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You searched for: Journal Journal of proteomics Remove constraint Journal: Journal of proteomics Publication Year 2017 Remove constraint Publication Year: 2017 Source 2017 v.164 Remove constraint Source: 2017 v.164
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1. Absolute venomics: Absolute quantification of intact venom proteins through elemental mass spectrometry

Author:
Calderón-Celis, Francisco; Cid-Barrio, Laura; Encinar, Jorge Ruiz; Sanz-Medel, Alfredo; Calvete, Juan J.
Source:
Journal of proteomics 2017 v.164 pp. 33-42
ISSN:
1874-3919
Subject:
Naja; Pseudechis; antivenoms; data collection; evolution; humans; hybrids; isotope dilution technique; mass spectrometry; models; predators; proteins; proteome; proteomics; public health; rural communities; snake bites; snakes; toxicity; toxins; transcriptomics; venoms; New Guinea; Tanzania
Abstract:
... We report the application of a hybrid element and molecular MS configuration for the parallel absolute quantification of μHPLC-separated intact sulfur-containing venom proteins, via ICP triple quadrupole MS and ³²S/³⁴S isotope dilution analysis, and identification by ESI-QToF-MS of the toxins of the medically important African black-necked spitting cobra, Naja nigricollis (Tanzania); New Guinea sm ...
DOI:
10.1016/j.jprot.2017.06.001

2. Determination of the protein expression profiles of Propsilocerus akamusi (Tokunaga) Malpighian tubules response to cadmium stress by iTRAQ coupled LC–MS/MS

Author:
Zheng, Xianyun; Xie, Zhiying; Wang, Shu; Lin, Pipeng
Source:
Journal of proteomics 2017 v.164 pp. 85-93
ISSN:
1874-3919
Subject:
Chironomidae; Malpighian tubules; aquatic food webs; cadmium; ecosystems; fish; food chain; insects; lipids; prey species; protein synthesis; proteins; proteome; proteomics; water birds
Abstract:
... Propsilocerus akamusi (Tokunaga) is an important prey species for fish and aquatic birds, which represent an important link in the aquatic food chain as other species of Chironomidae. Malpighian tubules (MT) play an important role in metabolism and detoxification of harmful compounds and metal accumulation. Our previous studies have reported that P. akamusi have an extraordinary resistance to Cd. ...
DOI:
10.1016/j.jprot.2017.05.017

3. Hydrocarbon-induced changes in proteins and fatty acids profiles of Raoultella ornithinolytica M03

Author:
Zdarta, A.; Tracz, J.; Luczak, M.; Guzik, U.; Kaczorek, E.
Source:
Journal of proteomics 2017 v.164 pp. 43-51
ISSN:
1874-3919
Subject:
Raoultella ornithinolytica; benzene derivatives; biodegradation; biogenesis; biosynthesis; cell membranes; fatty acid composition; fatty acids; gluconeogenesis; hydrophobicity; ligases; membrane permeability; microorganisms; protein composition; protein synthesis; proteins; remediation; transfer RNA; tricarboxylic acid cycle
Abstract:
... Microorganisms can support environmental restoration by biodegradation of hydrocarbons but the mechanism of this process has been not described in detail yet. We present the effect of benzene derivatives on Raoultella ornithinolytica M03 cell composition. Comparison of the cell response after short-term and long-term stress revealed significant differences in surface properties, fatty acid composi ...
DOI:
10.1016/j.jprot.2017.05.028

4. Isolation and characterization of Conohyal-P1, a hyaluronidase from the injected venom of Conus purpurascens

Author:
Mӧller, Carolina; Clark, Evan; Safavi-Hemami, Helena; DeCaprio, Anthony; Marí, Frank
Source:
Journal of proteomics 2017 v.164 pp. 73-84
ISSN:
1874-3919
Subject:
Conus; animal tissues; asparagine; clinical trials; conotoxins; drugs; extracellular matrix; glycosaminoglycans; glycosylation; hyaluronoglucosaminidase; mass spectrometry; proteins; proteomics; snails; transcriptomics
Abstract:
... Hyaluronidases are ubiquitous enzymes commonly found in venom and their main function is to degrade hyaluran, which is the major glycosaminoglycan of the extracellular matrix in animal tissues. Here we describe the purification and characterization of a 60kDa hyaluronidase found in the injected venom from Conus purpurascens, Conohyal-P1. Using a combined strategy based on transcriptomic and proteo ...
DOI:
10.1016/j.jprot.2017.05.002

5. Proteomic fingerprinting of mistletoe (Viscum album L.) via combinatorial peptide ligand libraries and mass spectrometry analysis

Author:
Vergara-Barberán, María; Lerma-García, María Jesús; Nicoletti, Maria; Simó-Alfonso, Ernesto Francisco; Herrero-Martínez, José Manuel; Fasoli, Elisa; Righetti, Pier Giorgio
Source:
Journal of proteomics 2017 v.164 pp. 52-58
ISSN:
1874-3919
Subject:
Viscum album; antineoplastic activity; databases; human health; immune system; ligands; mass spectrometry; medicine; neoplasm cells; neoplasms; pH; patients; proteins; proteome; proteomics; quality of life; therapeutics
Abstract:
... Combinatorial peptide ligand libraries (CPLLs), coupled to mass spectrometry (MS) analysis, have been used to investigate in depth the proteome of Viscum album L. (VA), commonly named European mistletoe, in order to provide a first proteomic fingerprinting. For this purpose, the proteins were captured via CPLLs at two different pH values (acidic and neutral). A total of 648 non-redundant proteins ...
DOI:
10.1016/j.jprot.2017.05.025

6. Proteomics analysis of Fusarium proliferatum under various initial pH during fumonisin production

Author:
Li, Taotao; Gong, Liang; Wang, Yong; Chen, Feng; Gupta, Vijai Kumar; Jian, Qijie; Duan, Xuewu; Jiang, Yueming
Source:
Journal of proteomics 2017 v.164 pp. 59-72
ISSN:
1874-3919
Subject:
Fusarium proliferatum; L-amino-acid oxidase; animal and human health; biosynthesis; citrates; cytochrome P-450; fumonisins; fungi; isocitrate dehydrogenase; methionine adenosyltransferase; mycelium; pH; pathogens; polyacrylamide gel electrophoresis; polyketide synthases; proteomics
Abstract:
... Fusarium proliferatum as a fungal pathogen can produce fumonisin which causes a great threat to animal and human health. Proteomic approach was a useful tool for investigation into mycotoxin biosynthesis in fungal pathogens. In this study, we analyzed the fumonisin content and mycelium proteins of Fusarium proliferatum cultivated under the initial pH5 and 10. Fumonisin production after 10days was ...
DOI:
10.1016/j.jprot.2017.05.008

7. Serine protease isoforms in Gloydius intermedius venom: Full sequences, molecular phylogeny and evolutionary implications

Author:
Yang, Zhang-Min; Yu, Hui; Liu, Zhen-Zhen; Pei, Jian-Zhu; Yang, Yu-E; Yan, Su-Xian; Zhang, Cui; Zhao, Wen-long; Wang, Zhe-Zhi; Wang, Ying-Ming; Tsai, Inn-Ho
Source:
Journal of proteomics 2017 v.164 pp. 19-32
ISSN:
1874-3919
Subject:
Gloydius; amino acid sequences; anion exchange; blood proteins; coagulation; complementary DNA; expressed sequence tags; glycosylation; high performance liquid chromatography; molecular cloning; neurotoxicity; phylogeny; plasminogen activator; quantitative polymerase chain reaction; sequence homology; structure-activity relationships; transcriptomics; venoms
Abstract:
... Nine distinct venom serine proteases (vSPs) of Gloydius intermedius were studied by transcriptomic, sub-proteomic and phylogenetic analyses. Their complete amino acid sequences were deduced after Expression Sequence Tag (EST) analyses followed by cDNA cloning and sequencing. These vSPs appear to be paralogs and contain the catalytic triads and 1–4 potential N-glycosylation sites. Their relative ex ...
DOI:
10.1016/j.jprot.2017.05.024

8. Venomics of Bungarus caeruleus (Indian krait): Comparable venom profiles, variable immunoreactivities among specimens from Sri Lanka, India and Pakistan

Author:
Oh, Angeline Mei Feng; Tan, Choo Hock; Ariaranee, Gnanathasan Christeine; Quraishi, Naeem; Tan, Nget Hong
Source:
Journal of proteomics 2017 v.164 pp. 1-18
ISSN:
1874-3919
Subject:
Bungarus; antivenoms; chromatography; cytotoxins; death; monitoring; neurotoxicity; neurotoxins; neutralization; pain; paralysis; pathophysiology; patients; phospholipases; protein composition; proteins; proteome; proteomics; snake bites; snakes; venoms; India; Pakistan; Sri Lanka
Abstract:
... The Indian krait (Bungarus caeruleus) is one of the “Big Four” venomous snakes widely distributed in South Asia. The present venomic study reveals that its venom (Sri Lankan origin) is predominated by phospholipases A2 (64.5% of total proteins), in which at least 4.6% are presynaptically-acting β-bungarotoxin A-chains. Three-finger toxins (19.0%) are the second most abundant, comprising 15.6% κ-ne ...
DOI:
10.1016/j.jprot.2017.04.018