxicity; toxins; venoms
Abstract:
... The biological activities observed upon envenomation by Scorpaena plumieri could be linked to both the venom and the skin mucus. Through a proteomic/functional approach we analyzed protein composition and biological activities of the venom and skin mucus. We identified 885 proteins: 722 in the Venomous Apparatus extracts (Sp-VAe) and 391 in the Skin Mucus extract (Sp-SMe), with 494 found exclusive ...
DOI:
10.1016/j.jprot.2018.08.002

2. Comparative proteomics of respiratory exosomes in cystic fibrosis, primary ciliary dyskinesia and asthma

Author:
Virginie Rollet-Cohen; Matthieu Bourderioux; Joanna Lipecka; Cerina Chhuon; Vincent A. Jung; Myriam Mesbahi; Thao Nguyen-Khoa; Sophie Guérin-Pfyffer; Alain Schmitt; Aleksander Edelman; Isabelle Sermet-Gaudelus; Ida Chiara Guerrera
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
asthma; chemotaxis; cystic fibrosis; dyskinesia; electron microscopy; exosomes; glutathione; inflammation; leukocytes; lungs; mass spectrometry; patients; proteomics; superoxide dismutase; transcription factor NF-kappa B
Abstract:
... Cystic fibrosis (CF) and primary ciliary dyskinesia (PCD) are pulmonary genetic disorders associated with inflammation and heterogeneous progression of the lung disease. We hypothesized that respiratory exosomes, nanovesicles circulating in the respiratory tract, may be involved in the progression of inflammation-related lung damage. We compared proteomic content of respiratory exosomes isolated f ...
DOI:
10.1016/j.jprot.2018.07.001

3. Compositional and functional investigation of individual and pooled venoms from long-term captive and recently wild-caught Bothrops jararaca snakes

Author:
Nathália da Costa Galizio; Caroline Serino-Silva; Daniel Rodrigues Stuginski; Patrícia Antônia Estima Abreu; Sávio Stefanini Sant'Anna; Kathleen Fernandes Grego; Alexandre Keiji Tashima; Anita Mitico Tanaka-Azevedo; Karen de Morais-Zani
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
Bothrops jararaca; antivenoms; coagulants; enzyme activity; intraspecific variation; protein composition; proteolysis; proteomics; reversed-phase high performance liquid chromatography; snake bites; snake venoms; snakes; toxins; Brazil
Abstract:
... Intraspecific venom variability has been extensively reported in a number of species and is documented to be the result of several factors. However, current evidence for snake venom variability related to captivity maintenance is controversial. Here we report a compositional and functional investigation of individual and pooled venoms from long-term captive (LTC) and recently wild-caught (RWC) B. ...
DOI:
10.1016/j.jprot.2018.07.007

4. Differential protein expression of hippocampal cells associated with heavy metals (Pb, As, and MeHg) neurotoxicity: Deepening into the molecular mechanism of neurodegenerative diseases

Author:
Venkatanaidu Karri; David Ramos; Julia Bauzá Martinez; Antonia Odena; Eliandre Oliveira; Susan L. Coort; Chris T. Evelo; Edwin C.M. Mariman; Marta Schuhmacher; Vikas Kumar
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
Alzheimer disease; Parkinson disease; arsenic; bioassays; biomarkers; brain; chronic exposure; epidemiological studies; filters; gene ontology; genes; heavy metals; humans; lead; messenger RNA; methylmercury compounds; mitochondria; models; neurotoxicity; oxidative stress; protein content; protein synthesis; proteome; proteomics; risk; risk assessment; toxicity testing; ubiquitin
Abstract:
... Chronic exposure to heavy metals such as Pb, As, and MeHg can be associated with an increased risk of developing neurodegenerative diseases. Our in vitro bioassays results showed the potency of heavy metals in the order of Pb < As < MeHg on hippocampal cells. The main objective of this study was combining in vitro label free proteomics and systems biology approach for elucidating patterns of biolo ...
DOI:
10.1016/j.jprot.2018.06.020

5. Discrimination and quantification of homologous keratins from goat and sheep with dual protease digestion and PRM assays

Author:
Chen Miao; Yunfei Yang; Shanshan Li; Yufeng Guo; Wenqing Shui; Qichen Cao
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
amino acid sequences; databases; enzymatic hydrolysis; fabrics; goats; humans; keratin; mass spectrometry; monitoring; peptides; proteome; proteomics; sequence analysis; sheep; trypsin; wool
Abstract:
... Mass spectrometry (MS) technology has a special advantage in species determination for protein-rich samples which requires identification of species-specific peptides. However, for species discrimination of highly homologous proteins, it remains challenging to select the species unique peptides with routine proteomics approaches. In this work, we chose keratins and keratin-associated proteins (KAP ...
DOI:
10.1016/j.jprot.2018.07.010

6. Effects of manganese toxicity on the protein profile of tomato (Solanum lycopersicum) roots as revealed by two complementary proteomic approaches, two-dimensional electrophoresis and shotgun analysis

Author:
Laura Ceballos-Laita; Elain Gutierrez-Carbonell; Hiroyuki Imai; Anunciación Abadía; Matsuo Uemura; Javier Abadía; Ana Flor López-Millán
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
Solanum lycopersicum; amino acid metabolism; cell walls; defense mechanisms; energy; glycolysis; hydrolases; lignification; manganese; oxidative phosphorylation; pathogenesis-related proteins; peroxidases; protein composition; proteome; proteomics; pyruvic acid; roots; suberization; tomatoes; toxicity; two-dimensional gel electrophoresis
Abstract:
... The aim of this work was to assess the effects of manganese (Mn) toxicity on the proteome of tomato roots using two proteomic approaches, shotgun and two-dimensional electrophoresis. The shotgun approach yielded 367 reliable proteins, whereas the 2-DE approach detected 340 consistent spots. The 2-DE method found 54 proteins changing in relative abundance in the excess Mn treatment, whereas the sho ...
DOI:
10.1016/j.jprot.2018.06.016

7. Exosomes derived from calcium oxalate-exposed macrophages enhance IL-8 production from renal cells, neutrophil migration and crystal invasion through extracellular matrix

Author:
Nilubon Singhto; Visith Thongboonkerd
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
actin; binding capacity; calcium; calcium oxalate; crystals; cytoskeleton; exosomes; extracellular matrix; immune response; inflammation; interleukin-8; kidney cells; macrophages; neutrophils; proteomics; renal calculi; stress response; tandem mass spectrometry; transcription (genetics)
Abstract:
... Deposition of calcium oxalate (CaOx) crystals in renal interstitium is one of the key factors that cause progressive inflammation in kidney stone disease. Macrophages are responsible for elimination of these crystals but their roles to worsen inflammatory process remain under-investigated. This study thus aimed to define roles of exosomes released from macrophages exposed to CaOx crystals in media ...
DOI:
10.1016/j.jprot.2018.06.015

8. Insight into the biological pathways underlying fibromyalgia by a proteomic approach

Author:
Jorge A. Ramírez-Tejero; Esther Martínez-Lara; Alma Rus; María Victoria Camacho; María Luisa Del Moral; Eva Siles
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
coagulation; complement; erythrocyte sedimentation rate; etiology; fibrinogen; gene expression regulation; genes; haptoglobins; inflammation; liquid chromatography; pathogenesis; patients; protein composition; proteome; proteomics; rheumatoid arthritis; tandem mass spectrometry
Abstract:
... Fibromyalgia (FM) is a form of non-articular rheumatism difficult to diagnose and treat because its etiology remains still elusive. Proteomics makes possible the systematic analysis of hundreds of proteins in clinical samples. Consequently, it has become a key tool for finding altered molecular pathways in different diseases. In this context, the present study analyzes changes in the plasma proteo ...
DOI:
10.1016/j.jprot.2018.07.009

9. Label-free quantitative proteomics of rat hypothalamus under fever induced by LPS and PGE2

Author:
Marina Firmino; Simone N. Weis; Jaques M.F. Souza; Bruna R.B. Gomes; Alan R. Mól; Márcia R. Mortari; Gloria E.P. Souza; Guilherme C. Coca; Thomas C.R. Williams; Wagner Fontes; Carlos André O. Ricart; Marcelo V. de Sousa; Fabiane H. Veiga-Souza
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
antioxidants; data collection; fever; glycolysis; heat; hypothalamus; inflammation; lipopolysaccharides; liquid chromatography; mitochondria; models; oxidative stress; pentose phosphate cycle; prostaglandins; proteins; proteome; proteomics; rats; tandem mass spectrometry; tricarboxylic acid cycle
Abstract:
... Fever is a brain-mediated increase in body temperature mainly during inflammatory or infectious challenges. Although there is considerable data regarding the inflammation pathways involved in fever, metabolic alterations necessary to orchestrate the complex inflammatory response are not totally understood. We performed proteomic analysis of rat hypothalamus using label-free LC–MS/MS in a model of ...
DOI:
10.1016/j.jprot.2018.07.018

10. Proteomic profile, biological activities and antigenic analysis of the venom from Bothriopsis bilineata smaragdina (“loro machaco”), a pitviper snake from Peru

Author:
Carolina Rego Rodrigues; Frey Francisco Romero Vargas; Clara Guerra-Duarte; Fernanda Costal-Oliveira; Stephanie Stransky Láuar; Letícia Lopes de Souza; Alexandre Augusto Assis Dutra; André Teixeira-Ferreira; Armando Yarlequé; César Bonilla; Eladio Flores Sanchez; Jonas Perales; Carlos Chávez-Olórtegui
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
Bothrops; Western blotting; accidents; antivenoms; cell viability; edema; enzyme activity; enzyme-linked immunosorbent assay; hemorrhage; humans; lectins; lethal dose 50; metalloproteinases; mice; neutralization; phospholipases; polyacrylamide gel electrophoresis; proteomics; public health; rain forests; reversed-phase high performance liquid chromatography; serine proteinases; snake bites; snake venoms; snakes; sympatry; Amazonia; Brazil; Latin America; Peru
Abstract:
... In order to determine Bothriopsis bilineata smaragdina venom (BbsV) composition, proteomic approaches were performed. Venom components were analyzed by RP-HPLC, SDS- PAGE and nano LC on line with LTQ Orbitrap XL. Results showed a total of 189 identified proteins, grouped into 11 different subgroups, which include snake venom metalloproteinases (SVMPs, 54.67%), snake C-type lectins (Snaclecs, 15.78 ...
DOI:
10.1016/j.jprot.2018.07.016

11. Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection

Author:
Dan Zhang; Xinyue Sun; Liqin Ren; Chunyan Yang; Xianbing Liu; Haixia Zhang; Yuzhu Jiang; Xuemei Hu
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
Toxoplasma gondii; Western blotting; abortion (animals); congenital abnormalities; fetal development; humans; immune response; immune system; immunosuppression; pregnancy outcome; protein synthesis; proteins; proteome; proteomics; quantitative polymerase chain reaction; tissues; trophoblast
Abstract:
... A Toxoplasma gondii infection during pregnancy can result in spontaneous abortion, preterm labor, or congenital fetal defects. The decidual immune system plays a critical role in regulating the immune micro-environment and in the induction of immune tolerance. To better understand the factors that mediate the decidual immune response associated with the T. gondii infection, a large-scale study emp ...
DOI:
10.1016/j.jprot.2018.07.011

12. Quality control of single amino acid variations detected by tandem mass spectrometry

Author:
Xinpei Yi; Bo Wang; Zhiwu An; Fuzhou Gong; Jing Li; Yan Fu
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
amino acids; cell lines; colorectal neoplasms; computer software; control methods; data collection; genome; genotype; neoplasm cells; peptides; phenotype; proteins; proteomics; quality control; sequence analysis; single nucleotide polymorphism; tandem mass spectrometry
Abstract:
... Study of single amino acid variations (SAVs) of proteins, resulting from single nucleotide polymorphisms, is of great importance for understanding the relationships between genotype and phenotype. In mass spectrometry based shotgun proteomics, identification of peptides with SAVs often suffers from high error rates on the variant sites detected. These site errors are due to multiple reasons and ca ...
DOI:
10.1016/j.jprot.2018.07.004

13. Quantitative proteomics of psychotrophic diazotroph in response to nitrogen deficiency and cold stress

Author:
Deep Chandra Suyal; Saurabh Kumar; Divya Joshi; Ravindra Soni; Reeta Goel
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
Pseudomonas; agricultural management; biomarkers; cold stress; databases; environmental sustainability; gels; gene expression regulation; liquid chromatography; monitoring; niches; nitrogen; nitrogen fixation; nitrogen-fixing bacteria; nitrogenase; plant protection; proteins; proteome; proteomics; protocols; tandem mass spectrometry
Abstract:
... Effective protocols and novel biomarkers are the need of this hour to screen potential cold adapted diazotrophs for sustainable mountain agricultural plans. LC-MS/MS based gel less quantitative proteomics was employed to investigate the metabolic response of Himalayan cold adapted diazotroph Pseudomonas palleroniana N26 (JN055435) for nitrogen deficiency and cold stress. More than 5000 proteins we ...
DOI:
10.1016/j.jprot.2018.08.005

14. Quantitative proteomics reveals proteins involved in the progression from non-cancerous lesions to gastric cancer

Author:
Diana Lashidua Fernández-Coto; Jeovanis Gil; Angélica Hernández; Roberto Herrera-Goepfert; Ivone Castro-Romero; Eva Hernández-Márquez; Ana Silvia Arenas-Linares; Valia Tletzalli Calderon-Sosa; Miguel Ángel Sanchez-Aleman; Alfonso Mendez-Tenorio; Sergio Encarnación-Guevara; Guadalupe Ayala
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
DNA replication; adenocarcinoma; biomarkers; biopsy; cell viability; enzymes; gastritis; gene expression regulation; gene overexpression; glycolysis; intestines; mass spectrometry; metaplasia; mitochondria; mortality; phenotype; proteomics; ribosomal proteins; spliceosomes; stomach neoplasms
Abstract:
... Gastric cancer is one of the most aggressive malignancies affecting humankind. With almost a million cases globally, it sits in fifth position in terms of incidence, and third in terms of mortality. The progression of this disease is slow, with prolonged and sequential precancerous stages including chronic gastritis, intestinal metaplasia, dysplasia, and finally gastric cancer. Here we used the iT ...
DOI:
10.1016/j.jprot.2018.07.013

15. Serum responsive proteome reveals correlation between oxidative phosphorylation and morphogenesis in Candida albicans ATCC10231

Author:
Radfan Ahmed; Santosh Kodgire; B. Santhakumari; Rajendra Patil; Mahesh Kulkarni; Gajanan Zore
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
Candida albicans; amino acids; chitin; ergosterol; fatty acid metabolism; fetal bovine serum; glycolysis; hyphae; liquid chromatography; mitogen-activated protein kinase; morphogenesis; oxidative phosphorylation; proteins; proteome; renewable energy sources; sodium azide; tandem mass spectrometry; tricarboxylic acid cycle
Abstract:
... To understand the impact of fetal bovine serum (FBS) on metabolism and cellular architecture in addition to morphogenesis, we have identified FBS responsive proteome of Candida albicans. FBS induced 34% hyphae and 60% pseudohyphae in C. albicans at 30 °C while 98% hyphae at 37 °C. LC-MS/MS analysis revealed that 285 proteins modulated significantly in response to FBS at 30 °C and 37 °C. Out of whi ...
DOI:
10.1016/j.jprot.2018.06.018

16. A large-scale and robust dynamic MRM study of colorectal cancer biomarkers

Author:
Jia You; Athit Kao; Roslyn Dillon; Lisa J. Croner; Ryan Benz; John E. Blume; Bruce Wilcox
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
biomarkers; blood sampling; colorectal neoplasms; data collection; experimental design; mass spectrometry; monitoring; patients; peptides; process control; proteins; proteomics; risk
Abstract:
... Over the past 20 years, mass spectrometry (MS) has emerged as a dynamic tool for proteomics biomarker discovery. However, published MS biomarker candidates often do not translate to the clinic, failing during attempts at independent replication. The cause can be shortcomings in study design, sample quality, assay quantitation, and/or quality/process control. To address these shortcomings, we devel ...
DOI:
10.1016/j.jprot.2018.06.013

17. Early changes in the urine proteome in a diethyldithiocarbamate-induced chronic pancreatitis rat model

Author:
Linpei Zhang; Yuqiu Li; Youhe Gao
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
animal models; biomarkers; blood serum; chronic diseases; cytokines; early diagnosis; extracellular matrix; fibrosis; gene expression regulation; homeostasis; humans; intraperitoneal injection; liquid chromatography; pancreatitis; patients; proteins; proteome; rats; tandem mass spectrometry; urine
Abstract:
... Without homeostasis mechanisms, urine accumulates early changes in biomarkers and can be a better and earlier biomarker source than blood, especially for chronic diseases. This study tests whether early changes can be detected in a rat model of chronic pancreatitis induced by intraperitoneal injection of diethyldithiocarbamate. Urinary proteins from three rats were profiled by liquid chromatograph ...
DOI:
10.1016/j.jprot.2018.07.015

18. Embryonic atrazine exposure elicits proteomic, behavioral, and brain abnormalities with developmental time specific gene expression signatures

Author:
Katharine A. Horzmann; Leeah S. Reidenbach; Devang H. Thanki; Anna E. Winchester; Brad A. Qualizza; Geoffrey A. Ryan; Kaitlyn E. Egan; Victoria E. Hedrick; Tiago J.P. Sobreira; Samuel M. Peterson; Gregory J. Weber; Sara E. Wirbisky-Hershberger; Maria S. Sepúlveda; Jennifer L. Freeman
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
Danio rerio; atrazine; brain; carbon-sulfur lyases; cytochrome P-450; developmental toxicity; embryogenesis; endocrine-disrupting chemicals; gene expression; gene expression regulation; larvae; neoplasms; neurodevelopment; protein-serine-threonine kinases; proteomics; transcriptomics; water pollution; United States
Abstract:
... Atrazine (ATZ), the second most commonly used herbicide in the United States, is an endocrine disrupting chemical linked to cancer and a common drinking water contaminant. This study further investigates ATZ-related developmental toxicity by testing the following hypotheses in zebrafish: the effects of embryonic ATZ exposure are dependent on timing of exposure; embryonic ATZ exposure alters brain ...
DOI:
10.1016/j.jprot.2018.07.006
PubMed:
30012420
PubMed Central:
PMC6193558

19. Long-term moderate exercise enhances specific proteins that constitute neurotrophin signaling pathway: A TMT-based quantitative proteomic analysis of rat plasma

Author:
Yuan Wei; Chun-Lu Fang; Shu-Jing Liu; Wen-Qi Yang; Li-Shen Wei; Xiao-Juan Lei; Fei Hu; Hao-Ying Huang; Wei Li; Wei Chen; Liang-Ming Li; Yue-Sheng Long
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
Western blotting; apoptosis; bioinformatics; biomarkers; blood plasma; blood proteins; cell viability; diabetes; energy metabolism; exercise; hippocampus; homeostasis; immunocompetence; longevity; neoplasms; nervous system diseases; obesity; plasticity; protein composition; protein-protein interactions; proteomics; rats; risk; signal transduction; stroke; synapse; tissues
Abstract:
... Physical exercise has been reported to increase neurotrophin in brain tissues as hippocampus as well as increased neurotrophic level peripherally in blood plasma and might have an effect on/or affect molecular processes of energy metabolism (and homeostasis). In this study, using quantitative proteomic analysis, we obtained a plasma protein profile from the rat with long-term moderate exercise. A ...
DOI:
10.1016/j.jprot.2018.06.017

20. Lysine acetylproteome profiling under water deficit reveals key acetylated proteins involved in wheat grain development and starch biosynthesis

Author:
Geng-Rui Zhu; Xing Yan; Dong Zhu; Xiong Deng; Ji-Su Wu; Jian Xia; Yue-Ming Yan
Source:
Journal of proteomics 2018 pp. -
ISSN:
1874-3919
Subject:
Western blotting; acetylation; biochemical pathways; biosynthesis; drought; lysine; phosphorylation; post-translational modification; proteins; proteome; quantitative polymerase chain reaction; seed development; starch; sucrose synthase; tandem mass spectrometry; transcription (genetics); wheat
Abstract:
... Lysine acetylation is a widespread protein posttranslational modification in all organisms. However, quantitative acetylproteome characterization in response to water deficit during crop grain development remains unknown. In the study, we performed the first large-scale acetylproteome analysis of developing wheat grains under water-deficit using label-free quantitative proteome approach. In total, ...
DOI:
10.1016/j.jprot.2018.06.019