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You searched for: Subject "binding capacity" Remove constraint Subject: "binding capacity" Journal Journal of the American Chemical Society Remove constraint Journal: Journal of the American Chemical Society Publication Year 2018 Remove constraint Publication Year: 2018 Subject models Remove constraint Subject: models
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1. Ribosomal Incorporation of Consecutive β-Amino Acids

Author:
Katoh, Takayuki; Suga, Hiroaki
Source:
Journal of the American Chemical Society 2018 v.140 no.38 pp. 12159-12167
ISSN:
1520-5126
Subject:
binding capacity,   etc   ; Escherichia coli; amino acids; drugs; models; nanomaterials; peptides;     Show all 7 Subjects
Abstract:
... Due to their unique characteristics, which are not shared by canonical α-peptides, peptides that contain stretches of consecutive β-amino acids are attractive scaffolds for novel peptide drugs and nanomaterials. Although ribosomal incorporation of single or nonconsecutive β-amino acids into peptides has previously been reported, the incorporation of consecutive β-amino acids has not yet been accom ...
DOI:
10.1021/jacs.8b07247

2. Multivalent Ligand Binding to Cell Membrane Antigens: Defining the Interplay of Affinity, Valency, and Expression Density

Author:
Csizmar, Clifford M.; Petersburg, Jacob R.; Perry, Thomas J.; Rozumalski, Lakmal; Hackel, Benjamin J.; Wagner, Carston R.
Source:
Journal of the American Chemical Society 2018 v.141 no.1 pp. 251-261
ISSN:
1520-5126
Subject:
binding capacity,   etc   ; T-lymphocytes; antigens; cell membranes; engineering; epithelial cell adhesion molecule; fibronectins; ligands; models; neoplasms; therapeutics; tissues;     Show all 12 Subjects
Abstract:
... Nature uses multivalency to govern many biological processes. The development of macromolecular and cellular therapies has largely been dependent on engineering similar polyvalent interactions to enable effective targeting. Such therapeutics typically utilize high-affinity binding domains that have the propensity to recognize both antigen-overexpressing tumors and normal-expressing tissues, leadin ...
DOI:
10.1021/jacs.8b09198

3. Native Electrospray Ionization Mass Spectrometry Reveals Multiple Facets of Aptamer–Ligand Interactions: From Mechanism to Binding Constants

Author:
Gülbakan, Basri; Barylyuk, Konstantin; Schneider, Petra; Pillong, Max; Schneider, Gisbert; Zenobi, Renato
Source:
Journal of the American Chemical Society 2018 v.140 no.24 pp. 7486-7497
ISSN:
1520-5126
Subject:
binding capacity,   etc   ; binding sites; calorimetry; circular dichroism spectroscopy; electrospray ionization mass spectrometry; ligands; models; oligonucleotides; receptors; stoichiometry; titration;     Show all 11 Subjects
Abstract:
... Aptamers are oligonucleotide receptors obtained through an iterative selection process from random-sequence libraries. Though many aptamers for a broad range of targets with high affinity and selectivity have been generated, a lack of high-resolution structural data and the limitations of currently available biophysical tools greatly impede understanding of the mechanisms of aptamer–ligand interac ...
DOI:
10.1021/jacs.7b13044

4. Free-Energy-Based Protein Design: Re-Engineering Cellular Retinoic Acid Binding Protein II Assisted by the Moveable-Type Approach

Author:
Zhong, Haizhen A.; Santos, Elizabeth M.; Vasileiou, Chrysoula; Zheng, Zheng; Geiger, James H.; Borhan, Babak; Merz, Kenneth M.
Source:
Journal of the American Chemical Society 2018 v.140 no.10 pp. 3483-3486
ISSN:
1520-5126
Subject:
binding capacity,   etc   ; Gibbs free energy; amino acids; binding proteins; binding sites; models; molecular dynamics; mutagenesis; mutants; protein engineering; retinoic acid;     Show all 11 Subjects
Abstract:
... How to fine-tune the binding free energy of a small-molecule to a receptor site by altering the amino acid residue composition is a key question in protein engineering. Indeed, the ultimate solution to this problem, to chemical accuracy (±1 kcal/mol), will result in profound and wide-ranging applications in protein design. Numerous tools have been developed to address this question using knowledge ...
DOI:
10.1021/jacs.7b10368

5. Insights into the Structural Complexity of Colloidal CdSe Nanocrystal Surfaces: Correlating the Efficiency of Nonradiative Excited-State Processes to Specific Defects

Author:
Saniepay, Mersedeh; Mi, Chenjia; Liu, Zhihui; Abel, E. Paige; Beaulac, Rémi
Source:
Journal of the American Chemical Society 2018 v.140 no.5 pp. 1725-1736
ISSN:
1520-5126
Subject:
binding capacity,   etc   ; Gibbs free energy; Lewis acids; cadmium; energy conversion; ligands; models; nanocrystals; nuclear magnetic resonance spectroscopy; photoluminescence; semiconductors;     Show all 11 Subjects
Abstract:
... II–VI colloidal semiconductor nanocrystals (NCs), such as CdSe NCs, are often plagued by efficient nonradiative recombination processes that severely limit their use in energy-conversion schemes. While these processes are now well-known to occur at the surface, a full understanding of the exact nature of surface defects and of their role in deactivating the excited states of NCs has yet to be esta ...
DOI:
10.1021/jacs.7b10649

6. Imaging Insulin Secretion from Mouse Pancreas by MRI Is Improved by Use of a Zinc-Responsive MRI Sensor with Lower Affinity for Zn²⁺ Ions

Author:
Martins, André F.; Clavijo Jordan, Veronica; Bochner, Filip; Chirayil, Sara; Paranawithana, Namini; Zhang, Shanrong; Lo, Su-Tang; Wen, Xiaodong; Zhao, Piyu; Neeman, Michal; Sherry, A. Dean
Source:
Journal of the American Chemical Society 2018 v.140 no.50 pp. 17456-17464
ISSN:
1520-5126
Subject:
binding capacity,   etc   ; glucose; granules; histology; human serum albumin; image analysis; insulin; insulin secretion; ions; islets of Langerhans; magnetic resonance imaging; mice; models; moieties; staining; zinc;     Show all 16 Subjects
Abstract:
... It has been demonstrated that divalent zinc ions packaged with insulin in β-cell granules can be detected by MRI during glucose-stimulated insulin secretion using a gadolinium-based Zn²⁺-sensitive agent. This study was designed to evaluate whether a simpler agent design having single Zn²⁺-sensing moieties but with variable Zn²⁺ binding affinities might also detect insulin secretion from the pancre ...
DOI:
10.1021/jacs.8b07607

7. Engineering Improved Antiphosphotyrosine Antibodies Based on an Immunoconvergent Binding Motif

Author:
Mou, Yun; Zhou, Xin X.; Leung, Kevin; Martinko, Alexander J.; Yu, Jiun-Yann; Chen, Wentao; Wells, James A.
Source:
Journal of the American Chemical Society 2018 v.140 no.48 pp. 16615-16624
ISSN:
1520-5126
Subject:
binding capacity,   etc   ; Western blotting; antibodies; crystal structure; engineering; eukaryotic cells; fluorescent antibody technique; genes; germ cells; hydrogen bonding; mice; models; phosphates; post-translational modification; sequence alignment; signal transduction;     Show all 16 Subjects
Abstract:
... Phosphotyrosine (pY) is one of the most highly studied posttranslational modifications that is responsible for tightly regulating many signaling pathways in eukaryotes. Pan-specific pY antibodies have emerged as powerful tools for understanding the role of these modifications. Nevertheless, structures have not been reported for pan-specific pY antibodies, greatly impeding the further development o ...
DOI:
10.1021/jacs.8b08402

8. Probing Antibody Binding to Canine Parvovirus with Charge Detection Mass Spectrometry

Author:
Dunbar, Carmen A.; Callaway, Heather M.; Parrish, Colin R.; Jarrold, Martin F.
Source:
Journal of the American Chemical Society 2018 v.140 no.46 pp. 15701-15711
ISSN:
1520-5126
Subject:
binding capacity,   etc   ; Carnivore protoparvovirus 1; antibodies; antigens; binding sites; capsid; ligands; mass spectrometry; models; monitoring; post-translational modification; proteins; stoichiometry; viruses;     Show all 14 Subjects
Abstract:
... There are many techniques for monitoring and measuring the interactions between proteins and ligands. Most of these techniques are ensemble methods that can provide association constants and in some cases stoichiometry. Here we use charge detection mass spectrometry (CDMS), a single particle technique, to probe the interactions of antigen binding fragments (Fabs) from a series of antibodies with t ...
DOI:
10.1021/jacs.8b08050