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You searched for: Journal Journal of proteomics Remove constraint Journal: Journal of proteomics Publication Year 2015 Remove constraint Publication Year: 2015 Source 2015 v.113 Remove constraint Source: 2015 v.113
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1. Absolute quantification of Corynebacterium glutamicum glycolytic and anaplerotic enzymes by QconCAT

Author:
Voges, Raphael; Corsten, Stephanie; Wiechert, Wolfgang; Noack, Stephan
Source:
Journal of proteomics 2015 v.113 pp. 366-377
ISSN:
1874-3919
Subject:
Corynebacterium glutamicum; Saccharomyces cerevisiae; biotechnology; carbon; catalysts; essential amino acids; glycolysis; hosts; humans; lysine; phosphofructokinases; phosphopyruvate hydratase; prokaryotic cells; soil bacteria; yeasts
Abstract:
... The soil bacterium Corynebacterium glutamicum is one of the best-studied production hosts for industrial biotechnology, and it is primarily used for the large-scale production of essential amino acids, such as l-lysine. For rational strain development, detailed knowledge of intracellular protein concentration is crucial to determine metabolic capacities and limitations. We developed a QconCAT appr ...
DOI:
10.1016/j.jprot.2014.10.008

2. Analysis of epididymal sperm maturation by MALDI profiling and top-down mass spectrometry

Author:
Labas, Valérie; Spina, Lucie; Belleannee, Clémence; Teixeira-Gomes, Ana-Paula; Gargaros, Audrey; Dacheux, Françoise; Dacheux, Jean-Louis
Source:
Journal of proteomics 2015 v.113 pp. 226-243
ISSN:
1874-3919
Subject:
Sus scrofa; boars; enzyme activity; epididymis; matrix-assisted laser desorption-ionization mass spectrometry; peptides; phenotype; post-translational modification; proteins; proteolysis; spermatozoa
Abstract:
... The fertilization ability of male gametes is achieved after their transit through the epididymis where important post-gonadal differentiation occurs in different cellular compartments. Most of these maturational modifications occur at the protein level. The epididymal sperm maturation process was investigated using the ICM-MS (Intact Cell MALDI-TOF MS) approach on boar spermatozoa isolated from fo ...
DOI:
10.1016/j.jprot.2014.09.031

3. A proteomic analysis of p53-independent induction of apoptosis by bortezomib in 4T1 breast cancer cell line

Author:
Yerlikaya, Azmi; Okur, Emrah; Baykal, Ahmet Tarık; Acılan, Ceyda; Boyacı, İhsan; Ulukaya, Engin
Source:
Journal of proteomics 2015 v.113 pp. 315-325
ISSN:
1874-3919
Subject:
DNA replication; antineoplastic agents; apoptosis; breast neoplasms; cytoplasm; energy; gene expression regulation; humans; mechanism of action; models; myeloma; neoplasm cells; nucleic acids; post-translational modification; pro-apoptotic proteins; proteasome endopeptidase complex; protein folding; proteolysis; proteomics; tumor suppressor protein p53; ubiquitin
Abstract:
... The 26S proteasome is a proteolytic enzyme found in both cytoplasm and nucleus. In this study, we examined the differential expression of proteasome inhibitor bortezomib-induced proteins in p53-deficient 4T1 cells. It was found that GRP78 and TCEB2 were over-expressed in response to treatment with bortezomib for 24h. Next, we analyzed the expression of intracellular proteins in response to treatme ...
DOI:
10.1016/j.jprot.2014.09.010

4. A proteomic approach to monitor the dynamic response of the female oviductal epithelial cell surface to male gametes

Author:
Artemenko, Konstantin; Horáková, Jana; Steinberger, Birgit; Besenfelder, Urban; Brem, Gottfried; Bergquist, Jonas; Mayrhofer, Corina
Source:
Journal of proteomics 2015 v.113 pp. 1-14
ISSN:
1874-3919
Subject:
biotin; biotinylation; embryogenesis; epithelial cells; epithelium; females; germ cells; insemination; males; mass spectrometry; oviducts; peptides; protein synthesis; proteome; proteomics; rabbits; semen; signal transduction; surface proteins
Abstract:
... Sophisticated strategies to analyze cell surface proteins are indispensable to study fundamental biological processes, such as the response of cells to environmental changes or cell–cell communication. Herein, we describe a refined mass spectrometry-based approach for the specific characterization and quantitation of cell surface proteins expressed in the female reproductive tract. The strategy is ...
DOI:
10.1016/j.jprot.2014.09.016

5. Comparative proteomic analysis of Arthrobacter phenanthrenivorans Sphe3 on phenanthrene, phthalate and glucose

Author:
Vandera, Elpiniki; Samiotaki, Martina; Parapouli, Maria; Panayotou, George; Koukkou, Anna Irini
Source:
Journal of proteomics 2015 v.113 pp. 73-89
ISSN:
1874-3919
Subject:
Arthrobacter; amino acid metabolism; bacteria; biodegradation; bioremediation; carbon; cell membranes; cell walls; creosote; data analysis; energy; engineering; enzymes; genes; genomics; glucose; glyoxylate cycle; phenanthrene; phthalates; polluted soils; prediction; proteins; proteomics; stress response; transcription (genetics); uptake mechanisms; Greece
Abstract:
... In the present study, by applying comparative quantitative proteomics, we investigated the metabolic adaptation of Arthrobacter phenanthrenivorans Sphe3 when using phenanthrene, phthalate, glucose or glucose plus phenanthrene as sole carbon and energy sources.More than a third of the total Sphe3 proteins, with function prediction within the genome, were identified with confidence. Proteomic analys ...
DOI:
10.1016/j.jprot.2014.08.018

6. Comparative proteomic and physiological analyses reveal the protective effect of exogenous calcium on the germinating soybean response to salt stress

Author:
Yin, Yongqi; Yang, Runqiang; Han, Yongbin; Gu, Zhenxin
Source:
Journal of proteomics 2015 v.113 pp. 110-126
ISSN:
1874-3919
Subject:
Glycine max; antioxidants; biomass; calcium; cluster analysis; cotyledons; endoplasmic reticulum; energy; functional foods; gamma-aminobutyric acid; glutamic acid; methionine; nucleoside-diphosphate kinase; polyamines; protective effect; protein synthesis; proteolysis; proteomics; salt stress; salt tolerance; secondary metabolites; seed germination; signal transduction; soybeans; storage proteins; stress response; stress tolerance
Abstract:
... Calcium enhances salt stress tolerance of soybeans. Nevertheless, the molecular mechanism of calcium's involvement in resistance to salt stress is unclear. A comparative proteomic approach was used to investigate protein profiles in germinating soybeans under NaCl-CaCl2 and NaCl-LaCl3 treatments. A total of 80 proteins affected by calcium in 4-day-old germinating soybean cotyledons and 71 in embry ...
DOI:
10.1016/j.jprot.2014.09.023

7. Determining the Plasmodium vivax VCG-1 strain blood stage proteome

Author:
Moreno-Pérez, D.A.; Dégano, R.; Ibarrola, N.; Muro, A.; Patarroyo, M.A.
Source:
Journal of proteomics 2015 v.113 pp. 268-280
ISSN:
1874-3919
Subject:
Plasmodium vivax; animal models; blood; databases; in vitro culture; malaria; parasites; prediction; proteins; proteome; proteomics; public health; vaccines; virulence
Abstract:
... Plasmodium vivax is the second most prevalent parasite species causing malaria in humans living in tropical and subtropical areas throughout the world. There have been few P. vivax proteomic studies to date and they have focused on using clinical isolates, given the technical difficulties concerning how to maintain an in vitro culture of this species. This study was thus focused on identifying the ...
DOI:
10.1016/j.jprot.2014.10.003

8. Differential proteomic profiling unveils new molecular mechanisms associated with mitochondrial complex III deficiency

Author:
Marín-Buera, Lorena; García-Bartolomé, Alberto; Morán, María; López-Bernardo, Elia; Cadenas, Susana; Hidalgo, Beatriz; Sánchez, Ricardo; Seneca, Sara; Arenas, Joaquín; Martín, Miguel A.; Ugalde, Cristina
Source:
Journal of proteomics 2015 v.113 pp. 38-56
ISSN:
1874-3919
Subject:
adenosine triphosphate; amino acids; autophagy; cluster analysis; cytoskeleton; electron transport chain; enzyme activity; fibroblasts; gene expression regulation; genes; glycolysis; humans; mass spectrometry; mitochondria; mutants; mutation; oxygen consumption; patients; protein metabolism; proteins; proteomics; skin (animal); stress response; ubiquinol-cytochrome-c reductase
Abstract:
... We have analyzed the cellular pathways and metabolic adaptations that take place in primary skin fibroblasts from patients with mutations in BCS1L, a major genetic cause of mitochondrial complex III enzyme deficiency. Mutant fibroblasts exhibited low oxygen consumption rates and intracellular ATP levels, indicating that the main altered molecular event probably is a limited respiration-coupled ATP ...
DOI:
10.1016/j.jprot.2014.09.007
PubMed:
25239759
PubMed Central:
PMC4259860

9. Effect of CDNB on filarial thioredoxin reductase : A proteomic and biochemical approach

Author:
Tiwari, Savitri; Wadhawan, Mohit; Singh, Neetu; Rathaur, Sushma
Source:
Journal of proteomics 2015 v.113 pp. 435-446
ISSN:
1874-3919
Subject:
Brugia malayi; Setaria cervi; Wuchereria bancrofti; adenosine triphosphate; adults; antioxidants; apoptosis; caspase-3; cattle; cytochrome-c oxidase; death; drug therapy; drugs; filariasis; glycolysis; homeostasis; larvae; lipid peroxidation; membrane permeability; mitochondria; mitochondrial membrane; nematicides; oxidative stress; parasites; phosphopyruvate hydratase; proteins; proteomics; screening; tyrosine; viability
Abstract:
... Thioredoxin reductase plays a crucial role in the maintenance of cellular redox homeostasis. In this study, we have targeted TrxR in Setaria cervi, a bovine filarial parasite using its inhibitor CDNB. It caused significant decrease in the motility and viability of these parasites leading to their death. Inhibition of TrxR leads to the downregulation of the antioxidant system followed by generation ...
DOI:
10.1016/j.jprot.2014.10.007

10. Effect of vancomycin on the proteome of the multiresistant Enterococcus faecium SU18 strain

Author:
Ramos, Sónia; Chafsey, Ingrid; Silva, Nuno; Hébraud, Michel; Santos, Hugo; Capelo-Martinez, José-Luis; Poeta, Patrícia; Igrejas, Gilberto
Source:
Journal of proteomics 2015 v.113 pp. 378-387
ISSN:
1874-3919
Subject:
Enterococcus faecium; antibiotic resistance; bacteria; cell walls; dipeptidases; drugs; energy metabolism; gene expression regulation; glyceraldehyde-3-phosphate dehydrogenase; glycopeptides; pathogens; protein synthesis; proteins; proteome; proteomics; triose-phosphate isomerase; vancomycin
Abstract:
... Enterococci are not highly pathogenic bacteria, but the incidence of vancomycin resistance among clinical isolates of this microbial group is steadily increasing, posing a threat to public health. Vancomycin-resistant enterococci are currently some of the most recalcitrant hospital-associated pathogens against which new therapies are urgently needed. To understand the molecular mechanisms of bacte ...
DOI:
10.1016/j.jprot.2014.10.012

11. Identification of anti-metastatic drug and natural compound targets in isogenic colorectal cancer cells

Author:
Lee, Jin-Gyun; McKinney, Kimberly Q.; Pavlopoulos, Antonis J.; Park, Jeong-Hill; Hwang, Sunil
Source:
Journal of proteomics 2015 v.113 pp. 326-336
ISSN:
1874-3919
Subject:
Western blotting; cell movement; cell proliferation; colorectal neoplasms; cumulative risk; curcumin; data collection; drug therapy; drugs; fatty-acid synthase; genes; histones; immunohistochemistry; metastasis; neoplasm cells; proteomics; small interfering RNA; transfection
Abstract:
... Therapeutic strategies for cancer treatment often remain challenging due to the cumulative risk derived from metastasis, which has been described as an aggressive state of cancer cell proliferation often resulting in failure of clinical therapy. In the current study, anti-metastatic properties of three chemotherapeutic drugs and three compounds from natural sources were investigated by comparative ...
DOI:
10.1016/j.jprot.2014.10.009

12. Identification of membrane proteins associated with phenylpropanoid tolerance and transport in Escherichia coli BL21

Author:
Zhou, Jingwen; Wang, Kui; Xu, Sha; Wu, Junjun; Liu, Peiran; Du, Guocheng; Li, Jianghua; Chen, Jian
Source:
Journal of proteomics 2015 v.113 pp. 15-28
ISSN:
1874-3919
Subject:
Escherichia coli; cell membranes; cytotoxicity; gene expression regulation; gene overexpression; genetic engineering; genetically engineered microorganisms; hosts; metabolic engineering; microorganisms; naringenin; outer membrane proteins; phenylpropanoids; physiological transport; phytopharmaceuticals; proteomics; resveratrol; rutin; transporters
Abstract:
... Phenylpropanoids are phytochemicals produced by some plants and possess a wide variety of biological activities. These compounds exist in plants in low amounts. Production of them in genetically engineered microorganisms has many advantages. A majority of functional phenylpropanoids are toxic to microbial hosts. Export of these compounds may relieve the cellular toxicity and increase the yield. Ho ...
DOI:
10.1016/j.jprot.2014.09.012

13. Identification of novel post-translational modifications in linker histones from chicken erythrocytes

Author:
Sarg, Bettina; Lopez, Rita; Lindner, Herbert; Ponte, Inma; Suau, Pedro; Roque, Alicia
Source:
Journal of proteomics 2015 v.113 pp. 162-177
ISSN:
1874-3919
Subject:
acetylation; centrifugation; chickens; chromatin; erythrocytes; gene expression regulation; histones; humans; mass spectrometry; mice; micrococcal nuclease; oxidation; peptides; phosphorylation; post-translational modification; rats; sequence alignment
Abstract:
... Chicken erythrocyte nuclei were digested with micrococcal nuclease and fractionated by centrifugation in low-salt buffer into soluble and insoluble fractions. Post-translational modifications of the purified linker histones of both fractions were analyzed by LC–ESI–MS/MS. All six histone H1 subtypes (H1.01, H1.02, H1.03, H1.10, H1.1L and H1.1R) and histone H5 were identified. Mass spectrometry ana ...
DOI:
10.1016/j.jprot.2014.10.004

14. iTRAQ-based quantitative proteomics analysis of Brassica napus leaves reveals pathways associated with chlorophyll deficiency

Author:
Chu, Pu; Yan, Gui Xia; Yang, Qing; Zhai, Li Na; Zhang, Cheng; Zhang, Feng Qi; Guan, Rong Zhan
Source:
Journal of proteomics 2015 v.113 pp. 244-259
ISSN:
1874-3919
Subject:
Brassica napus; DNA; biosynthesis; carbon dioxide fixation; chlorophyll; chromatin; crop yield; crops; energy transfer; enzymes; epigenetics; ethyl methanesulfonate; gene expression regulation; histones; homeostasis; leaves; lipid peroxidation; messenger RNA; methylation; monitoring; mutants; photosynthesis; plant growth; proteome; proteomics; secondary metabolites; spliceosomes
Abstract:
... Photosynthesis, the primary source of plant biomass, is important for plant growth and crop yield. Chlorophyll is highly abundant in plant leaves and plays essential roles in photosynthesis. We recently isolated a chlorophyll-deficient mutant (cde1) from ethyl methanesulfonate (EMS) mutagenized Brassica napus. Herein, quantitative proteomics analysis using the iTRAQ approach was conducted to inves ...
DOI:
10.1016/j.jprot.2014.10.005

15. Metabolic reprogramming in transformed mouse cortical astrocytes: A proteomic study

Author:
Bentaib, Azeddine; De Tullio, Pascal; Chneiweiss, Hervé; Hermans, Emmanuel; Junier, Marie-Pierre; Leprince, Pierre
Source:
Journal of proteomics 2015 v.113 pp. 292-314
ISSN:
1874-3919
Subject:
astrocytes; biosynthesis; carbohydrates; electrophoresis; enzyme activity; glutamic acid; glutaminase; glycolysis; isozymes; lactic acid; lipids; metabolites; mice; models; neoplasm cells; neoplasms; neurotransmitters; nucleotides; nutrients; oxidation; oxidative stress; phenotype; protein synthesis; proteomics; pyruvate carboxylase; structural proteins
Abstract:
... Metabolic reprogramming is thought to play a key role in sustaining the survival and proliferation of cancer cells. These changes facilitate for example the uptake and release of nutrients required for nucleotide, protein and lipid synthesis necessary for macromolecule assembly and tumor growth. We applied a 2D-DIGE (two-dimensional differential in-gel electrophoresis) quantitative proteomic analy ...
DOI:
10.1016/j.jprot.2014.09.019

16. Metabolite and proteome changes during the ripening of Syrah and Cabernet Sauvignon grape varieties cultured in a nontraditional wine region in Brazil

Author:
Fraige, Karina; González-Fernández, Raquel; Carrilho, Emanuel; Jorrín-Novo, Jesús V.
Source:
Journal of proteomics 2015 v.113 pp. 206-225
ISSN:
1874-3919
Subject:
Vitis vinifera; adulterated products; anthocyanins; cultivation area; enzymes; genes; grapes; malic acid; metabolism; metabolites; multivariate analysis; proteins; proteome; proteomics; provenance; ripening; sugars; two-dimensional gel electrophoresis; wine cultivars; wines; Brazil
Abstract:
... Grapevines are an important fruit crop from economic and cultural point of views in many countries, including Brazil, where the practice of vitiviniculture is being developed in different regions. We compared the anthocyanin concentration, the main organic acids and sugars, and the proteome profiles during berry ripening of Syrah and Cabernet Sauvignon grapes from two distinct geographical sources ...
DOI:
10.1016/j.jprot.2014.09.021

17. NanoUPLC/MSE proteomic analysis reveals modulation on left ventricle proteome from hypertensive rats after exercise training

Author:
Petriz, Bernardo A.; Almeida, Jeeser A.; Gomes, Clarissa P.C.; Pereira, Rinaldo W.; Murad, André M.; Franco, Octavio L.
Source:
Journal of proteomics 2015 v.113 pp. 351-365
ISSN:
1874-3919
Subject:
adenine; animal disease models; antioxidants; desmin; exercise; glutathione; heart failure; hemoglobin; histones; hypertension; hypertrophy; mortality; myosin light chains; phosphopyruvate hydratase; proteome; proteomics; rats; risk factors; serum albumin; systolic blood pressure; transcription factors; tropomyosins
Abstract:
... NanoUPLC/MSᴱ was used to verify the effects of 8weeks of low (SHR-LIT=4) and high (SHR-HIT=4) intensity training over the left ventricle proteome of hypertensive rats (SHR-C=4). Training enhanced the aerobic capacity and reduced the systolic blood pressure in all exercised rats. NanoUPLC/MSᴱ identified 250 proteins, with 233 in common to all groups and 16 exclusive to SHR-C, 2 to SHR-LIT, and 2 to ...
DOI:
10.1016/j.jprot.2014.10.010

18. Parallel reaction monitoring (PRM) and selected reaction monitoring (SRM) exhibit comparable linearity, dynamic range and precision for targeted quantitative HDL proteomics

Author:
Ronsein, Graziella E.; Pamir, Nathalie; von Haller, Priska D.; Kim, Daniel S.; Oda, Michael N.; Jarvik, Gail P.; Vaisar, Tomas; Heinecke, Jay W.
Source:
Journal of proteomics 2015 v.113 pp. 388-399
ISSN:
1874-3919
Subject:
apolipoprotein A-I; cardioprotective effect; cardiovascular diseases; cholesterol; high density lipoprotein; ions; monitoring; nanoparticles; proteome; proteomics; risk; risk assessment; translation (genetics)
Abstract:
... High-density lipoprotein (HDL), a lipid nanoparticle containing many different low abundance proteins, is an attractive target for clinical proteomics because its compositional heterogeneity is linked to its cardioprotective effects. Selected reaction monitoring (SRM) is currently the method of choice for targeted quantification of proteins in such a complex biological matrix. However, model syste ...
DOI:
10.1016/j.jprot.2014.10.017
PubMed:
25449833
PubMed Central:
PMC4259393

19. Proteomic analysis of oil bodies in mature Jatropha curcas seeds with different lipid content

Author:
Liu, Hui; Wang, Cuiping; Chen, Fan; Shen, Shihua
Source:
Journal of proteomics 2015 v.113 pp. 403-414
ISSN:
1874-3919
Subject:
Jatropha curcas; RNA-binding proteins; calcium; economic valuation; gels; glycine (amino acid); leptin; lipid bodies; lipid content; lipids; mutants; oils; oilseed crops; oleosin; proteome; proteomics; seeds; statistical analysis; structural proteins; two-dimensional gel electrophoresis
Abstract:
... To reveal the difference among three mature Jatropha curcas seeds (JcVH, variant with high lipid content; JcW, wild type and JcVL, variant with low lipid content) with different lipid content, comparative proteomics was employed to profile the changes of oil body (OB) associated protein species by using gels-based proteomic technique. Eighty-three protein species were successfully identified throu ...
DOI:
10.1016/j.jprot.2014.10.013

20. Proteomic analysis of responsive stem proteins of resistant and susceptible cashew plants after Lasiodiplodia theobromae infection

Author:
Cipriano, Aline K.A.L.; Gondim, Darcy M.F.; Vasconcelos, Ilka M.; Martins, Jorge A.M.; Moura, Arlindo A.; Moreno, Frederico B.; Monteiro-Moreira, Ana C.O.; Melo, Jose G.M.; Cardoso, Jose E.; Paiva, Ana Luiza S.; Oliveira, Jose T.A.
Source:
Journal of proteomics 2015 v.113 pp. 90-109
ISSN:
1874-3919
Subject:
Anacardium occidentale; Lasiodiplodia theobromae; disease incidence; disease resistance; energy metabolism; fungal diseases of plants; fungi; genotype; gummosis; homeostasis; open pollination; orchards; pathogens; protein metabolism; proteins; proteome; proteomics; stems; two-dimensional gel electrophoresis; Brazil
Abstract:
... Gummosis is an aggressive disease caused by the necrotrophic fungus Lasiodiplodia theobromae (Pat.) Griffon & Maubl that threatens commercial cashew orchads in Brazil. To study the molecular mechanisms involved in the cashew response to L. theobromae, a proteomic analysis of stems from the commercial cashew clone BRS 226 (resistant) was conducted at early times post-artificial infection. In additi ...
DOI:
10.1016/j.jprot.2014.09.022