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- Hashii, Noritaka, et al. Show all 4 Authors
- Journal of proteomics 2014 v.101 pp. 17-30
- acetone; affinity chromatography; blood serum; data collection; glycopeptides; glycoproteins; glycosylation; humans; hydrophobicity; lectins; solubility
- ... LC/MS is commonly used for site-specific glycosylation analysis of glycoproteins in cells and tissues. A limitation of this technique is the difficulty in acquiring reliable mass spectra for glycopeptides, mainly due to their high heterogeneity and poor hydrophobicity. Here, we establish a versatile method for efficient glycopeptide enrichment to acquire reliable mass spectra. Several lines of evi ...