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1. Improvements on the quantitative analysis of Trypanosoma cruzi histone post translational modifications: Study of changes in epigenetic marks through the parasite's metacyclogenesis and life cycle

Author:
de Lima, Loyze P; Poubel, Saloe Bispo; Yuan, Zuo-Fei; Rosón, Juliana Nunes; Vitorino, Francisca Nathalia de Luna; Holetz, Fabiola Barbieri; Garcia, Benjamin A.; da Cunha, Julia Pinheiro Chagas
Source:
Journal of proteomics 2020 v.225 pp. 103847
ISSN:
1874-3919
Subject:
Chagas disease; Trypanosoma cruzi; acetylation; chromatin; chromosome morphology; computer software; data collection; disease control; epigenetics; epimastigotes; eukaryotic cells; genes; histones; mass spectrometry; messenger RNA; methylation; parasites; peptides; post-translational modification; protein content; quantitative analysis; transcription (genetics); trypomastigotes
Abstract:
... Trypanosome histone N-terminal sequences are very divergent from the other eukaryotes, although they are still decorated by post-translational modifications (PTMs). Here, we used a highly robust workflow to analyze histone PTMs in the parasite Trypanosoma cruzi using mass spectrometry-based (MS-based) data-independent acquisition (DIA). We adapted the workflow for the analysis of the parasite's hi ...
DOI:
10.1016/j.jprot.2020.103847

2. A high glucose level is associated with decreased aspirin-mediated acetylation of platelet cyclooxygenase (COX)-1 at serine 529: A pilot study

Author:
Finamore, Francesco; Reny, Jean-Luc; Malacarne, Sarah; Fontana, Pierre; Sanchez, Jean-Charles
Source:
Journal of proteomics 2019 v.192 pp. 258-266
ISSN:
1874-3919
Subject:
acetylation; aspirin; blood platelets; cardiovascular diseases; diabetes; glucose; glycation; hyperglycemia; mass spectrometry; patients; prostaglandin synthase; proteins; risk factors; serine
Abstract:
... Diabetes is a major risk factor for cardiovascular diseases. Although aspirin is considered a cornerstone of the prevention and treatment of atherothrombotic-related ischemic events, this antiplatelet drug appears to be less effective in patients with poorly controlled diabetes. It has been suggested that the glycation of platelet proteins plays a pivotal role in poor responsiveness to aspirin. Ho ...
DOI:
10.1016/j.jprot.2018.09.007

3. Mass spectrometry and DigiWest technology emphasize protein acetylation profile from Quisinostat-treated HuT78 CTCL cell line

Author:
Méhul, Bruno; Perrin, Agnes; Grisendi, Karine; Galindo, Antonio Núñez; Dayon, Loïc; Ménigot, Corinne; Rival, Yves; Voegel, Johannes J.
Source:
Journal of proteomics 2018 v.187 pp. 126-143
ISSN:
1874-3919
Subject:
T-cell lymphoma; acetylation; apoptosis; blood; cell lines; drugs; epigenetics; glycine hydroxymethyltransferase; histone deacetylase; histones; immunoassays; mass spectrometry; phosphoglycerate kinase; protein composition; proteome; proteomics; proto-oncogenes; research programs; therapeutics
Abstract:
... Histone deacetylases (HDACs) are key enzymes involved in epigenetic modulation and were targeted by HDAC inhibitors (HDACis) for cancer treatment. The action of HDACis is not restricted to histones and also prevents deacetylation of other proteins, supporting their wide biological actions. The HuT78 cell line is recognized as a key tool to support and understand cutaneous T-cell lymphoma (CTCL) bi ...
DOI:
10.1016/j.jprot.2018.07.003

4. Comprehensive analysis of the lysine acetylome and its potential regulatory roles in the virulence of Streptococcus pneumoniae

Author:
Liu, Yu-Tong; Pan, Ying; Lai, Fubin; Yin, Xing-feng; Ge, Ruiguang; He, Qing-Yu; Sun, Xuesong
Source:
Journal of proteomics 2018 v.176 pp. 46-55
ISSN:
1874-3919
Subject:
Streptococcus pneumoniae; acetylation; bacteria; bioinformatics; gene expression; human diseases; lysine; mass spectrometry; metabolism; proteins; proteome; proteomics; therapeutics; virulence
Abstract:
... Protein lysine acetylation is a well-known modification with vital regulatory roles in various biological processes. Currently, the acetylated proteome in Streptococcus pneumoniae (S. pneumoniae) is not yet clear. Combining immune-affinity enrichment with mass spectrometry-based proteomics, we identified the first lysine acetylome of S. pneumoniae. In total, 653 lysine acetylated sites on 392 prot ...
DOI:
10.1016/j.jprot.2018.01.014

5. A glimpse into the modulation of post-translational modifications of human-colonizing bacteria

Author:
Bastos, Paulo André Dias; da Costa, João Pinto; Vitorino, Rui
Source:
Journal of proteomics 2017 v.152 pp. 254-275
ISSN:
1874-3919
Subject:
acetylation; bacteria; human health; mass spectrometry; peptides; phylogeny; post-translational modification; proteins; proteome; proteomics
Abstract:
... Protein post-translational modifications (PTMs) are a key bacterial feature that holds the capability to modulate protein function and responses to environmental cues. Until recently, their role in the regulation of prokaryotic systems has been largely neglected. However, the latest developments in mass spectrometry-based proteomics have allowed an unparalleled identification and quantification of ...
DOI:
10.1016/j.jprot.2016.11.005

6. Lysine acetylation and cancer: A proteomics perspective

Author:
Gil, Jeovanis; Ramírez-Torres, Alberto; Encarnación-Guevara, Sergio
Source:
Journal of proteomics 2017 v.150 pp. 297-309
ISSN:
1874-3919
Subject:
acetylation; acetyltransferases; antibodies; carcinogenesis; histones; humans; lysine; mass spectrometry; neoplasms; peptides; precipitin tests; proteomics; stoichiometry
Abstract:
... Lysine acetylation is a reversible modification controlled by two groups of enzymes: lysine acetyltransferases (KATs) and lysine deacetylases (KDACs). Acetylated lysine residues are recognized by bromodomains, a family of evolutionarily conserved domains. The use of high-resolution mass spectrometry-based proteomics, in combination with the enrichment of acetylated peptides through immunoprecipita ...
DOI:
10.1016/j.jprot.2016.10.003

7. Proteome-wide analysis of lysine acetylation in adult Schistosoma japonicum worm

Author:
Hong, Yang; Cao, Xiaodan; Han, Qian; Yuan, Chunxiu; Zhang, Min; Han, Yanhui; Zhu, Chuangang; Lin, Tao; Lu, Ke; Li, Hao; Fu, Zhiqiang; Lin, Jiaojiao
Source:
Journal of proteomics 2016 v.148 pp. 202-212
ISSN:
1874-3919
Subject:
Schistosoma japonicum; acetylation; adults; bioinformatics; catalytic activity; cytoskeleton; enzymes; eukaryotic cells; gene expression; lysine; mass spectrometry; parasites; post-translational modification; prokaryotic cells; proteins; schistosomiasis; surveys; vaccines; zoonoses
Abstract:
... Lysine acetylation, a ubiquitous and conserved posttranslational modification, has recently been shown to participate in many diverse non-chromatin-associated biological processes in prokaryotes and eukaryotes. However, the full extent and functional significance of acetylation in Schistosoma japonicum is still unknown. To investigate the nature, extent, and biological functions of lysine acetylat ...
DOI:
10.1016/j.jprot.2016.08.008

8. Global-scale profiling of differential expressed lysine acetylated proteins in colorectal cancer tumors and paired liver metastases

Author:
Shen, Zhanlong; Wang, Bo; Luo, Jianyuan; Jiang, Kewei; Zhang, Hui; Mustonen, Harri; Puolakkainen, Pauli; Zhu, Jun; Ye, Yingjiang; Wang, Shan
Source:
Journal of proteomics 2016 v.142 pp. 24-32
ISSN:
1874-3919
Subject:
acetylation; biochemical pathways; colorectal neoplasms; gene expression regulation; histones; liver; lysine; mass spectrometry; metastasis; peptides; protein synthesis; therapeutics
Abstract:
... Lysine acetylated modification was indicated to impact colorectal cancer (CRC)’s distant metastasis. However, the global acetylated proteins in CRC and the differential expressed acetylated proteins and acetylated sites between CRC primary and distant metastatic tumor remains unclear. Our aim was to construct a complete atlas of acetylome in CRC and paired liver metastases. Combining high affinity ...
DOI:
10.1016/j.jprot.2016.05.002

9. Revealing the functional structure of a new PLA2 K49 from Bothriopsis taeniata snake venom employing automatic “de novo” sequencing using CID/HCD/ETD MS/MS analyses

Author:
Carregari, Victor Corasolla; Dai, Jie; Verano-Braga, Thiago; Rocha, Thalita; Ponce-Soto, Luis Alberto; Marangoni, Sergio; Roepstorff, Peter
Source:
Journal of proteomics 2016 v.131 pp. 131-139
ISSN:
1874-3919
Subject:
acetylation; chromatography; creatine kinase; hydrophobicity; lysine; mass spectrometry; medicinal properties; muscles; phospholipases; phospholipids; proteins; snakes; venoms
Abstract:
... Snake venoms are composed of approximately 90% of proteins with several pharmacological activities having high potential in research as biological tools. One of the most abundant compounds is phospholipases A2 (PLA2), which are the most studied venom protein due to their wide pharmacological activity. Using a combination of chromatographic steps, a new PLA2 K49 was isolated and purified from the w ...
DOI:
10.1016/j.jprot.2015.10.020

10. Lysine acetylation is a common post-translational modification of key metabolic pathway enzymes of the anaerobe Porphyromonas gingivalis

Author:
Butler, Catherine A.; Veith, Paul D.; Nieto, Matthew F.; Dashper, Stuart G.; Reynolds, Eric C.
Source:
Journal of proteomics 2015 v.128 pp. 352-364
ISSN:
1874-3919
Subject:
Porphyromonas gingivalis; acetylation; bacteria; biochemical pathways; chronic diseases; energy; enzymes; eukaryotic cells; fermentation; lysine; mass spectrometry; pathogens; peptides; periodontitis; post-translational modification; prokaryotic cells; proteins; proteomics; virulence
Abstract:
... Porphyromonas gingivalis is a Gram-negative anaerobe considered to be a keystone pathogen in the development of the bacterial-associated inflammatory oral disease chronic periodontitis. Although post-translational modifications (PTMs) of proteins are commonly found to modify protein function in eukaryotes and prokaryotes, PTMs such as lysine acetylation have not been examined in P. gingivalis. Lys ...
DOI:
10.1016/j.jprot.2015.08.015

11. Characterisation of the influences of aspirin-acetylation and glycation on human plasma proteins

Author:
Finamore, Francesco; Priego-Capote, Feliciano; Nolli, Severine; Zufferey, Anne; Fontana, Pierre; Sanchez, Jean-Charles
Source:
Journal of proteomics 2015 v.114 pp. 125-135
ISSN:
1874-3919
Subject:
Western blotting; acetylation; aspirin; blood; blood proteins; developmental stages; diabetes; glucose; glycation; humans; mass spectrometry; models; post-translational modification; proteomics; quantitative analysis
Abstract:
... The competition effect between aspirin-mediated acetylation and protein glycation has been a matter of concern for decades. However, the exact interactions between these two post-translational modifications are still not well understood. Several efforts have been made to explain how aspirin prevents glycation, but the influence of prior protein glycation on the action of aspirin has never been inv ...
DOI:
10.1016/j.jprot.2014.11.005

12. Identification of novel post-translational modifications in linker histones from chicken erythrocytes

Author:
Sarg, Bettina; Lopez, Rita; Lindner, Herbert; Ponte, Inma; Suau, Pedro; Roque, Alicia
Source:
Journal of proteomics 2015 v.113 pp. 162-177
ISSN:
1874-3919
Subject:
acetylation; centrifugation; chickens; chromatin; erythrocytes; gene expression regulation; histones; humans; mass spectrometry; mice; micrococcal nuclease; oxidation; peptides; phosphorylation; post-translational modification; rats; sequence alignment
Abstract:
... Chicken erythrocyte nuclei were digested with micrococcal nuclease and fractionated by centrifugation in low-salt buffer into soluble and insoluble fractions. Post-translational modifications of the purified linker histones of both fractions were analyzed by LC–ESI–MS/MS. All six histone H1 subtypes (H1.01, H1.02, H1.03, H1.10, H1.1L and H1.1R) and histone H5 were identified. Mass spectrometry ana ...
DOI:
10.1016/j.jprot.2014.10.004

13. Comparative analysis of histone H3 and H4 post-translational modifications of esophageal squamous cell carcinoma with different invasive capabilities

Author:
Zhang, Kai; Li, Liyan; Zhu, Mengxiao; Wang, Guojuan; Xie, Jianjun; Zhao, Yunlong; Fan, Enguo; Xu, Liyan; Li, Enmin
Source:
Journal of proteomics 2015 v.112 pp. 180-189
ISSN:
1874-3919
Subject:
DNA; acetylation; carcinogenesis; cell culture; chromatin; data collection; esophageal neoplasms; esophagus; histones; humans; immunohistochemistry; isotope labeling; lysine; mass spectrometry; methylation; patients; phenotype; post-translational modification; proteomics; squamous cell carcinoma; stable isotopes; transcription (genetics)
Abstract:
... Eukaryotic DNA is packaged into a chromatin with the help of four core histones (H2A, H2B, H3, and H4). Diverse histone post-translational modifications (PTMs) are hence involved in the regulation of gene transcription. However, how this regulation does work is still poorly understood and lacks details. Here we used the mass spectrometry-based proteomics approach to perform a comparative analysis ...
DOI:
10.1016/j.jprot.2014.09.004

14. Phagocytosis of nano-sized titanium dioxide triggers changes in protein acetylation

Author:
Sund, Jukka; Palomäki, Jaana; Ahonen, Niina; Savolainen, Kai; Alenius, Harri; Puustinen, Anne
Source:
Journal of proteomics 2014 v.108 pp. 469-483
ISSN:
1874-3919
Subject:
acetylation; cathepsin D; coatings; cytoskeleton; gel electrophoresis; homeostasis; humans; inflammation; lysine; macrophages; mass spectrometry; mice; monocytes; nanoparticles; oxidative stress; phagocytosis; phosphorylation; post-translational modification; proteins; proteome; proteomics; risk; silica; titanium dioxide
Abstract:
... Nano-sized titanium dioxide (nTiO2) is one of the most produced engineered nanomaterials and therefore carries a high risk for workplace exposure. In several nanosafety studies, exposure to nTiO2 has been shown to trigger inflammation in mice lung and to cause oxidative stress. Here, cytoplasmic proteome changes in human monocyte derived macrophages were investigated with two-dimensional differenc ...
DOI:
10.1016/j.jprot.2014.06.011

15. Unexpected extensive lysine acetylation in the trump-card antibiotic producer Streptomyces roseosporus revealed by proteome-wide profiling

Author:
Liao, Guojian; Xie, Longxiang; Li, Xin; Cheng, Zhongyi; Xie, Jianping
Source:
Journal of proteomics 2014 v.106 pp. 260-269
ISSN:
1874-3919
Subject:
Streptomyces roseosporus; acetylation; bacteria; bioactive properties; bioinformatics; biosynthesis; daptomycin; enzymes; gene expression; genes; lysine; mass spectrometry; post-translational modification; prokaryotic cells; proteins; proteomics; secondary metabolites; siderophores; trophic relationships
Abstract:
... Lysine acetylation is emerging as a ubiquitous and conserved posttranslational modification in living cells. While the role of lysine acetylation in regulating primary metabolism is well-established, its function in secondary metabolism remains largely elusive. To gain insight into the nature, extent and biological function of lysine acetylation in Streptomyces reseosporus, a producer of daptomyci ...
DOI:
10.1016/j.jprot.2014.04.017

16. Beyond gene expression: The impact of protein post-translational modifications in bacteria

Author:
Cain, Joel A.; Solis, Nestor; Cordwell, Stuart J.
Source:
Journal of proteomics 2014 v.97 pp. 265-286
ISSN:
1874-3919
Subject:
acetylation; bacteria; bacterial proteins; carbohydrates; eukaryotic cells; gene expression; lipids; mass spectrometry; methylation; pathophysiology; peptides; phosphorylation; post-translational modification; proteolysis; proteomics
Abstract:
... The post-translational modification (PTM) of proteins plays a critical role in the regulation of a broad range of cellular processes in eukaryotes. Yet their role in governing similar systems in the conventionally presumed ‘simpler’ forms of life has been largely neglected and, until recently, was thought to occur only rarely, with some modifications assumed to be limited to higher organisms alone ...
DOI:
10.1016/j.jprot.2013.08.012

17. Advances in purification and separation of posttranslationally modified proteins

Author:
Černý, Martin; Skalák, Jan; Cerna, Hana; Brzobohatý, Břetislav
Source:
Journal of proteomics 2013 v.92 pp. 2-27
ISSN:
1874-3919
Subject:
acetylation; deamidation; fractionation; genes; glycation; glycosylation; mass spectrometry; methylation; phosphorylation; proteins; proteome; proteomics; ubiquitination
Abstract:
... Posttranslational modifications (PTMs) of proteins represent fascinating extensions of the dynamic complexity of living cells' proteomes. The results of enzymatically catalyzed or spontaneous chemical reactions, PTMs form a fourth tier in the gene – transcript – protein cascade, and contribute not only to proteins' biological functions, but also to challenges in their analysis. There have been tre ...
DOI:
10.1016/j.jprot.2013.05.040
PubMed:
23777897

18. Combined bottom-up and top-down mass spectrometry analyses of the pattern of post-translational modifications of Drosophila melanogaster linker histone H1

Author:
Bonet-Costa, Carles; Vilaseca, Marta; Diema, Claudio; Vujatovic, Olivera; Vaquero, Alejandro; Omeñaca, Núria; Castejón, Lucía; Bernués, Jordi; Giralt, Ernest; Azorín, Fernando
Source:
Journal of proteomics 2012 v.75 no.13 pp. 4124-4138
ISSN:
1874-3919
Subject:
DNA; Drosophila melanogaster; acetylation; heterochromatin; histones; humans; mass spectrometry; methylation; mice; mitosis; nucleosomes; phosphorylation; ubiquitination
Abstract:
... Linker histone H1 is a major chromatin component that binds internucleosomal DNA and mediates the folding of nucleosomes into a higher-order structure, namely the 30-nm chromatin fiber. Multiple post-translational modifications (PTMs) of core histones H2A, H2B, H3 and H4 have been identified and their important contribution to the regulation of chromatin structure and function is firmly establishe ...
DOI:
10.1016/j.jprot.2012.05.034
PubMed:
22647927